MicroRNA-20a-5p Downregulation by Atorvastatin: A Potential Mechanism Involved in Lipid-Lowering Therapy
| Primer Autor |
Salazar, Luis A.
|
| Co-autores |
Saavedra, Kathleen
Leal, Karla
Saavedra, Nicolas
Prado, Yalena
Paez, Isis
Ubilla, Carmen G.
Rojas, Gabriel
|
| Título |
MicroRNA-20a-5p Downregulation by Atorvastatin: A Potential Mechanism Involved in Lipid-Lowering Therapy
|
| Editorial |
MDPI
|
| Revista |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
|
| Lenguaje |
en
|
| Resumen |
The treatment of hypercholesterolemia is mainly based on statins. However, the response to pharmacological therapy shows high inter-individual variability, resulting in variable effects in both lipid lowering and risk reduction. Thus, a better understanding of the lipid-lowering mechanisms and response variability at the molecular level is required. Previously, we demonstrated a deregulation of the microRNA expression profile in HepG2 cells treated for 24 h with atorvastatin, using a microarray platform. In the present study, we evaluated the expression of hsa-miR-17-5p, hsa-miR-20a-5p and hsa-miR-106a-5p in hypercholesterolemic patients before and after atorvastatin treatment and in HepG2 cells treated for 24 h with atorvastatin The miRNA hsa-mir-20a-5p was repressed after atorvastatin treatment in hypercholesteremic subjects and in HepG2 cells in culture. Repression of hsa-mir-20a-5p increased LDLR gene and protein expression in HepG2 cells, while hsa-mir-20a-5p overexpression reduced LDLR gene and protein expression.
|
| Tipo de Recurso |
artículo original
|
| Description |
This research was funded by Fondecyt-Chile (Grant numbers 3160567 and 1171765), Direccion de Investigacion of the Universidad de La Frontera (Grant number DI19-0094), and ANIDChile Doctoral Scholarship number 21181008.
|
| doi |
10.3390/ijms23095022
|
| Formato Recurso |
PDF
|
| Palabras Claves |
microRNA
statin
hypercholesterolemia
cardiovascular diseases
atorvastatin
DENSITY-LIPOPROTEIN CHOLESTEROL
CORONARY-ARTERY-DISEASE
HEPATOCELLULAR-CARCINOMA
CARDIOVASCULAR-DISEASE
CHILEAN SUBJECTS
EXPRESSION
STATINS
CELLS
BIOMARKERS
MIR-17-5P
|
| Ubicación del archivo | |
| Categoría OCDE |
Bioquímica y Biología Molecular
Química
Multidisciplinar
|
| Materias |
microARN
estatina
hipercolesterolemia
enfermedades cardiovasculares
atorvastatina
COLESTEROL-LIPOPROTEÍNA DE DENSIDAD
ENFERMEDAD-ARTERIAL-CORONARIA
CARCINOMA-HEPATOCELULAR
ENFERMEDAD-CARDIOVASCULAR
SUJETOS CHILENOS
EXPRESIÓN
ESTATINAS
CÉLULAS
BIOMARCADORES
MIR-17-5P
|
| Título de la cita (Recomendado-único) |
MicroRNA-20a-5p Downregulation by Atorvastatin: A Potential Mechanism Involved in Lipid-Lowering Therapy
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
version publicada
|
| License |
CC BY 4.0
|
| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
|
| Access Rights |
acceso abierto
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID-FONDECYT 3160567
ANID-FONDECYT 1171765
UFRO DI19-0094
ANID 21181008
ANID FONDECYT 3160567
ANID FONDECYT 1171765
|
| Id de Web of Science |
WOS:000794627400001
|
cienciaabierta.ufro.cl
ciencia.abierta@ufrontera.cl