Association of Progranulin Gene Expression from Dyspeptic Patients with Virulent Helicobacter pylori Strains, In Vivo Model

Primer Autor
Pavez, Monica
Co-autores
Troncoso, Claudia
Cerda, Alvaro
Manriquez, Victor
Prado, Aurora
Hofmann, Edmundo
Rios, Eddy
Sierralta, Armando
Copelli, Luis
Barrientos, Leticia
Título
Association of Progranulin Gene Expression from Dyspeptic Patients with Virulent Helicobacter pylori Strains, In Vivo Model
Editorial
MDPI
Revista
MICROORGANISMS
Lenguaje
en
Resumen
(1) Background: Gastric cancer, the fourth most common cause of death from tumors in the world, is closely associated with Helicobacter pylori. Timely diagnosis, therefore, is essential to achieve a higher survival rate. In Chile, deaths from gastric cancer are high, mainly due to late diagnosis. Progranulin has reflected the evolution of some cancers, but has been poorly studied in gastric lesions. Aiming to understand the role of progranulin in H. pylori infection and its evolution in development of gastric lesions, we evaluated the genic expression of progranulin in gastric tissue from infected and non-infected patients, comparing it according to the epithelial status and virulence of H. pylori strains. (2) Methods: The genic expression of progranulin by q-PCR was quantified in gastric biopsies from Chilean dyspeptic patients (n = 75) and individuals who were uninfected (n = 75) by H. pylori, after receiving prior informed consent. Bacteria were grown on a medium Columbia agar with equine-blood 7%, antibiotics (Dent 2%, Oxoid (TM)), in a microaerophilic environment, and genetically characterized for the ureC, vacA, cagA, and iceA genes by PCR. The status of the tissue was determined by endoscopic observation. (3) Results: Minor progranulin expression was detected in atrophic tissue, with a sharp drop in the tissue colonized by H. pylori that carried greater virulence, VacAs1m1(+)CagA(+)IceA1(+). (4) Conclusions: Progranulin shows a differential behavior according to the lesions and virulence of H. pylori, affecting the response of progranulin against gastric inflammation.
Tipo de Recurso
artículo original
doi
10.3390/microorganisms10050998
Formato Recurso
PDF
Palabras Claves
progranulin
Helicobacter pylori
virulence
gastric lesions
NF-KAPPA-B
CAGA
PATHOGENESIS
MACROPHAGES
PERSISTENCE
GASTRITIS
STOMACH
HEALTH
Ubicación del archivo
http://dx.doi.org/10.3390/microorganisms10050998
Categoría OCDE
Microbiología
Materias
progranulina
Helicobacter pylori
virulencia
lesiones gástricas
NF-KAPPA-B
CAGA
PATOGENIA
MACRÓFAGOS
PERSISTENCIA
GASTRITIS
ESTOMAGO
SALUD
Título de la cita (Recomendado-único)
Association of Progranulin Gene Expression from Dyspeptic Patients with Virulent Helicobacter pylori Strains, In Vivo Model
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:000801956400001
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID FONDEF SA610197
ANID FONDECYT 11191199
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