Genome-Wide Identification of Aldehyde Oxidase Genes in Moths and Butterflies Suggests New Insights Into Their Function as Odorant-Degrading Enzymes

Primer Autor
Venthur, Herbert
Co-autores
Godoy, Ricardo
Mutis, Ana
Carabajal Paladino, Leonela
Título
Genome-Wide Identification of Aldehyde Oxidase Genes in Moths and Butterflies Suggests New Insights Into Their Function as Odorant-Degrading Enzymes
Editorial
FRONTIERS MEDIA SA
Revista
FRONTIERS IN ECOLOGY AND EVOLUTION
Lenguaje
en
Resumen
Aldehyde oxidases (AOXs) are common detoxifying enzymes in several organisms. In insects, AOXs act in xenobiotic metabolism and as odorant-degrading enzymes (ODEs). These last appear as crucial enzymes in the life cycle of insects, helping to reset their olfactory system, particularly in lepidopterans, which fulfill important ecological roles (e.g., pollination or destructive life cycles). A comprehensive understanding of their olfactory system has provided opportunities to study key chemosensory proteins. However, no significant advance has been made around lepidopteran AOXs research, and even less around butterflies, a recently evolved lineage. In this study we have identified novel AOX gene families in moths and butterflies in order to understand their role as ODEs. Eighteen genomes from both moths and butterflies were used for phylogenetics, molecular evolution and sequence analyses. We identified 164 AOXs, from which 91 are new. Their phylogeny showed two main clades that are potentially related to odorant-degrading function, where both moths and butterflies have AOXs. A first ODE-related clade seems to have a non-ditrysian origin, likely related to plant volatiles. A second ODE-related clade could be more pheromone-biased. Molecular evolution analysis suggests a slight purifying selection process, though a number of sites appeared under positive selection. ODE-related AOXs have changed a phenylalanine residue by proline in the active site. Finally, this study could serve as a reference for further evolutionary and functional studies around Lepidopteran AOXs.
Tipo de Recurso
artículo original
doi
10.3389/fevo.2022.823119
Formato Recurso
PDF
Palabras Claves
Lepidoptera
insect olfaction
aldehyde oxidase
genome
phylogenetics
MULTIPLE SEQUENCE ALIGNMENT
CODON-SUBSTITUTION MODELS
SEX-PHEROMONE
BOMBYX-MORI
BINDING-PROTEINS
LEPIDOPTERA
ANTENNAE
EVOLUTION
CARBOXYLESTERASE
SPECIFICITIES
Ubicación del archivo
http://dx.doi.org/10.3389/fevo.2022.823119
Categoría OCDE
Ecología
Materias
Lepidoptera
olfato de insectos
aldehído oxidasa
genoma
filogenética
ALINEACIÓN DE SECUENCIAS MÚLTIPLES
MODELOS DE SUSTITUCIÓN DE CODONES
FEROMONAS SEXUALES
BOMBYX-MORI
PROTEÍNAS DE UNIÓN
LEPIDOPTERA
ANTENAS
EVOLUCIÓN
CARBOXILESTERASA
ESPECIFICIDADES
Disciplinas de la OCDE
Zoología, Ornitología, Entomología y Comportamiento Biológico
Genética y Herencia
Biología Molecular
Título de la cita (Recomendado-único)
Genome-Wide Identification of Aldehyde Oxidase Genes in Moths and Butterflies Suggests New Insights Into Their Function as Odorant-Degrading Enzymes
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:000760595700001
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