Repositorio
Institucional

Bucchi, Cristina

Ohlsson, Ella

de Anta, Josep Maria

Woelflick, Melanie

Galler, Kerstin

Manzanares-Cespedes, Maria Cristina

Widbiller, Matthias

Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?

MDPI

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

en

The aim of this study was to analyze the suitability of pluripotent stem cells derived from the amnion (hAECs) as a potential cell source for revitalization in vitro. hAECs were isolated from human placentas, and dental pulp stem cells (hDPSCs) and dentin matrix proteins (eDMPs) were obtained from human teeth. Both hAECs and hDPSCs were cultured with 10% FBS, eDMPs and an osteogenic differentiation medium (StemPro). Viability was assessed by MTT and cell adherence to dentin was evaluated by scanning electron microscopy. Furthermore, the expression of mineralization-, odontogenic differentiation- and epithelial-mesenchymal transition-associated genes was analyzed by quantitative real-time PCR, and mineralization was evaluated through Alizarin Red staining. The viability of hAECs was significantly lower compared with hDPSCs in all groups and at all time points. Both hAECs and hDPSCs adhered to dentin and were homogeneously distributed. The regulation of odontoblast differentiation- and mineralization-associated genes showed the lack of transition of hAECs into an odontoblastic phenotype, however, genes associated with epithelial-mesenchymal transition were significantly upregulated in hAECs. hAECs showed small amounts of calcium deposition after osteogenic differentiation with StemPro. Pluripotent hAECs adhere on dentin and possess the capacity to mineralize. However, they presented an unfavorable proliferation behavior and failed to undergo odontoblastic transition.

artículo original

10.3390/ijms23052830

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human amnion epithelial cells

dental pulp stem cells

dentin matrix proteins

odontoblastic differentiation

revitalization

IMMATURE PERMANENT TEETH

MESENCHYMAL STEM-CELLS

OSTEOGENIC DIFFERENTIATION

GENE-EXPRESSION

HUMAN TOOTH

TGF-BETA

DENTIN

ENDODONTICS

GROWTH

TRANSPLANTATION

Bioquímica y Biología Molecular

Química

Multidisciplinar

células epiteliales del amnios humano

células madre de la pulpa dental

proteínas de la matriz de la dentina

diferenciación odontoblástica

revitalización

DIENTES PERMANENTES INMADUROS

CÉLULAS MADRE MESENQUIMALES

DIFERENCIACIÓN OSTEOGÉNICA

EXPRESIÓN GENÉTICA

DIENTE HUMANO

TGF-BETA

DENTINA

ENDODONCIA

CRECIMIENTO

TRASPLANTE

Odontología, Cirugía y Medicina Oral

Biología Celular

Biotecnología Relacionada con la Salud

Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?

artículo original

version publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

WOS:000768513900001