Repositorio UFRO · Omeka S

Production of Carotenoids and Phospholipids by Thraustochytrium sp. in Batch and Repeated-Batch Culture

Primer Autor	Shene, Carolina
Co-autores	Leyton, Allison Chisti, Yusuf Asenjo, Juan A.
Título	Production of Carotenoids and Phospholipids by Thraustochytrium sp. in Batch and Repeated-Batch Culture
Editorial	MDPI
Revista	MARINE DRUGS
Lenguaje	en
Resumen	The carotenogenic thraustochytrid Thraustochytrium sp. RT2316-16 was grown in batch and repeated-batch cultures using different feeds containing glucose, or glycerol, and yeast extract, for the production of lipids, phospholipids and carotenoids. RT2316-16 produced canthaxanthin, astaxanthin and beta-carotene. The effects of biotin, ascorbic acid, light and temperature were evaluated in some of the experiments. In 2-day-old batch cultures, the combined mass percentage of eicosapentaenoic acid and docosahexaenoic acid in total lipids was between 16.5% (glycerol-based medium in the dark, biomass concentration = 4.2 +/- 1.1 g L-1) and 42.6% (glucose-based medium under light, biomass concentration = 3.3 +/- 0.1 g L-1), decreasing to 3.8% and 6.1%, respectively, after day 4. In repeated-batch cultures, the total lipids in the biomass increased after glucose or glycerol was fed alone, whereas the total carotenoids (168 +/- 7 mu g g(-1) dry weight (DW)) and phospholipids in the biomass increased after feeding with yeast extract. The biomass with the highest content of phospholipids (28.7 +/- 4.3 mg g(-1) DW) was obtained using a feed medium formulated with glycerol, yeast extract and ascorbic acid. Glycerol was the best carbon source for the production of a biomass enriched with total lipids (467 +/- 45 mg g(-1) DW). The composition of carotenoids depended strongly on the composition of the feed. Repeated-batch cultures fed with yeast extract contained canthaxanthin as the main carotenoid, whereas in the cultures fed only with glucose, the biomass contained mainly beta-carotene.
Tipo de Recurso	artículo original
Description	This research was funded by Conicyt, Fondecyt 1200642 and Centre for Biotechnology and Bioengineering (CeBiB) FB-0001. Esta investigación fue financiada por Conicyt, Fondecyt 1200642 y el Centro de Biotecnología y Bioingeniería (CeBiB) FB-0001.
doi	10.3390/md20070416
Formato Recurso	PDF
Palabras Claves	Thraustochytrium sp thraustochytrids carotenoids canthaxanthin docosahexaenoic acid phospholipids DOCOSAHEXAENOIC ACID LIPID DROPLETS GROWTH SURFACE STRAIN DHA
Ubicación del archivo	http://dx.doi.org/10.3390/md20070416
Categoría OCDE	Química Medicinal Farmacología y farmacia
Materias	Thraustochytrium sp thraustochytrids carotenoides cantaxantina ácido docosahexaenoico fosfolípidos ÁCIDO DOCOSAHEXAENOICO GOTAS DE LÍPIDOS CRECIMIENTO SUPERFICIE CEPA DHA
Disciplinas de la OCDE	Biotecnología Industrial Bioproductos Microbiología
Título de la cita (Recomendado-único)	Production of Carotenoids and Phospholipids by Thraustochytrium sp. in Batch and Repeated-Batch Culture
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	version publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Referencia del Financiador (Mandatado si es aplicable-repetible)	CEBIB FB-0001 ANID-FONDECYT 1200642 ANID FONDECYT 1200642
Id de Web of Science	WOS:000833148300001