Optimization of enzymatic parameters for the production of formononetin from red clover (Trifolium pratense L.) through a response surface methodology

Primer Autor
Quiroz, Andres
Co-autores
Teresa Munoz, Maria
Maldonado, Vanessa
Herrera, Wence
Mutis, Ana
Bardehle, Leonardo
Medina, Cristian
Hormazabal, Emilio
Ortega, Fernando,
Título
Optimization of enzymatic parameters for the production of formononetin from red clover (Trifolium pratense L.) through a response surface methodology
Editorial
TAYLOR & FRANCIS LTD
Revista
NATURAL PRODUCT RESEARCH
Lenguaje
en
Resumen
Trifolium pratense, is a forage found worldwide, but it is negatively impacted by the clover root borer, Hylastinus obscurus. Methanolic extraction has been reported for isolating formononetin from vegetal tissues, with an antifeeding effect on H. obscurus. However, this methodology is time-consuming and also extracts other secondary metabolites, whereas enzymatic assays can provide higher specificity. Hence, the objective of this work was to determine the optimal conditions in pH, temperature, and incubation time for the activity of isoflavone synthase via a response surface model. Once these parameters were optimized, the concentration of formononetin in cultivars and experimental lines of T. pratense was evaluated enzymatically. The results showed that the best condition for developing the enzymatic assay was pH 9.1 with an incubation at 34.5 degrees C for 155 min. The formononetin content fluctuated between 0.74 and 1.96 mg/g of fresh weight, where Precoz-3, Precoz-1, and Superqueli-INIA presented the highest production.
Tipo de Recurso
artículo original
Description
This work was funded by FONDECYT grant 1181697.
Este trabajo fue financiado con el subsidio FONDECYT 1181697.
doi
10.1080/14786419.2021.2002324
Formato Recurso
PDF
Palabras Claves
Red clover (Trifolium pratense L
)
formononetin
enzymatic assay
isoflavone synthase
ISOFLAVONE SYNTHASE
COLEOPTERA
EXPRESSION
Ubicación del archivo
http://dx.doi.org/10.1080/14786419.2021.2002324
Categoría OCDE
Química Aplicada
Química
Medicinal
Materias
Trébol rojo (Trifolium pratense L
)
formononetina
ensayo enzimático
isoflavona sintasa
ISOFLAVONA SINTASEA
COLEOPTERA
EXPRESIÓN
Disciplinas de la OCDE
Agricultura
Fitomejoramiento y protección vegetal
Bioquímica
Título de la cita (Recomendado-único)
Optimization of enzymatic parameters for the production of formononetin from red clover (Trifolium pratense L.) through a response surface methodology
Página de inicio (Recomendado-único)
4719
Página final (Recomendado-único)
4724
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
Condición de la licencia (Recomendado-repetible)
0
Derechos de acceso
metadata
Access Rights
metadata
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID-FONDECYT 1181697
ANID FONDECYT 1181697
Id de Web of Science
WOS:000718684200001
Colecciones
Colección Publicaciones Científicas
Colección ANID
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