Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?

Primer Autor
Bucchi, Cristina
Co-autores
Ohlsson, Ella
de Anta, Josep Maria
Woelflick, Melanie
Galler, Kerstin
Manzanares-Cespedes, Maria Cristina
Widbiller, Matthias
Título
Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?
Editorial
MDPI
Revista
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lenguaje
en
Resumen
The aim of this study was to analyze the suitability of pluripotent stem cells derived from the amnion (hAECs) as a potential cell source for revitalization in vitro. hAECs were isolated from human placentas, and dental pulp stem cells (hDPSCs) and dentin matrix proteins (eDMPs) were obtained from human teeth. Both hAECs and hDPSCs were cultured with 10% FBS, eDMPs and an osteogenic differentiation medium (StemPro). Viability was assessed by MTT and cell adherence to dentin was evaluated by scanning electron microscopy. Furthermore, the expression of mineralization-, odontogenic differentiation- and epithelial-mesenchymal transition-associated genes was analyzed by quantitative real-time PCR, and mineralization was evaluated through Alizarin Red staining. The viability of hAECs was significantly lower compared with hDPSCs in all groups and at all time points. Both hAECs and hDPSCs adhered to dentin and were homogeneously distributed. The regulation of odontoblast differentiation- and mineralization-associated genes showed the lack of transition of hAECs into an odontoblastic phenotype, however, genes associated with epithelial-mesenchymal transition were significantly upregulated in hAECs. hAECs showed small amounts of calcium deposition after osteogenic differentiation with StemPro. Pluripotent hAECs adhere on dentin and possess the capacity to mineralize. However, they presented an unfavorable proliferation behavior and failed to undergo odontoblastic transition.
Tipo de Recurso
artículo original
doi
10.3390/ijms23052830
Formato Recurso
PDF
Palabras Claves
human amnion epithelial cells
dental pulp stem cells
dentin matrix proteins
odontoblastic differentiation
revitalization
IMMATURE PERMANENT TEETH
MESENCHYMAL STEM-CELLS
OSTEOGENIC DIFFERENTIATION
GENE-EXPRESSION
HUMAN TOOTH
TGF-BETA
DENTIN
ENDODONTICS
GROWTH
TRANSPLANTATION
Ubicación del archivo
http://dx.doi.org/10.3390/ijms23052830
Categoría OCDE
Bioquímica y Biología Molecular
Química
Multidisciplinar
Materias
células epiteliales del amnios humano
células madre de la pulpa dental
proteínas de la matriz de la dentina
diferenciación odontoblástica
revitalización
DIENTES PERMANENTES INMADUROS
CÉLULAS MADRE MESENQUIMALES
DIFERENCIACIÓN OSTEOGÉNICA
EXPRESIÓN GENÉTICA
DIENTE HUMANO
TGF-BETA
DENTINA
ENDODONCIA
CRECIMIENTO
TRASPLANTE
Disciplinas de la OCDE
Odontología, Cirugía y Medicina Oral
Biología Celular
Biotecnología Relacionada con la Salud
Título de la cita (Recomendado-único)
Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:000768513900001
Colecciones
Colección Publicaciones Científicas
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