Optimization of enzymatic parameters for the production of formononetin from red clover (Trifolium pratense L.) through a response surface methodology
| Primer Autor |
Quiroz, Andres
|
| Co-autores |
Teresa Munoz, Maria
Maldonado, Vanessa
Herrera, Wence
Mutis, Ana
Bardehle, Leonardo
Medina, Cristian
Hormazabal, Emilio
Ortega, Fernando,
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| Título |
Optimization of enzymatic parameters for the production of formononetin from red clover (Trifolium pratense L.) through a response surface methodology
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| Editorial |
TAYLOR & FRANCIS LTD
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| Revista |
NATURAL PRODUCT RESEARCH
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| Lenguaje |
en
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| Resumen |
Trifolium pratense, is a forage found worldwide, but it is negatively impacted by the clover root borer, Hylastinus obscurus. Methanolic extraction has been reported for isolating formononetin from vegetal tissues, with an antifeeding effect on H. obscurus. However, this methodology is time-consuming and also extracts other secondary metabolites, whereas enzymatic assays can provide higher specificity. Hence, the objective of this work was to determine the optimal conditions in pH, temperature, and incubation time for the activity of isoflavone synthase via a response surface model. Once these parameters were optimized, the concentration of formononetin in cultivars and experimental lines of T. pratense was evaluated enzymatically. The results showed that the best condition for developing the enzymatic assay was pH 9.1 with an incubation at 34.5 degrees C for 155 min. The formononetin content fluctuated between 0.74 and 1.96 mg/g of fresh weight, where Precoz-3, Precoz-1, and Superqueli-INIA presented the highest production.
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| Tipo de Recurso |
artículo original
|
| Description |
This work was funded by FONDECYT grant 1181697.
Este trabajo fue financiado con el subsidio FONDECYT 1181697.
|
| doi |
10.1080/14786419.2021.2002324
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| Formato Recurso |
PDF
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| Palabras Claves |
Red clover (Trifolium pratense L
)
formononetin
enzymatic assay
isoflavone synthase
ISOFLAVONE SYNTHASE
COLEOPTERA
EXPRESSION
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| Ubicación del archivo | |
| Categoría OCDE |
Química Aplicada
Química
Medicinal
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| Materias |
Trébol rojo (Trifolium pratense L
)
formononetina
ensayo enzimático
isoflavona sintasa
ISOFLAVONA SINTASEA
COLEOPTERA
EXPRESIÓN
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| Título de la cita (Recomendado-único) |
Optimization of enzymatic parameters for the production of formononetin from red clover (Trifolium pratense L.) through a response surface methodology
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| Página de inicio (Recomendado-único) |
4719
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| Página final (Recomendado-único) |
4724
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| Identificador del recurso (Mandatado-único) |
artículo original
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| Versión del recurso (Recomendado-único) |
version publicada
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| Condición de la licencia (Recomendado-repetible) |
0
|
| Derechos de acceso |
metadata
|
| Access Rights |
metadata
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID-FONDECYT 1181697
ANID FONDECYT 1181697
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| Id de Web of Science |
WOS:000718684200001
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