Repositorio
Institucional

Bucchi, Cristina

De Anta, Josep Maria

Aislación y Caracterización de las Células Epiteliales del Amnios

SOC CHILENA ANATOMIA

INTERNATIONAL JOURNAL OF MORPHOLOGY

es

human amnion epithelial cells (hAECs) are pluripotent stem cells, they have the ability to differentiate into cells of the three embryonic layers, and are used in various regenerative therapies in medicine. This study aims to describe a protocol for the isolation of amnion epithelial cells (hAECs) from human placentas from cesarean delivery, as well as their characterization and culture conditions in vitro. hAECs were isolated from 20 cesarean delivery placentas with an optimized protocol. The cells were characterized by flow cytometry, light and fluorescence microscopy, and the proliferation of the cells was evaluated by MTT at 1, 3, 5 and 7 days with and without b-mercaptoethanol in the culture medium. Histological analysis of the amnion showed a practically complete detachment of the cells of the underlying membrane after the second digestion. The average number of cells obtained was 10.97 million cells per amnion. The hAECs perform a limited proliferation rate, which was not favored by the addition of beta-mercaptoethanol in the culture. A spontaneous morphology change from epithelial to mesenchymal morphology is exhibited after the fourth passage. The epithelial cells of the amnion can be isolated with a simple and effective protocol, however, they present little proliferative capacity. Under the conditions of this study, the addition of beta-mercaptoethanol does not favor the proliferation of the cells.

artículo original

10.4067/S0717-95022022000300817

PDF

human amnion epithelial cells

pluripotent cells

isolation protocol

MESENCHYMAL STROMAL CELLS

MEMBRANE TRANSPLANTATION

Anatomía y Morfología

Células epiteliales del amnios humano

células pluripotentes

protocolo de aislamiento

CÉLULAS DEL ESTROMA MESENQUIMAL

TRASPLANTE DE MEMBRANA

Biotecnología Relacionada con la Salud

Biología Celular

Otros Temas de Medicina Básica

version publicada

dorada

verde

acceso abierto

acceso abierto

WOS:000830996700042

Aislación y Caracterización de las Células Epiteliales del Amnios

817

823

artículo original

CC BY-NC 4.0

CC BY-NC 4.0