Repositorio
Institucional

Castro-Varela, Pablo

Celis-Pla, Paula S. M.

Figueroa, Felix L.

Rubilar, Monica

Highly Efficient Water-Based Extraction of Biliprotein R-Phycoerythrin From Marine the Red-Macroalga Sarcopeltis skottsbergii by Ultrasound and High-Pressure Homogenization Methods

FRONTIERS MEDIA SA

FRONTIERS IN MARINE SCIENCE

en

Sarcopeltis skottsbergii is an endemic species of the southern region of South America, with R-phycoerythrin (R-PE) as an accessory photosynthetic pigment. The production of S. skottsbergii is around twenty thousand tons of dry alga per year. The evaluation of (R-PE) in a biorefinery model is still incipient in the algal biotechnology area and will be used in the food, pharmaceutical, cosmeceutical, and nutraceutical industries. This work evaluated the cell disruption and separation processes by using two green technologies, ultrasound-assisted extraction (UAE) and high-pressure homogenization (HPH), to obtain an R-phycoerythrin enriched extract from S. skottsbergii. Two-levels three-factor central composite design (CCD) and response surface methodology (RSM) were carried out to optimize the extraction conditions, including the factors for UAE (time, amplitude, and solvent) and HPH (Pressure, number of passes, and solvent). Additionally, a second-order polynomial fit was performed to fit the experimental data by the green method. HPH method was the most efficient extraction method under the conditions obtained of 100-400 MPa pressure power, 2-3 number of passes, and distilled water as solvent. Furthermore, the experimental extraction yields ranged from 4.4-5.7 mg of PE g(-1) of dry biomass under the optimal extraction conditions (400 MPa, 2 passes), which agreed with the predictive yield of 4.6-5.5 mg g(-1) DW. The ultrafiltration membrane used for the separation process for both methods exhibited a rejection of R-phycoerythrin concentrated at 30 KDa. Furthermore, R-phycoerythrin showed a positive correlation between the antioxidant capacity (ORAC) in the best-selected extractions. After the extraction, the same pattern was observed in Chlorophyll a and total carotenoids with DPPH. Thus, it was an attractive non-aggressive extraction alternative with biological activity of interest for formulating biotechnological products for the food industry is suggested.

artículo original

The research leading to these results has received funding from National Agency of Development and Research of Chile (PhD Scholarship No21180257) and Andalusian government, Spain (Project FACCO, UMA18-FEDER JA-162).

La investigación que condujo a estos resultados ha recibido financiación de la Agencia Nacional de Desarrollo e Investigación de Chile (Beca de Doctorado No21180257) y del Gobierno de Andalucía, España (Proyecto FACCO, UMA18-FEDER JA-162).

10.3389/fmars.2022.877177

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R-phycoerythrin

Sarcopeltis skottsbergii

ultrasound

high-pressure homogenization

red algae

ASSISTED EXTRACTION

GRATELOUPIA-TURUTURU

B-PHYCOERYTHRIN

PURIFICATION

PROTEIN

ANTIOXIDANT

FOOD

PHYCOBILIPROTEINS

RECOVERY

SEAWEEDS

Ciencias Ambientales

Biología marina y de agua dulce

R-ficoeritrina

Sarcopeltis skottsbergii

ultrasonido

homogeneización a alta presión

algas rojas

EXTRACCIÓN ASISTIDA

GRATELOUPIA-TURUTURU

B-FICOERITRINA

PURIFICACIÓN

PROTEÍNA

ANTIOXIDANTE

ALIMENTO

FICOBILIPROTEÍNAS

RECUPERACIÓN

ALGAS MARINAS

Biotecnología Industrial

Otras Especialidades de la Biología

Bioproductos

Highly Efficient Water-Based Extraction of Biliprotein R-Phycoerythrin From Marine the Red-Macroalga Sarcopeltis skottsbergii by Ultrasound and High-Pressure Homogenization Methods

artículo original

version publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

ANID 21180257

JA UMA18-FEDER JA-162

WOS:000819356500001