Repositorio
Institucional

Benimeli, Claudia S.

Saez, Juliana M.

Gonzalez, Samanta K.

Ocante, Teresa A. L.

Bigliardo, Ana L.

Briceno, Gabriela E.

Actinobacteria bioaugmentation and substrate evaluation for biobeds useful for the treatment of atrazine residues in agricultural fields

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD

JOURNAL OF ENVIRONMENTAL MANAGEMENT

en

Biopurification systems (BPS) or biobeds are bioprophylaxis systems to prevent pesticide point-source contam-ination, whose efficiency relies mostly on the pesticide removal capacity of the biomixture, the majority component of a BPS. The adaptation of the components of the biomixtures to local availabilities is a key aspect to ensure the sustainability of the system. In this work, the removal of atrazine (ATZ) was evaluated in biomixtures formulated with three sugarcane by-products as alternative lignocellulosic substrates. Based on the capacity of actinobacteria to tolerate and degrade diverse pesticides, the effect of biomixtures bioaugmentation with acti-nobacteria was evaluated as a strategy to enhance the depuration capacity of biobeds. Also, the effect of ATZ and/or the bioaugmentation on microbial developments and enzymatic activities were studied. The biomixtures formulated with bagasse, filter cake, or harvest residue, reached pesticide removal values of 37-41% at 28 d of incubation, with t1/2 between 37.9 +/- 0.4 d and 52.3 +/- 0.4 d. The bioaugmentation with Streptomyces sp. M7 accelerated the dissipation of the pesticide in the biomixtures, reducing ATZ t1/2 3-fold regarding the controls, and achieving up to 72% of ATZ removal. Atrazine did not exert a clear effect on microbial developments, although most of the microbial counts were less in the contaminated biomixtures at the end of the assay. The bioaugmentation improved the development of the microbiota in general, specially actinobacteria and fungi, regarding the non-bioaugmented systems. The inoculation with Streptomyces sp. M7 enhanced acid phosphatase activity and/or reversed a possible effect of the pesticide over this enzymatic activity.

artículo original

This work was supported by Agencia Nacional de Promocion Cientifica y Tecnologica (PICT 2016 -0493, PICT 2016 -0234), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET, PIP 2017 -683), Secretaria de Ciencia, Arte e Innovacion Tecnologica (SCAIT, PIUNT D504, Proyectos Orientados a Temas Estrategicos SCAIT- UNT 2020), and ANID-REDES Project 190020. The authors gratefully acknowledge Mr. Raul Perez (Ingenio Cruz Alta, Los Balcanes S.A.) for providing sugarcane by-products.

10.1016/j.jenvman.2022.115870

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Biomixture

Pesticides

Atrazine

Actinobacteria

Bioaugmentation

Biopurification system

STREPTOMYCES SP M7

CHLORPYRIFOS DEGRADATION

BIOPURIFICATION SYSTEMS

FUNGAL BIOAUGMENTATION

PESTICIDE REMOVAL

LINDANE REMOVAL

SOIL

DISSIPATION

BIOREMEDIATION

BIOMIXTURE

Ciencias Ambientales

Biomezcla

Pesticidas

Atrazina

Actinobacteria

Bioaumento

Sistema de biopurificación

STREPTOMYCES SP M7

DEGRADACIÓN DE CLORPIRIFOS

SISTEMAS DE BIOPURIFICACIÓN

BIOAUMENTACIÓN DE HUNGOS

ELIMINACIÓN DE PESTICIDAS

ELIMINACIÓN DE LINDANO

SUELO

DISIPACIÓN

BIORREMEDIACIÓN

BIOMEZCLA

Actinobacteria bioaugmentation and substrate evaluation for biobeds useful for the treatment of atrazine residues in agricultural fields

artículo original

version publicada

0

metadata

metadata

ANPCyT PICT 2016 -0493

ANPCyT PICT 2016 -0234

CONICET PIP 2017 -683

ANID-REDES 190020

SCAIT PIUNT D504

ANID REDES 190020

WOS:000879730100003