Aislación y Caracterización de las Células Epiteliales del Amnios

Primer Autor
Bucchi, Cristina
Co-autores
De Anta, Josep Maria
Título
Aislación y Caracterización de las Células Epiteliales del Amnios
Editorial
SOC CHILENA ANATOMIA
Revista
INTERNATIONAL JOURNAL OF MORPHOLOGY
Lenguaje
es
Resumen
human amnion epithelial cells (hAECs) are pluripotent stem cells, they have the ability to differentiate into cells of the three embryonic layers, and are used in various regenerative therapies in medicine. This study aims to describe a protocol for the isolation of amnion epithelial cells (hAECs) from human placentas from cesarean delivery, as well as their characterization and culture conditions in vitro. hAECs were isolated from 20 cesarean delivery placentas with an optimized protocol. The cells were characterized by flow cytometry, light and fluorescence microscopy, and the proliferation of the cells was evaluated by MTT at 1, 3, 5 and 7 days with and without b-mercaptoethanol in the culture medium. Histological analysis of the amnion showed a practically complete detachment of the cells of the underlying membrane after the second digestion. The average number of cells obtained was 10.97 million cells per amnion. The hAECs perform a limited proliferation rate, which was not favored by the addition of beta-mercaptoethanol in the culture. A spontaneous morphology change from epithelial to mesenchymal morphology is exhibited after the fourth passage. The epithelial cells of the amnion can be isolated with a simple and effective protocol, however, they present little proliferative capacity. Under the conditions of this study, the addition of beta-mercaptoethanol does not favor the proliferation of the cells.
Tipo de Recurso
artículo original
doi
10.4067/S0717-95022022000300817
Formato Recurso
PDF
Palabras Claves
human amnion epithelial cells
pluripotent cells
isolation protocol
MESENCHYMAL STROMAL CELLS
MEMBRANE TRANSPLANTATION
Ubicación del archivo
http://dx.doi.org/10.4067/S0717-95022022000300817
Categoría OCDE
Anatomía y Morfología
Materias
Células epiteliales del amnios humano
células pluripotentes
protocolo de aislamiento
CÉLULAS DEL ESTROMA MESENQUIMAL
TRASPLANTE DE MEMBRANA
Título de la cita (Recomendado-único)
Aislación y Caracterización de las Células Epiteliales del Amnios
Página de inicio (Recomendado-único)
817
Página final (Recomendado-único)
823
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
License
CC BY-NC 4.0
Condición de la licencia (Recomendado-repetible)
CC BY-NC 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:000830996700042
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Colección Publicaciones Científicas
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