Acetylcholinesterase inhibitory activity from Amaryllis belladonna growing in Chile: enzymatic and molecular docking studies

Primer Autor
Hormazabal, Emilio
Co-autores
Mella, Monica
Moraga-Nicolas, Felipe
Machuca, Juan
Quiroz, Andres
Mutis, Ana
Becerra, Jose
Astudillo, Alvaro
Título
Acetylcholinesterase inhibitory activity from Amaryllis belladonna growing in Chile: enzymatic and molecular docking studies
Editorial
TAYLOR & FRANCIS LTD
Revista
NATURAL PRODUCT RESEARCH
Lenguaje
en
Resumen
Alkaloid profiles from Amaryllis belladonna plants collected in Chile were examined by GC-MS to assess their inhibitory activity on acetylcholinesterase (AChE) using in vitro and in silico methodologies. The alkaloid extract was roughly separated by column chromatography on silica gel. AChE inhibitory activities from extracts and purified alkaloids were tested by the Ellman method and a molecular docking study was performed to assess the interaction between AChE and purified alkaloids. Sixteen alkaloids were found from hexane and chloroform extracts, and three were isolated and identified as buphanidrine, acetylcaranine and lycorine. Chloroform extract showed the greatest AChE inhibitory activity with IC50 value 8.89 mu g/mL, whereas buphanidrine exhibited the highest inhibitory activity, with IC50 value 17.56 mu g/mL. Inhibition kinetics showed that buphanidrine acts as a mixed inhibitor and molecular docking supports this inhibition mechanism. Overall, our study supports the potential use of A. belladonna as an alkaloid source with AChE inhibitory activity.
Tipo de Recurso
artículo original
doi
10.1080/14786419.2021.1872075
Formato Recurso
PDF
Palabras Claves
Mixed inhibition
buphanidrine
medicinal plant
peripheral anionic site (PAS)
Ubicación del archivo
http://dx.doi.org/10.1080/14786419.2021.1872075
Categoría OCDE
Química Aplicada
Química
Medicinal
Materias
Inhibición mixta
bufanidrina
planta medicinal
sitio aniónico periférico (PAS)
Disciplinas de la OCDE
Botánica
Química Analítica
Farmacología y Farmacia
Versión del recurso (Recomendado-único)
version publicada
Tipo de ruta
hibrida
verde
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WOS:000608339900001
Título de la cita (Recomendado-único)
Acetylcholinesterase inhibitory activity from Amaryllis belladonna growing in Chile: enzymatic and molecular docking studies
Página de inicio (Recomendado-único)
1370
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1374
Identificador del recurso (Mandatado-único)
artículo original
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0
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