Repositorio
Institucional

Braz-Silva, Paulo Henrique

Pallos, Debora

Sousa, Vanessa

Feres, Magda

Retamal-Valdes, Belen

Chen, Tsute

Curtis, Mike

Boaventura, Richardson Mondego

Tanaka, Marcia Hiromi

Salomao, Gustavo Vargas da Silva

Zanella, Louise

Tozetto-Mendoza, Tania Regina

Schwab, Gabriela

Franco, Lucas Augusto Moyses

Sabino, Ester Cerdeira

Shibli, Jamil Awad

Salivary Microbial Dysbiosis Is Associated With Peri-Implantitis: A Case-Control Study in a Brazilian Population

FRONTIERS MEDIA SA

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

en

Background and ObjectivesThe aim of this study was to examine the salivary microbiome in healthy peri-implant sites and those with peri-implantitis. MethodsSaliva samples were collected from 21 participants with healthy peri-implant sites and 21 participants with peri-implantitis. The V4 hypervariable region of the 16S rRNA gene was sequenced using the Ion Torrent PGM System (Ion 318 (TM) Chip v2 400). The NGS analysis and composition of the salivary microbiome were determined by taxonomy assignment. Downstream bioinformatic analyses were performed in QIIME (v 1.9.1). ResultsClinical differences according to peri-implant condition status were found. Alpha diversity metrics revealed that the bacterial communities of participants with healthy peri-implant sites tended to have a richer microbial composition than individuals with peri-implantitis. In terms of beta diversity, bleeding on probing (BoP) may influence the microbial diversity. However, no clear partitioning was noted between the salivary microbiome of volunteers with healthy peri-implant sites or volunteers with peri-implantitis. The highest relative abundance of Stenotrophomonas, Enterococcus and Leuconostoc genus, and Faecalibacterium prausnitzii, Haemophilus parainfluenzae, Prevotella copri, Bacteroides vulgatus, and Bacteroides stercoris bacterial species was found in participants with peri-implantitis when compared with those with healthy peri-implant sites. ConclusionDifferences in salivary microbiome composition were observed between patients with healthy peri-implant sites and those with peri-implantitis. BoP could affect the diversity (beta diversity) of the salivary microbiome.

artículo original

This study was supported by grants from the Sao Paulo Research Foundation (FAPESP)-grant numbers: 2013/08242-3 and 2015/07727-9, Brazilian Coordination for the Improvement of Higher Education Personnel (CAPES)-financial code 001, National Council for Scientific and Technological Development (CNPq)-grant numbers: 311368/2019-0 and 44004/2014-5, National Institute of Health Research (NIHR CL), National Funding for Scientific and Technologic Development of Chile (FONDECYT)-grant number: 3210687, Universidad de La Frontera (DIUFRO)-grant number: DIM20-0019, and Universidad de La Frontera-grant number: VRIP20P002.

Este estudio fue apoyado por subvenciones de la Fundación de Investigación de São Paulo (FAPESP): números de subvención: 2013/08242-3 y 2015/07727-9, Coordinación Brasileña de Perfeccionamiento del Personal de Educación Superior (CAPES) - código financiero 001, Consejo Nacional de Desarrollo Científico y Tecnológico (CNPq) - números de concesión: 311368/2019-0 y 44004/2014-5, Instituto Nacional de Investigaciones en Salud (NIHR CL), Fondo Nacional para el Desarrollo Científico y Tecnológico de Chile (FONDECYT) -número de concesión: 3210687, Universidad de La Frontera (DIUFRO)-número de subvención: DIM20-0019, y Universidad de La Frontera-número de subvención: VRIP20P002.

This study was supported by grants from the Sao Paulo Research Foundation (FAPESP)-grant numbers: 2013/08242-3 and 2015/07727-9; Brazilian Coordination for the Improvement of Higher Education Personnel (CAPES)-financial code 001; National Council for Scientific and Technological Development (CNPq)-grant numbers: 311368/2019-0 and 44004/2014-5; National Institute of Health Research (NIHR CL); National Funding for Scientific and Technologic Development of Chile (FONDECYT)-grant number: 3210687; Universidad de La Frontera (DIUFRO)-grant number: DIM20-0019; and Universidad de La Frontera-grant number: VRIP20P002.

Este estudio fue apoyado por subvenciones de la Fundación de Investigación de São Paulo (FAPESP): números de subvención: 2013/08242-3 y 2015/07727-9; Coordinación Brasileña de Perfeccionamiento del Personal de Educación Superior (CAPES) - código financiero 001; Consejo Nacional de Desarrollo Científico y Tecnológico (CNPq) - números de concesión: 311368/2019-0 y 44004/2014-5; Instituto Nacional de Investigaciones en Salud (NIHR CL); Fondo Nacional para el Desarrollo Científico y Tecnológico de Chile (FONDECYT) -número de concesión: 3210687; Universidad de La Frontera (DIUFRO)-número de subvención: DIM20-0019; y Universidad de La Frontera-número de subvención: VRIP20P002.

10.3389/fcimb.2021.696432

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peri-implantitis

saliva

microbiota (16S)

host-bacteria interaction

dysbioses

BIOFILM

HEALTHY

SUPRAGINGIVAL

DIVERSITY

SITES

Inmunología

Microbiología

periimplantitis

saliva

microbiota (16S)

interacción huésped-bacteria

disbiosis

BIOFILM

SALUD

SUPRAGINGIVAL

DIVERSIDAD

SITIOS

Microbiología

Odontología, Cirugía y Medicina Oral

Biotecnología Relacionada con la Salud

Salivary Microbial Dysbiosis Is Associated With Peri-Implantitis: A Case-Control Study in a Brazilian Population

artículo original

version publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

FAPESP 2013/08242-3

FAPESP 2015/07727-9

CNPQ 311368/2019-0

CNPQ 44004/2014-5

ANID-FONDECYT 3210687

UFRO DIM20-0019

UFRO VRIP20P002

ANID FONDECYT 3210687

WOS:000745786400001