Cytotoxic Cyclotides from Anchietea pyrifolia, a South American Plant Species

Primer Autor
Bolzani, Vanderlan da Silva
Co-autores
Fernandez-Bobey, Antonio
Ferreira Pinto, Meri Emili
de Almeida, Larissa Costa
de Souza, Bibiana Monson
Dias, Nathalia Baptista
De Paula-Souza, Juliana
Cilli, Eduardo Maffud
Lopes, Norberto Peporine
Costa-Lotufo, Leticia Veras
Palma, Mario Sergio
Título
Cytotoxic Cyclotides from Anchietea pyrifolia, a South American Plant Species
Editorial
AMER CHEMICAL SOC
Revista
JOURNAL OF NATURAL PRODUCTS
Lenguaje
en
Resumen
Cyclotides are mini-proteins with potent bioactivities and outstanding potential for agricultural and pharmaceutical applications. More than 450 different plant cyclotides have been isolated from six angiosperm families. In Brazil, studies involving this class of natural products are still scarce, despite its rich floristic diversity. Herein were investigated the cyclotides from Anchietea pyrifolia roots, a South American medicinal plant from the family Violaceae. Fourteen putative cyclotides were annotated by LC-MS. Among these, three new bracelet cyclotides, anpy A-C, and the known cycloviolacins O4 (cyO4) and O17 (cyO17) were sequenced through a combination of chemical and enzymatic reactions followed by MALDI-MS/MS analysis. Their cytotoxic activity was evaluated by a cytotoxicity assay against three human cancer cell lines (colorectal carcinoma cells: HCT 116 and HCT 116 TP53(-/-) and breast adenocarcinoma, MCF 7). For all assays, the IC50 values of isolated compounds ranged between 0.8 and 7.3 mu M. CyO17 was the most potent cyclotide for the colorectal cancer cell lines (IC50, 0.8 and 1.2 mu M). Furthermore, the hemolytic activity of anpy A and B, cyO4, and cyO17 was assessed, and the cycloviolacins were the least hemolytic (HD50 > 156 mu M). This work sheds light on the cytotoxic effects of the anpy cyclotides against cancer cells. Moreover, this study expands the number of cyclotides obtained to date from Brazilian plant biodiversity and adds one more genus containing these molecules to the list of the Violaceae family.
Tipo de Recurso
artículo original
doi
10.1021/acs.jnatprod.1c01129
Formato Recurso
PDF
Palabras Claves
CYCLOVIOLACIN O2
KNOTTED PROTEINS
CYCLIC-PEPTIDES
VIOLA-ODORATA
DISCOVERY
SEQUENCE
BIFLORA
DEFENSE
FAMILY
TOXINS
Ubicación del archivo
http://dx.doi.org/10.1021/acs.jnatprod.1c01129
Categoría OCDE
Ciencias Vegetales
Química Medicinal
Farmacología y farmacia
Materias
CICLOVIOLACINA O2
PROTEÍNAS NUDAS
PEPTIDOS-CICLICOS
VIOLA-ODORATA
DESCUBRIMIENTO
SECUENCIA
BIFLORA
DEFENSA
FAMILIA
TOXINAS
Disciplinas de la OCDE
Bioquímica
Biotecnología Relacionada con la Salud
Botánica
Título de la cita (Recomendado-único)
Cytotoxic Cyclotides from Anchietea pyrifolia, a South American Plant Species
Página de inicio (Recomendado-único)
2127
Página final (Recomendado-único)
2134
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
Condición de la licencia (Recomendado-repetible)
0
Derechos de acceso
metadata
Access Rights
metadata
Id de Web of Science
WOS:000852049500001
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