Repositorio
Institucional

Fuenzalida, Loreto F.

Arias-Bravo, Guisselle

Valderrama, Gustavo

Inostroza, Jaime

Tapia, Cecilia

Toro-Ascuy, Daniela

Ramilo, Octavio

Orellana, Paz

Cifuentes-Munoz, Nicolas

Zorondo-Rodriguez, Francisco

Mejias, Asuncion

Overnutrition, Nasopharyngeal Pathogenic Bacteria and Proinflammatory Cytokines in Infants with Viral Lower Respiratory Tract Infections

MDPI

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH

en

Background: Little is known about the interaction between the nasopharyngeal bacterial profile and the nutritional status in children. In this study, our main goal was to evaluate the associations between overnutrition and the presence of four potentially pathogenic bacteria in the nasopharynx of infants with viral lower respiratory tract infections (LRTI). In addition, we determined whether changes in the nasopharyngeal bacterial profile were associated with mucosal and serum proinflammatory cytokines and with clinical disease severity. Methods: We enrolled 116 children less than 2 years old hospitalized for viral LRTI during two consecutive respiratory seasons (May 2016 to August 2017), their nutritional status was assessed, and nasopharyngeal and blood samples were obtained. S. aureus, S. pneumoniae, H. influenzae, M. catarrhalis, and respiratory viruses were identified in nasopharyngeal samples by qPCR. Cytokine concentrations were measured in nasopharyngeal and blood samples. Disease severity was assessed by the length of hospitalization and oxygen therapy. Results: Nasopharyngeal pathogenic bacteria were identified in 96.6% of the enrolled children, and 80% of them tested positive for two or more bacteria. The presence and loads of M. catarrhalis was higher (p = 0.001 and p = 0.022, respectively) in children with overnutrition (n = 47) compared with those with normal weights (n = 69). In addition, the detection of >2 bacteria was more frequent in children with overnutrition compared to those with normal weight (p = 0.02). Multivariate regression models showed that the presence and loads of S. pneumoniae and M. catarrhalis were associated with higher concentrations of IL-6 in plasma and TNF-alpha in mucosal samples in children with overnutrition. Conclusions: The nasopharyngeal profile of young children with overnutrition was characterized by an over representation of pathogenic bacteria and proinflammatory cytokines.

artículo original

This research was funded by FONDECYT-INICIACION 11150599.

Esta investigación fue financiada por FONDECYT-INICIACION 11150599.

10.3390/ijerph19148781

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children

overnutrition

nasopharynx

pathogenic bacteria

viral respiratory infection

co-detection

STREPTOCOCCUS-PNEUMONIAE

DISEASE SEVERITY

VIRUSES

ASTHMA

COLONIZATION

MICROBIOTA

CHILDREN

MARKERS

AIRWAY

Ciencias Ambientales

Salud pública

ambiental y ocupacional

niños

sobrenutrición

nasofaringe

bacterias patógenas

infección respiratoria viral

codetección

STREPTOCOCCUS-PNEUMONIAE

SEVERIDAD DE LA ENFERMEDAD

VIRUS

ASMA

COLONIZACIÓN

MICROBIOTA

NIÑOS

MARCADORES

VÍA AÉREA

Overnutrition, Nasopharyngeal Pathogenic Bacteria and Proinflammatory Cytokines in Infants with Viral Lower Respiratory Tract Infections

artículo original

version publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

ANID-FONDECYT 11150599

ANID FONDECYT 11150599

WOS:000831993700001