Repositorio UFRO · Omeka S

Overexpression of the Tectona grandis TgNAC01 regulates growth, leaf senescence and confer salt stress tolerance in transgenic tobacco plants

Primer Autor	Carrer, Helaine
Co-autores	Matias, Fernando de Oliveira, Perla Novais Gomez-Espinoza, Olman Galeano, Esteban
Título	Overexpression of the Tectona grandis TgNAC01 regulates growth, leaf senescence and confer salt stress tolerance in transgenic tobacco plants
Editorial	PEERJ INC
Revista	PEERJ
Lenguaje	en
Resumen	NAC transcription factors play critical roles in xylem secondary development and in regulation of stress response in plants. NAC proteins related to secondary cell wall development were recently identified and characterized in Tectona grandis (teak), one of the hardwood trees of highest economic importance in the world. In this work, we characterized the novel TgNAC01 gene, which is involved in signaling pathways that mediate teak response to stress. Abscisic acid (ABA) increases TgNAC01 expression in teak plants. Therefore, this gene may have a role in signaling events that mediate ABA-dependent osmotic stress responsive in this plant species. Stable expression in tobacco plants showed that the TgNAC01 protein is localized in the cell nucleus. Overexpression of TgNAC01 in two out three independent transgenic tobacco lines resulted in increased growth, leaf senescence and salt tolerance compared to wild type (WT) plants. Moreover, the stress tolerance of transgenic plants was affected by levels of TgNAC01 gene expression. Water potential, gas exchange and chlorophyll fluorescence were used to determine salt stress tolerance. The 35S:TgNAC01-6 line under 300 mM NaCl stress responded with a significant increase in photosynthesis rate, stomatal conductance, transpiration and carboxylation efficiency, but lower water potential compared to WT plants. The data indicate that the TgNAC01 transcription factor acts as a transcriptional activator of the ABA-mediated regulation and induces leaf senescence.
Tipo de Recurso	artículo original
doi	10.7717/peerj.13039
Formato Recurso	PDF
Palabras Claves	Tropical tree physiology Plant stress Functional analysis Transcription factor NAC TRANSCRIPTION FACTORS GENE-EXPRESSION ARABIDOPSIS FAMILY DOMAIN RESISTANCE NETWORK ANAC019 PCR
Ubicación del archivo	http://dx.doi.org/10.7717/peerj.13039
Categoría OCDE	Ciencias Multidisciplinarias
Materias	Fisiología de árboles tropicales Estrés vegetal Análisis funcional Factor de transcripción FACTORES DE TRANSCRIPCIÓN NAC EXPRESIÓN GENÉTICA ARABIDOPSIS FAMILIA DOMINIO RESISTENCIA RED ANAC019 PCR
Disciplinas de la OCDE	Biotecnología agrícola Biología Molecular Botánica
Título de la cita (Recomendado-único)	Overexpression of the Tectona grandis TgNAC01 regulates growth, leaf senescence and confer salt stress tolerance in transgenic tobacco plants
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	version publicada
Condición de la licencia (Recomendado-repetible)	0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:000767245800002