On-Line Control of Feast/Famine Cycles to Improve PHB Accumulation during Cultivation of Mixed Microbial Cultures in Sequential Batch Reactors
| Primer Autor |
Jeison, David
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| Co-autores |
Cabrera, Francisco
Torres-Aravena, Alvaro
Pinto-Ibieta, Fernanda
Campos, Jose Luis,
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| Título |
On-Line Control of Feast/Famine Cycles to Improve PHB Accumulation during Cultivation of Mixed Microbial Cultures in Sequential Batch Reactors
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| Editorial |
MDPI
|
| Revista |
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
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| Lenguaje |
en
|
| Resumen |
Production of polyhydroxyalkanoates (PHA) has generated great interest as building blocks for bioplastic production. Their production using mixed microbial cultures represents an interesting alternative, since it enables the use of organic wastes as a carbon source. Feast/famine strategy is a common way to promote selection of microorganisms with PHA accumulation capacity. However, when using waste sources, changes in substrate concentration are expected, that may affect performance and efficiency of the process. This study showed how the dissolved oxygen level can be used for online control of the cycle time, ensuring that the desired feast/famine ratio is effectively applied. An operation strategy is presented and validated, using sequential batch reactors fed with acetate as the carbon source. Production of polyhydroxybutyrate (PHB) was studied, which is the expected type of PHA to be synthetized when using acetate as substrate. Two reactors were operated by applying the proposed control strategy, to provide F/F ratios of 0.2 and 0.6, respectively. A third reactor was operated with a fixed cycle time, for comparison purposes. Results showed that the reactor that operated at an F/F ratio of 0.6 promoted higher biomass productivity and PHB content, as a result of a better use of available time, preventing unnecessary long famine times. The application of the tested strategy is a simple a reliable way to promote a better performance of feast/famine-based bioreactors involving mixed microbial cultures for PHB production.
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| Tipo de Recurso |
artículo original
|
| Description |
This research was funded by CRHIAM Centre, grant number ANID/FONDAP/15130015. The APC was funded by Pontificia Universidad Catolica de Valparaiso.
Esta investigación fue financiada por el Centro CRHIAM, subvención número ANID/FONDAP/15130015. La APC fue financiada por la Pontificia Universidad Católica de Valparaíso.
|
| doi |
10.3390/ijerph182312611
|
| Formato Recurso |
PDF
|
| Palabras Claves |
PHB
bioplastics
SBR
dissolved oxygen
feast
famine
POLYHYDROXYALKANOATES PHAS PRODUCTION
RALSTONIA-EUTROPHA
ACTIVATED-SLUDGE
STRATEGIES
ENRICHMENT
SUBSTRATE
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| Ubicación del archivo | |
| Categoría OCDE |
Ciencias Ambientales
Salud pública
ambiental y ocupacional
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| Materias |
PHB
bioplásticos
SBR
oxígeno disuelto
fiesta
hambruna
PRODUCCIÓN DE POLIHIDROXIALCANANOATOS PHAS
RALSTONIA-EUTROPHA
LODOS-ACTIVADOS
ESTRATEGIAS
ENRIQUECIMIENTO
SUSTRATO
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| Título de la cita (Recomendado-único) |
On-Line Control of Feast/Famine Cycles to Improve PHB Accumulation during Cultivation of Mixed Microbial Cultures in Sequential Batch Reactors
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
version publicada
|
| License |
CC BY 4.0
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| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
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| Derechos de acceso |
acceso abierto
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| Access Rights |
acceso abierto
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID-FONDAP 15130015
ANID FONDAP 15130015
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| Id de Web of Science |
WOS:000734924900001
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