Repositorio UFRO · Omeka S

MicroRNA-20a-5p Downregulation by Atorvastatin: A Potential Mechanism Involved in Lipid-Lowering Therapy

Primer Autor	Salazar, Luis A.
Co-autores	Saavedra, Kathleen Leal, Karla Saavedra, Nicolas Prado, Yalena Paez, Isis Ubilla, Carmen G. Rojas, Gabriel
Título	MicroRNA-20a-5p Downregulation by Atorvastatin: A Potential Mechanism Involved in Lipid-Lowering Therapy
Editorial	MDPI
Revista	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lenguaje	en
Resumen	The treatment of hypercholesterolemia is mainly based on statins. However, the response to pharmacological therapy shows high inter-individual variability, resulting in variable effects in both lipid lowering and risk reduction. Thus, a better understanding of the lipid-lowering mechanisms and response variability at the molecular level is required. Previously, we demonstrated a deregulation of the microRNA expression profile in HepG2 cells treated for 24 h with atorvastatin, using a microarray platform. In the present study, we evaluated the expression of hsa-miR-17-5p, hsa-miR-20a-5p and hsa-miR-106a-5p in hypercholesterolemic patients before and after atorvastatin treatment and in HepG2 cells treated for 24 h with atorvastatin The miRNA hsa-mir-20a-5p was repressed after atorvastatin treatment in hypercholesteremic subjects and in HepG2 cells in culture. Repression of hsa-mir-20a-5p increased LDLR gene and protein expression in HepG2 cells, while hsa-mir-20a-5p overexpression reduced LDLR gene and protein expression.
Tipo de Recurso	artículo original
Description	This research was funded by Fondecyt-Chile (Grant numbers 3160567 and 1171765), Direccion de Investigacion of the Universidad de La Frontera (Grant number DI19-0094), and ANIDChile Doctoral Scholarship number 21181008.
doi	10.3390/ijms23095022
Formato Recurso	PDF
Palabras Claves	microRNA statin hypercholesterolemia cardiovascular diseases atorvastatin DENSITY-LIPOPROTEIN CHOLESTEROL CORONARY-ARTERY-DISEASE HEPATOCELLULAR-CARCINOMA CARDIOVASCULAR-DISEASE CHILEAN SUBJECTS EXPRESSION STATINS CELLS BIOMARKERS MIR-17-5P
Ubicación del archivo	http://dx.doi.org/10.3390/ijms23095022
Categoría OCDE	Bioquímica y Biología Molecular Química Multidisciplinar
Materias	microARN estatina hipercolesterolemia enfermedades cardiovasculares atorvastatina COLESTEROL-LIPOPROTEÍNA DE DENSIDAD ENFERMEDAD-ARTERIAL-CORONARIA CARCINOMA-HEPATOCELULAR ENFERMEDAD-CARDIOVASCULAR SUJETOS CHILENOS EXPRESIÓN ESTATINAS CÉLULAS BIOMARCADORES MIR-17-5P
Disciplinas de la OCDE	Endocrinología y Metabolismo (Incluye Diabetes, Hormonas) Farmacología y Farmacia Biología Molecular
Título de la cita (Recomendado-único)	MicroRNA-20a-5p Downregulation by Atorvastatin: A Potential Mechanism Involved in Lipid-Lowering Therapy
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	version publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID-FONDECYT 3160567 ANID-FONDECYT 1171765 UFRO DI19-0094 ANID 21181008 ANID FONDECYT 3160567 ANID FONDECYT 1171765
Id de Web of Science	WOS:000794627400001