Effect of cholestanol and cholesterol-loaded cyclodextrin on stallion sperm function and capacitation post-cryopreservation

Primer Autor
Felmer, Ricardo
Co-autores
Contreras, Maria Jose
Arias, Maria Elena
Silva, Mauricio
Cabrera, Paulina
Título
Effect of cholestanol and cholesterol-loaded cyclodextrin on stallion sperm function and capacitation post-cryopreservation
Editorial
ELSEVIER SCIENCE INC
Revista
THERIOGENOLOGY
Lenguaje
en
Resumen
Cryopreservation of stallion semen is less efficient than other species such as bovine. This is mainly because of the greater susceptibility of stallion sperm to the freezing damage that generates oxidative stress and plasma membrane injury, resulting in DNA fragmentation and cell death.These data suggest the need to develop new strategies of sperm cryopreservation that can improve the efficiency of this technique in stallions by reducing or preventing membrane damage and cell death. The present study aimed to evaluate the effect of adding membrane stabilizers to the freezing medium and assess the quality and in vitro capacitation of stallion sperm after thawing. Semen samples from three stallions frozen with membrane stabilizers (cholesterol-loaded cyclodextrin and cholestanol-loaded cyclodextrin) were evaluated in two experiments: i) sperm quality and functional analysis after thawing, and ii) sperm quality and functional analysis after 4 h of post-thaw incubation in capacitating conditions. Plasma membrane integrity, mitochondrial membrane potential, membrane lipid disorder, intracellular Ca2 thorn , tyrosine phosphorylation, acrosome reaction, DNA damage, sperm motility, and binding to the zona pellucida were assessed. The results showed that cholesterol-loaded cyclodextrin was the stabilizer that most efficiently reduced the membrane disruption and post-thaw cell damage. In addition, this stabilizer made it possible to obtain in vitro capacitated sperm showing higher plasma membrane integrity, mitochondrial membrane potential, sperm motility, binding to the zona pellucida and better response to in vitro capacitating conditions. (c) 2022 Elsevier Inc. All rights reserved.
Tipo de Recurso
artículo original
Description
The authors gratefully acknowledge funding support from FONDECYT 1160467, ANID, Chile, Doctoral scholarship ANID 21181068, Chile, and our local slaughterhouse Frigorifico Temuco for the provision of ovaries.
Los autores agradecen el apoyo financiero del FONDECYT 1160467, ANID, Chile, la beca de doctorado ANID 21181068, Chile y nuestro matadero local Frigorífico Temuco para la provisión de ovarios.
doi
10.1016/j.theriogenology.2022.06.005
Formato Recurso
PDF
Palabras Claves
Stallion sperm
Cryopreservation
Capacitation
Cyclodextrin
Cholestanol
Cholesterol
IN-VITRO FERTILIZATION
TYROSINE PHOSPHORYLATION
COLD SHOCK
SPERMATOZOA
BOAR
BULL
CRYOSURVIVAL
QUALITY
BINDING
Ubicación del archivo
http://dx.doi.org/10.1016/j.theriogenology.2022.06.005
Categoría OCDE
Biología Reproductiva
Ciencias Veterinarias
Materias
Esperma de semental
Crioconservación
Capacitación
Ciclodextrina
Colestanol
Colesterol
FERTILIZACIÓN IN VITRO
FOSFORILACIÓN DE TIROSINA
CHOQUE DE FRÍO
ESPERMATOZOOS
JABALÍ
TORO
CRIOSUPERVIVENCIA
CALIDAD
UNIÓN
Disciplinas de la OCDE
Ciencias Veterinarias
Biología Reproductiva
Ciencia Animal y lechería
Título de la cita (Recomendado-único)
Effect of cholestanol and cholesterol-loaded cyclodextrin on stallion sperm function and capacitation post-cryopreservation
Página de inicio (Recomendado-único)
1
Página final (Recomendado-único)
10
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
Condición de la licencia (Recomendado-repetible)
0
Derechos de acceso
metadata
Access Rights
metadata
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID 21181068
ANID-FONDECYT 1160467
ANID FONDECYT 1160467
Id de Web of Science
WOS:000831544100001
Colecciones
Colección Publicaciones Científicas
Colección ANID
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