Repositorio UFRO · Omeka S

Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?

Primer Autor	Bucchi, Cristina
Co-autores	Ohlsson, Ella de Anta, Josep Maria Woelflick, Melanie Galler, Kerstin Manzanares-Cespedes, Maria Cristina Widbiller, Matthias
Título	Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?
Editorial	MDPI
Revista	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lenguaje	en
Resumen	The aim of this study was to analyze the suitability of pluripotent stem cells derived from the amnion (hAECs) as a potential cell source for revitalization in vitro. hAECs were isolated from human placentas, and dental pulp stem cells (hDPSCs) and dentin matrix proteins (eDMPs) were obtained from human teeth. Both hAECs and hDPSCs were cultured with 10% FBS, eDMPs and an osteogenic differentiation medium (StemPro). Viability was assessed by MTT and cell adherence to dentin was evaluated by scanning electron microscopy. Furthermore, the expression of mineralization-, odontogenic differentiation- and epithelial-mesenchymal transition-associated genes was analyzed by quantitative real-time PCR, and mineralization was evaluated through Alizarin Red staining. The viability of hAECs was significantly lower compared with hDPSCs in all groups and at all time points. Both hAECs and hDPSCs adhered to dentin and were homogeneously distributed. The regulation of odontoblast differentiation- and mineralization-associated genes showed the lack of transition of hAECs into an odontoblastic phenotype, however, genes associated with epithelial-mesenchymal transition were significantly upregulated in hAECs. hAECs showed small amounts of calcium deposition after osteogenic differentiation with StemPro. Pluripotent hAECs adhere on dentin and possess the capacity to mineralize. However, they presented an unfavorable proliferation behavior and failed to undergo odontoblastic transition.
Tipo de Recurso	artículo original
doi	10.3390/ijms23052830
Formato Recurso	PDF
Palabras Claves	human amnion epithelial cells dental pulp stem cells dentin matrix proteins odontoblastic differentiation revitalization IMMATURE PERMANENT TEETH MESENCHYMAL STEM-CELLS OSTEOGENIC DIFFERENTIATION GENE-EXPRESSION HUMAN TOOTH TGF-BETA DENTIN ENDODONTICS GROWTH TRANSPLANTATION
Ubicación del archivo	http://dx.doi.org/10.3390/ijms23052830
Categoría OCDE	Bioquímica y Biología Molecular Química Multidisciplinar
Materias	células epiteliales del amnios humano células madre de la pulpa dental proteínas de la matriz de la dentina diferenciación odontoblástica revitalización DIENTES PERMANENTES INMADUROS CÉLULAS MADRE MESENQUIMALES DIFERENCIACIÓN OSTEOGÉNICA EXPRESIÓN GENÉTICA DIENTE HUMANO TGF-BETA DENTINA ENDODONCIA CRECIMIENTO TRASPLANTE
Disciplinas de la OCDE	Odontología, Cirugía y Medicina Oral Biología Celular Biotecnología Relacionada con la Salud
Versión del recurso (Recomendado-único)	version publicada
Tipo de ruta	dorada verde
Access Rights	acceso abierto
Derechos de acceso	acceso abierto
Id de Web of Science	WOS:000768513900001
Título de la cita (Recomendado-único)	Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?
Identificador del recurso (Mandatado-único)	artículo original
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0