Evaluación de la Mineralización en Mandíbula y Diente de Trucha Arcoíris (Oncorhynchus mykiss) Mediante Microscopía Electrónica de Barrido de Presión Variable Acoplada a Detector de Espectroscopía de Rayos X de Energía Dispersiva

Primer Autor
Rojas, Mariana
Co-autores
Godoy, Karina
Sandoval, Cristian
Manterola-Barroso, Carlos
Salinas, Paulo
Salazar, Luis A.
Título
Evaluación de la Mineralización en Mandíbula y Diente de Trucha Arcoíris (Oncorhynchus mykiss) Mediante Microscopía Electrónica de Barrido de Presión Variable Acoplada a Detector de Espectroscopía de Rayos X de Energía Dispersiva
Editorial
SOC CHILENA ANATOMIA
Revista
INTERNATIONAL JOURNAL OF MORPHOLOGY
Lenguaje
es
Resumen
In salmon farming systems, X-ray images are used to evaluate the spine and fins, but these do not allow quantifying the minerals that make up the bone, for different techniques that are destructive are used. Variable Pressure Scanning Electron Microscopy (VP SEM) coupled to an Energy Dispersive X-ray spectroscopy detector (EDX), allows us to analyze the microstructure and at the same time determine chemical elements, percentages, and distribution present at specific points in a sample. Five control and five jaw deformity trout, from freshwater and in the smolt stage were used. The structure and mineralization of the bone were analyzed, an average six quantification points were chosen per region of interest (ROI) and then they were analyzed by Scanning Electron Microscopy (VP SEM-EDX). The data obtained have shown the average mass percentage of calcium in trout joint bone was 8.07 % and 14.48 % in jaw deformity and control trout, respectively, and the average mass percentage of phosphorus is 4.07 % and 7.60 %, in jaw deformity and control trout, respectively. Other elements present in the sample were also identified, such as magnesium, sodium, carbon, oxygen, nitrogen, sulphur, fluorine, zinc, aluminium, and iron, with special interest the increase of carbon in the analyzed samples with mandibular deformity and the presence of aluminum in all samples. The VP SEM-EDX Technique allows direct evaluation, without destruction of the sample and with minimal sample preparation. In bone, the most frequent application of SEM-EDX is the measurement of the content of calcium (Ca) and phosphorus (P) and the relationship that exists between these elements, calcium/phosphorus (Ca/P), in the sample. At the same time, the technique allows us to detect other microelements from water or food that can eventually cause alterations in fish, confirming the hypothesis that elemental microanalysis is useful for salmon farming.
Tipo de Recurso
artículo original
doi
10.4067/S0717-95022022000200530
Formato Recurso
PDF
Palabras Claves
X-ray Spectroscopy (EDX)
Bone
Rainbow Trout
CHONDROCYTE DIFFERENTIATION
SIGNALING PATHWAYS
FISH
OSTEOBLAST
INTEGRATION
CALCIUM
BMP
Ubicación del archivo
http://dx.doi.org/10.4067/S0717-95022022000200530
Categoría OCDE
Anatomía y Morfología
Materias
Espectroscopia de rayos X (EDX)
Hueso
Trucha arcoíris
DIFERENCIACIÓN DE CONDROCITOS
VÍAS DE SEÑALIZACIÓN
PESCADO
OSTEOBLAST
INTEGRACIÓN
CALCIO
BMP
Disciplinas de la OCDE
Pesca
Ciencias Veterinarias
Química Analítica
Versión del recurso (Recomendado-único)
version publicada
Tipo de ruta
dorada
verde
Access Rights
acceso abierto
Derechos de acceso
acceso abierto
Id de Web of Science
WOS:000800856600038
Título de la cita (Recomendado-único)
Evaluación de la Mineralización en Mandíbula y Diente de Trucha Arcoíris (Oncorhynchus mykiss) Mediante Microscopía Electrónica de Barrido de Presión Variable Acoplada a Detector de Espectroscopía de Rayos X de Energía Dispersiva
Página de inicio (Recomendado-único)
530
Página final (Recomendado-único)
539
Identificador del recurso (Mandatado-único)
artículo original
License
CC BY-NC 4.0
Condición de la licencia (Recomendado-repetible)
CC BY-NC 4.0
Colecciones
Colección Publicaciones Científicas
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