Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification function

Primer Autor
Mutis, Ana
Co-autores
Venthur, Herbert
Lizana, Paula
Manosalva, Loreto
Rojas, Valentina
Godoy, Ricardo
Rocha, Adonis
Aguilera, Ivan
Palma-Millanao, Ruben
Fajardo, Victor
Quiroz, Andres
Título
Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification function
Editorial
FRONTIERS MEDIA SA
Revista
FRONTIERS IN PHYSIOLOGY
Lenguaje
en
Resumen
The greater wax moth, Galleria mellonella, is a global pest for beehives, doing damage in the larval stage. Although a significant number of studies have reported on larvae and adults, to date no effective pest control has been implemented. In this study, we tested larval resistance to alkaloids from Berberis microphylla, and the objective was to identify enzymes that participate in alkaloid detoxification through enzymatic assays, bioinformatics analysis and qRT-PCR. Findings suggest glutathione-S-transferases (GSTs), from an increased metabolic mechanism, are responsible for alkaloid detoxification rather than cytochrome P450 (CYP), carboxylesterases (CarE). A bioinformatics analysis from transcriptome data revealed 22 GSTs present in both G. mellonella larvae and adults. The qRT-PCR experiments corroborated the presence of the 22 GSTs in larvae, where GST8 and GST20 stood out with the highest expression after berberine treatment. Structural information around GST8 and GST20 suggests that GST8 could bind berberine stronger than GST20. These findings represent an important advance in the study of detoxification enzymes in G. mellonella, expanding the role of delta-class GSTs towards alkaloids. Likewise, GST inhibition by alkaloid analogs is proposed in the framework of integrated pest management strategies.
Tipo de Recurso
artículo original
Description
This study was financially supported by the project DI19-2011 and partially supported by projects Fondecyt de Iniciacion 11200349 and DI21-1002.
Este estudio fue apoyado financieramente por el proyecto DI19-2011 y parcialmente apoyado por los proyectos Fondecyt de Iniciacion 11200349 y DI21-1002.
doi
10.3389/fphys.2022.989006
Formato Recurso
PDF
Palabras Claves
pest control
enzymatic activity
Galleria mellonella
alkaloids
glutathione-S-transferase (GST)
GREATER WAX MOTH
L. LEPIDOPTERA
RESISTANCE
GENERATION
BEHAVIOR
ENZYMES
Ubicación del archivo
http://dx.doi.org/10.3389/fphys.2022.989006
Categoría OCDE
Fisiología
Materias
control de plagas
actividad enzimática
Galleria mellonella
alcaloides
glutatión-S-transferasa (GST)
POLILLA GRANDE DE LA CERA
L. LEPIDOPTERA
RESISTENCIA
GENERACIÓN
COMPORTAMIENTO
ENZIMAS
Disciplinas de la OCDE
Zoología, Ornitología, Entomología y Comportamiento Biológico
Agricultura
Bioquímica
Título de la cita (Recomendado-único)
Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification function
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID-FONDECYT 11200349
UFRO DI19-2011
UFRO DI21-1002
ANID FONDECYT 11200349
Id de Web of Science
WOS:000860317400001
Colecciones
Colección Publicaciones Científicas
Colección ANID
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