Exosomal and Plasma Non-Coding RNA Signature Associated with Urinary Albumin Excretion in Hypertension

Primer Autor
Cortes, Raquel
Co-autores
Riffo-Campos, Angela L.
Perez-Hernandez, Javier
Ortega, Ana
Martinez-Arroyo, Olga
Flores-Chova, Ana
Redon, Josep
Título
Exosomal and Plasma Non-Coding RNA Signature Associated with Urinary Albumin Excretion in Hypertension
Editorial
MDPI
Revista
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lenguaje
en
Resumen
Non-coding RNA (ncRNA), released into circulation or packaged into exosomes, plays important roles in many biological processes in the kidney. The purpose of the present study is to identify a common ncRNA signature associated with early renal damage and its related molecular pathways. Three individual libraries (plasma and urinary exosomes, and total plasma) were prepared from each hypertensive patient (with or without albuminuria) for ncRNA sequencing analysis. Next, an RNA-based transcriptional regulatory network was constructed. The three RNA biotypes with the greatest number of differentially expressed transcripts were long-ncRNA (lncRNA), microRNA (miRNA) and piwi-interacting RNA (piRNAs). We identified a common 24 ncRNA molecular signature related to hypertension-associated urinary albumin excretion, of which lncRNAs were the most representative. In addition, the transcriptional regulatory network showed five lncRNAs (LINC02614, BAALC-AS1, FAM230B, LOC100505824 and LINC01484) and the miR-301a-3p to play a significant role in network organization and targeting critical pathways regulating filtration barrier integrity and tubule reabsorption. Our study found an ncRNA profile associated with albuminuria, independent of biofluid origin (urine or plasma, circulating or in exosomes) that identifies a handful of potential targets, which may be utilized to study mechanisms of albuminuria and cardiovascular damage.
Tipo de Recurso
artículo original
doi
10.3390/ijms23020823
Formato Recurso
PDF
Palabras Claves
urinary albumin excretion
hypertension
exosomes
plasma
non-coding RNA
PRACTICE GUIDELINES
KIDNEY
EXPRESSION
MICRORNAS
MECHANISMS
MANAGEMENT
CHANNELS
GENES
MIRNA
Ubicación del archivo
http://dx.doi.org/10.3390/ijms23020823
Categoría OCDE
Bioquímica y Biología Molecular
Química
Multidisciplinar
Materias
excreción de albúmina urinaria
hipertensión
exosomas
plasma
ARN no codificante
GUÍAS DE PRÁCTICA
RIÑÓN
EXPRESIÓN
MICRORNAS
MECANISMOS
GESTIÓN
CANALES
GENES
MIRNA
Disciplinas de la OCDE
Biología Molecular
Genética y Herencia
Urología y Nefrología
Título de la cita (Recomendado-único)
Exosomal and Plasma Non-Coding RNA Signature Associated with Urinary Albumin Excretion in Hypertension
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:000747827600001
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