Repositorio
Institucional

Pavez, Monica

Troncoso, Claudia

Cerda, Alvaro

Manriquez, Victor

Prado, Aurora

Hofmann, Edmundo

Rios, Eddy

Sierralta, Armando

Copelli, Luis

Barrientos, Leticia

Association of Progranulin Gene Expression from Dyspeptic Patients with Virulent Helicobacter pylori Strains, In Vivo Model

MDPI

MICROORGANISMS

en

(1) Background: Gastric cancer, the fourth most common cause of death from tumors in the world, is closely associated with Helicobacter pylori. Timely diagnosis, therefore, is essential to achieve a higher survival rate. In Chile, deaths from gastric cancer are high, mainly due to late diagnosis. Progranulin has reflected the evolution of some cancers, but has been poorly studied in gastric lesions. Aiming to understand the role of progranulin in H. pylori infection and its evolution in development of gastric lesions, we evaluated the genic expression of progranulin in gastric tissue from infected and non-infected patients, comparing it according to the epithelial status and virulence of H. pylori strains. (2) Methods: The genic expression of progranulin by q-PCR was quantified in gastric biopsies from Chilean dyspeptic patients (n = 75) and individuals who were uninfected (n = 75) by H. pylori, after receiving prior informed consent. Bacteria were grown on a medium Columbia agar with equine-blood 7%, antibiotics (Dent 2%, Oxoid (TM)), in a microaerophilic environment, and genetically characterized for the ureC, vacA, cagA, and iceA genes by PCR. The status of the tissue was determined by endoscopic observation. (3) Results: Minor progranulin expression was detected in atrophic tissue, with a sharp drop in the tissue colonized by H. pylori that carried greater virulence, VacAs1m1(+)CagA(+)IceA1(+). (4) Conclusions: Progranulin shows a differential behavior according to the lesions and virulence of H. pylori, affecting the response of progranulin against gastric inflammation.

artículo original

"This work was funded by the Fund for the Promotion of Scientific and Technological Development (FONDEF), grant XIII CONCURSO FONIS 2016 SA610197 and ""National Fund for Scientific and Technological Development FONDECYT Nffi 11191199 to M.P; NATIONAL COMMISSION OF SCIENCE AND TECHNOLOGY (CONICYT), grant CONICYT-PFCHA/2017-21171513 to C.T.; and the RESEARCH NETWORK PROJECT IN EXTREME ENVIRONMENTS (NEXER), grant NXR17-0003 to L.B.

Este trabajo fue financiado por el Fondo de Fomento al Desarrollo Científico y Tecnológico (FONDEF), beca XIII CONCURSO FONIS 2016 SA610197 y “Fondo Nacional de Desarrollo Científico y Tecnológico FONDECYT Nffi 11191199 al M.P; COMISIÓN NACIONAL DE CIENCIA Y TECNOLOGÍA (CONICYT), concesión CONICYT-PFCHA/2017-21171513 a C.T.; y el PROYECTO RED DE INVESTIGACIÓN EN AMBIENTES EXTREMOS (NEXER), concesión NXR17-0003 a L.B."

10.3390/microorganisms10050998

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progranulin

Helicobacter pylori

virulence

gastric lesions

NF-KAPPA-B

CAGA

PATHOGENESIS

MACROPHAGES

PERSISTENCE

GASTRITIS

STOMACH

HEALTH

Microbiología

progranulina

Helicobacter pylori

virulencia

lesiones gástricas

NF-KAPPA-B

CAGA

PATOGENIA

MACRÓFAGOS

PERSISTENCIA

GASTRITIS

ESTOMAGO

SALUD

Gastroenterología y Hepatología

Biología Molecular

Oncología

version publicada

dorada

verde

acceso abierto

acceso abierto

WOS:000801956400001

Association of Progranulin Gene Expression from Dyspeptic Patients with Virulent Helicobacter pylori Strains, In Vivo Model

artículo original

CC BY 4.0

CC BY 4.0

ANID FONDEF SA610197

ANID FONDECYT 11191199