Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis

Primer Autor
Sousa, Vanessa
Co-autores
Spratt, Dave
Davrandi, Mehmet
Mardas, Nikos
Beltran, Victor
Donos, Nikolaos
Título
Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis
Editorial
MDPI
Revista
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
Lenguaje
en
Resumen
Peri-implantitis is a disease influenced by dysbiotic microbial communities that play a role in the short- and long-term outcomes of its clinical treatment. The ecological triggers that establish the progression from peri-implant mucositis to peri-implantitis remain unknown. This investigation describes the development of a novel in vitro microcosm biofilm model. Biofilms were grown over 30 days over machined titanium discs in a constant depth film fermentor (CDFF), which was inoculated (I) with pooled human saliva. Following longitudinal biofilm sampling across peri-implant health (PH), peri-implant mucositis (PM), and peri-implantitis (PI) conditions, the characterisation of the biofilms was performed. The biofilm analyses included imaging by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), selective and non-selective culture media of viable biofilms, and 16S rRNA gene amplification and sequencing. Bacterial qualitative shifts were observed by CLSM and SEM across conditions, which were defined by characteristic phenotypes. A total of 9 phyla, 83 genera, and 156 species were identified throughout the experiment. The phyla Proteobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Actinobacteria showed the highest prevalence in PI conditions. This novel in vitro microcosm model provides a high-throughput alternative for growing microcosm biofilms resembling an in vitro progression from PH-PM-PI conditions.
Tipo de Recurso
artículo original
doi
10.3390/ijerph192114088
Formato Recurso
PDF
Palabras Claves
peri-implantitis
therapy
microcosm
oral biofilm
modelling biofilms
microbiome
microbial ecology
GINGIVAL CREVICE FLUID
DEPTH FILM FERMENTER
ANTIMICROBIAL SUSCEPTIBILITY
MICROBIOTA
BACTERIA
TITANIUM
SULCUS
MOUTH
PH
Ubicación del archivo
http://dx.doi.org/10.3390/ijerph192114088
Categoría OCDE
Ciencias Ambientales
Salud pública
ambiental y ocupacional
Materias
periimplantitis
terapia
microcosmos
biopelícula oral
biopelículas de modelado
microbioma
ecología microbiana
LÍQUIDO DE Grieta gingival
FERMENTADOR DE PELÍCULA PROFUNDA
SUSCEPTIBILIDAD ANTIMICROBIANA
MICROBIOTA
BACTERIAS
TITANIO
SULCUS
BOCA
PH
Disciplinas de la OCDE
Odontología, Cirugía y Medicina Oral
Microbiología
Biotecnología Relacionada con la Salud
Título de la cita (Recomendado-único)
Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:000881108300001
Colecciones
Colección Publicaciones Científicas
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