Repositorio UFRO · Omeka S

Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis

Primer Autor	Sousa, Vanessa
Co-autores	Spratt, Dave Davrandi, Mehmet Mardas, Nikos Beltran, Victor Donos, Nikolaos
Título	Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis
Editorial	MDPI
Revista	INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
Lenguaje	en
Resumen	Peri-implantitis is a disease influenced by dysbiotic microbial communities that play a role in the short- and long-term outcomes of its clinical treatment. The ecological triggers that establish the progression from peri-implant mucositis to peri-implantitis remain unknown. This investigation describes the development of a novel in vitro microcosm biofilm model. Biofilms were grown over 30 days over machined titanium discs in a constant depth film fermentor (CDFF), which was inoculated (I) with pooled human saliva. Following longitudinal biofilm sampling across peri-implant health (PH), peri-implant mucositis (PM), and peri-implantitis (PI) conditions, the characterisation of the biofilms was performed. The biofilm analyses included imaging by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), selective and non-selective culture media of viable biofilms, and 16S rRNA gene amplification and sequencing. Bacterial qualitative shifts were observed by CLSM and SEM across conditions, which were defined by characteristic phenotypes. A total of 9 phyla, 83 genera, and 156 species were identified throughout the experiment. The phyla Proteobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Actinobacteria showed the highest prevalence in PI conditions. This novel in vitro microcosm model provides a high-throughput alternative for growing microcosm biofilms resembling an in vitro progression from PH-PM-PI conditions.
Tipo de Recurso	artículo original
doi	10.3390/ijerph192114088
Formato Recurso	PDF
Palabras Claves	peri-implantitis therapy microcosm oral biofilm modelling biofilms microbiome microbial ecology GINGIVAL CREVICE FLUID DEPTH FILM FERMENTER ANTIMICROBIAL SUSCEPTIBILITY MICROBIOTA BACTERIA TITANIUM SULCUS MOUTH PH
Ubicación del archivo	http://dx.doi.org/10.3390/ijerph192114088
Categoría OCDE	Ciencias Ambientales Salud pública ambiental y ocupacional
Materias	periimplantitis terapia microcosmos biopelícula oral biopelículas de modelado microbioma ecología microbiana LÍQUIDO DE Grieta gingival FERMENTADOR DE PELÍCULA PROFUNDA SUSCEPTIBILIDAD ANTIMICROBIANA MICROBIOTA BACTERIAS TITANIO SULCUS BOCA PH
Disciplinas de la OCDE	Odontología, Cirugía y Medicina Oral Microbiología Biotecnología Relacionada con la Salud
Título de la cita (Recomendado-único)	Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	version publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:000881108300001