Overexpression of the Tectona grandis TgNAC01 regulates growth, leaf senescence and confer salt stress tolerance in transgenic tobacco plants

Primer Autor
Carrer, Helaine
Co-autores
Matias, Fernando
de Oliveira, Perla Novais
Gomez-Espinoza, Olman
Galeano, Esteban
Título
Overexpression of the Tectona grandis TgNAC01 regulates growth, leaf senescence and confer salt stress tolerance in transgenic tobacco plants
Editorial
PEERJ INC
Revista
PEERJ
Lenguaje
en
Resumen
NAC transcription factors play critical roles in xylem secondary development and in regulation of stress response in plants. NAC proteins related to secondary cell wall development were recently identified and characterized in Tectona grandis (teak), one of the hardwood trees of highest economic importance in the world. In this work, we characterized the novel TgNAC01 gene, which is involved in signaling pathways that mediate teak response to stress. Abscisic acid (ABA) increases TgNAC01 expression in teak plants. Therefore, this gene may have a role in signaling events that mediate ABA-dependent osmotic stress responsive in this plant species. Stable expression in tobacco plants showed that the TgNAC01 protein is localized in the cell nucleus. Overexpression of TgNAC01 in two out three independent transgenic tobacco lines resulted in increased growth, leaf senescence and salt tolerance compared to wild type (WT) plants. Moreover, the stress tolerance of transgenic plants was affected by levels of TgNAC01 gene expression. Water potential, gas exchange and chlorophyll fluorescence were used to determine salt stress tolerance. The 35S:TgNAC01-6 line under 300 mM NaCl stress responded with a significant increase in photosynthesis rate, stomatal conductance, transpiration and carboxylation efficiency, but lower water potential compared to WT plants. The data indicate that the TgNAC01 transcription factor acts as a transcriptional activator of the ABA-mediated regulation and induces leaf senescence.
Tipo de Recurso
artículo original
doi
10.7717/peerj.13039
Formato Recurso
PDF
Palabras Claves
Tropical tree physiology
Plant stress
Functional analysis
Transcription factor
NAC TRANSCRIPTION FACTORS
GENE-EXPRESSION
ARABIDOPSIS
FAMILY
DOMAIN
RESISTANCE
NETWORK
ANAC019
PCR
Ubicación del archivo
http://dx.doi.org/10.7717/peerj.13039
Categoría OCDE
Ciencias Multidisciplinarias
Materias
Fisiología de árboles tropicales
Estrés vegetal
Análisis funcional
Factor de transcripción
FACTORES DE TRANSCRIPCIÓN NAC
EXPRESIÓN GENÉTICA
ARABIDOPSIS
FAMILIA
DOMINIO
RESISTENCIA
RED
ANAC019
PCR
Disciplinas de la OCDE
Biotecnología agrícola
Biología Molecular
Botánica
Título de la cita (Recomendado-único)
Overexpression of the Tectona grandis TgNAC01 regulates growth, leaf senescence and confer salt stress tolerance in transgenic tobacco plants
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
Condición de la licencia (Recomendado-repetible)
0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:000767245800002
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Colección Publicaciones Científicas
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