Repositorio
Institucional

Salazar, Luis A.

Arencibia, Alberto

Salazar, Luis A.

Microarray meta-analysis reveals IL6 and p38 beta/MAPK11 as potential targets of hsa-miR-124 in endothelial progenitor cells: Implications for stent re-endothelization in diabetic patients

FRONTIERS MEDIA SA

FRONTIERS IN CARDIOVASCULAR MEDICINE

en

"Circulating endothelial progenitor cells (EPCs) play an important role in the repair processes of damaged vessels, favoring re-endothelization of stented vessels to minimize restenosis. EPCs number and function is diminished in patients with type 2 diabetes, a known risk factor for restenosis. Considering the impact of EPCs in vascular injury repair, we conducted a meta-analysis of microarray to assess the transcriptomic profile and determine target genes during the differentiation process of EPCs into mature ECs. Five microarray datasets, including 13 EPC and 12 EC samples were analyzed, using the online tool ExpressAnalyst. Differentially expressed genes (DEGs) analysis was done by Limma method, with an log(2)FC > 1 and FDR < 0.05. Combined p-value by Fisher exact method was computed for the intersection of datasets. There were 3,267 DEGs, 1,539 up-regulated and 1,728 down-regulated in EPCs, with 407 common DEGs in at least four datasets. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed enrichment for terms related to ""AGE-RAGE signaling pathway in diabetic complications."" Intersection of common DEGs, KEGG pathways genes and genes in protein-protein interaction network (PPI) identified four key genes, two up-regulated (IL1B and STAT5A) and two down-regulated (IL6 and MAPK11). MicroRNA enrichment analysis of common DEGs depicted five hub microRNA targeting 175 DEGs, including STAT5A, IL6 and MAPK11, with hsa-miR-124 as common regulator. This group of genes and microRNAs could serve as biomarkers of EPCs differentiation during coronary stenting as well as potential therapeutic targets to improve stent re-endothelization, especially in diabetic patients."

artículo original

Funding This research was supported by FONDECYT-ANID, Chile (Grant no. 1171765), the Direccion de Investigacion-Universidad de La Frontera, Temuco, Chile (Grant no. DI20-0092), and the CONICYT Scholarship (Grant no. 63140032).

10.3389/fcvm.2022.964721

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circulating progenitor endothelial cells

stent restenosis

diabetes

microarray meta-analysis

micro-RNA

RESTENOSIS

ATHEROSCLEROSIS

MECHANISMS

INCREASE

PATHWAY

Sistemas cardíacos y cardiovasculares

células endoteliales progenitoras circulantes

reestenosis del stent

diabetes

metanálisis de microarrays

microARN

RESTENOSIS

ATEROSCLEROSIS

MECANISMOS

AUMENTO

CAMINO

Sistema Cardiovascular y Cardiaco

Biotecnología Relacionada con la Salud

Endocrinología y Metabolismo (Incluye Diabetes, Hormonas)

Microarray meta-analysis reveals IL6 and p38 beta/MAPK11 as potential targets of hsa-miR-124 in endothelial progenitor cells: Implications for stent re-endothelization in diabetic patients

artículo original

version publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

ANID-FONDECYT 1171765

CONICYT 63140032

UFRO DI20-0092

ANID FONDECYT 1171765

WOS:000861085700001