Repositorio
Institucional

Herrera, Hector

Sanhueza, Tedy

da Silva Valadares, Rafael Borges

Matus, Francisco

Pereira, Guillermo

Atala, Cristian

de la Luz Mora, Maria

Arriagada, Cesar

Diversity of Root-Associated Fungi of the Terrestrial Orchids Gavilea lutea and Chloraea collicensis in a Temperate Forest Soil of South-Central Chile

MDPI

JOURNAL OF FUNGI

en

The diversity of orchid mycorrhizal fungi (OMF) and other beneficial root-associated fungi in temperate forests has scarcely been examined. This study aimed to analyze the diversity of mycorrhizal and rhizosphere-associated fungal communities in the terrestrial orchids Gavilea lutea and Chloraea collicensis growing in high-orchid-population-density areas in the piedmont of the Andes Cordillera with native forest (Nothofagus-Araucaria) and Coastal Cordillera with an exotic plantation (Pinus-Eucalyptus) in south-central Chile. We focused on rhizosphere-inhabiting and peloton-associated OMF in a native forest (Andes Cordillera) and a mixed forest (Coastal Cordillera). The native terrestrial orchids G. lutea and C. collicensis were localized, mycorrhizal root segments were taken to isolate peloton-associated OMF, and rhizosphere soil was taken to perform the metabarcoding approach. The results revealed that Basidiomycota and Ascomycota were the main rhizosphere-inhabiting fungal phyla, showing significant differences in the composition of fungal communities in both sites. Sebacina was the most-abundant OMF genera in the rhizosphere of G. lutea growing in the native forest soil. In contrast, Thanatephorus was the most abundant mycorrhizal taxa growing in the rhizosphere of orchids from the Coastal Cordillera. Besides, other OMF genera such as Inocybe, Tomentella, and Mycena were detected. The diversity of OMF in pelotons differed, being mainly related to Ceratobasidium sp. and Tulasnella sp. These results provide evidence of differences in OMF from pelotons and the rhizosphere soil in G. lutea growing in the Andes Cordillera and a selection of microbial communities in the rhizosphere of C. collicensis in the Coastal Cordillera. This raises questions about the efficiency of propagation strategies based only on mycorrhizal fungi obtained by culture-dependent methods, especially in orchids that depend on non-culturable taxa for seed germination and plantlet development.

artículo original

This research was funded by FONDECYT-POSTDOCTORADO 3200134 and FONDECYT-REGULAR 1211857.

Esta investigación fue financiada por FONDECYT-POSTDOCTORADO 3200134 y FONDECYT-REGULAR 1211857.

10.3390/jof8080794

PDF

mycoheterotrophy

Orchidaceae

orchid mycorrhizae

soil fungi

symbiosis

MYCORRHIZAL FUNGI

CARBON

ANDES

GERMINATION

GENERATION

NITROGEN

RANGE

Microbiología

Micología

micoheterotrofia

Orchidaceae

micorrizas de orquídeas

hongos del suelo

simbiosis

HONGOS MICORRIZALES

CARBONO

ANDES

GERMINACIÓN

GENERACIÓN

NITRÓGENO

RANGO

Diversity of Root-Associated Fungi of the Terrestrial Orchids Gavilea lutea and Chloraea collicensis in a Temperate Forest Soil of South-Central Chile

artículo original

version publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

ANID-FONDECYT 3200134

ANID-FONDECYT 1211857

ANID FONDECYT 3200134

ANID FONDECYT 1211857

WOS:000845789500001