Production of Carotenoids and Phospholipids by Thraustochytrium sp. in Batch and Repeated-Batch Culture
| Primer Autor |
Shene, Carolina
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| Co-autores |
Leyton, Allison
Chisti, Yusuf
Asenjo, Juan A.
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| Título |
Production of Carotenoids and Phospholipids by Thraustochytrium sp. in Batch and Repeated-Batch Culture
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| Editorial |
MDPI
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| Revista |
MARINE DRUGS
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| Lenguaje |
en
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| Resumen |
The carotenogenic thraustochytrid Thraustochytrium sp. RT2316-16 was grown in batch and repeated-batch cultures using different feeds containing glucose, or glycerol, and yeast extract, for the production of lipids, phospholipids and carotenoids. RT2316-16 produced canthaxanthin, astaxanthin and beta-carotene. The effects of biotin, ascorbic acid, light and temperature were evaluated in some of the experiments. In 2-day-old batch cultures, the combined mass percentage of eicosapentaenoic acid and docosahexaenoic acid in total lipids was between 16.5% (glycerol-based medium in the dark, biomass concentration = 4.2 +/- 1.1 g L-1) and 42.6% (glucose-based medium under light, biomass concentration = 3.3 +/- 0.1 g L-1), decreasing to 3.8% and 6.1%, respectively, after day 4. In repeated-batch cultures, the total lipids in the biomass increased after glucose or glycerol was fed alone, whereas the total carotenoids (168 +/- 7 mu g g(-1) dry weight (DW)) and phospholipids in the biomass increased after feeding with yeast extract. The biomass with the highest content of phospholipids (28.7 +/- 4.3 mg g(-1) DW) was obtained using a feed medium formulated with glycerol, yeast extract and ascorbic acid. Glycerol was the best carbon source for the production of a biomass enriched with total lipids (467 +/- 45 mg g(-1) DW). The composition of carotenoids depended strongly on the composition of the feed. Repeated-batch cultures fed with yeast extract contained canthaxanthin as the main carotenoid, whereas in the cultures fed only with glucose, the biomass contained mainly beta-carotene.
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| Tipo de Recurso |
artículo original
|
| Description |
This research was funded by Conicyt, Fondecyt 1200642 and Centre for Biotechnology and Bioengineering (CeBiB) FB-0001.
Esta investigación fue financiada por Conicyt, Fondecyt 1200642 y el Centro de Biotecnología y Bioingeniería (CeBiB) FB-0001.
|
| doi |
10.3390/md20070416
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| Formato Recurso |
PDF
|
| Palabras Claves |
Thraustochytrium sp
thraustochytrids
carotenoids
canthaxanthin
docosahexaenoic acid
phospholipids
DOCOSAHEXAENOIC ACID
LIPID DROPLETS
GROWTH
SURFACE
STRAIN
DHA
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| Ubicación del archivo | |
| Categoría OCDE |
Química Medicinal
Farmacología y farmacia
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| Materias |
Thraustochytrium sp
thraustochytrids
carotenoides
cantaxantina
ácido docosahexaenoico
fosfolípidos
ÁCIDO DOCOSAHEXAENOICO
GOTAS DE LÍPIDOS
CRECIMIENTO
SUPERFICIE
CEPA
DHA
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| Disciplinas de la OCDE |
Biotecnología Industrial
Bioproductos
Microbiología
|
| Título de la cita (Recomendado-único) |
Production of Carotenoids and Phospholipids by Thraustochytrium sp. in Batch and Repeated-Batch Culture
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
version publicada
|
| License |
CC BY 4.0
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| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
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| Access Rights |
acceso abierto
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
CEBIB FB-0001
ANID-FONDECYT 1200642
ANID FONDECYT 1200642
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| Id de Web of Science |
WOS:000833148300001
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