Repositorio
Institucional

Almonacid-Munoz, Leonardo

Herrera, Hector

Fuentes-Ramirez, Andres

Vargas-Gaete, Rodrigo

Larama, Giovanni

Jara, Ronald

Fernandez-Urrutia, Camila

da Silva Valadares, Rafael Borges

Tree Cover Species Modify the Diversity of Rhizosphere-Associated Microorganisms in Nothofagus obliqua (Mirb.) Oerst Temperate Forests in South-Central Chile

MDPI

FORESTS

en

Chilean native forests have been subjected to several types of disturbances, with one of them being the replacement by exotic species. Pinus radiata D. Don is a widespread exotic tree that forms extensive plantations in southern Chile. It covers extended areas, affecting the landscape, biodiversity, and ecosystem services associated with native forest ecosystems. Although advances in assessing the impact of exotic plant species have been conducted, few studies have focused on the alteration of soil microorganisms. This study aimed to characterize the rhizosphere bacterial and fungal communities associated with the tree species Nothofagus obliqua inside a native forest stand and within a P. radiata plantation growing nearby. We used a 16S rRNA gene and ITS region metabarcoding approach. Using bioinformatics, diversity indices, relative abundance, preferential taxa, and predicted functions and guilds were estimated. The beta-diversity analysis showed that both factors, the type of soil (rhizosphere or bulk soil) and the type of site (native forest or P. radiata plantation), were significant, with the site explaining most of the variation among bacterial and fungal communities. Proteobacteria and Basidiomycota were the most abundant bacterial and fungal phyla in both types of soil and sites. Similarly, bacteria showed similar abundant taxa at the family level, independent of the soil type or the site. The main fungal taxa associated with native forests were Tricholomataceae and Cantharellales, whereas in P. radiata plantations, Russulaceae and Hyaloscyphaceae were the most abundant families. The main bacteria functional groups were chemoheterotrophy and aerobic chemoheterotrophy, without significant differences between the type of soil or sites. Overall, these results demonstrate that the composition and diversity of bacterial and fungal communities associated with native N. obliqua forest are influenced by the surrounding forest, and mainly depend on the site's characteristics, such as the lignin-rich wood source. These results improve our understanding of the impact of native forest replacement on soil microbial communities, which can alter microbial-related soil ecosystem services.

artículo original

The authors are thankful for the supercomputing infrastructure of Soroban (SATREPS MACH-JPM/JSA1705) at Centro de Modelacion y Computacion Cientifica at Universidad de La Frontera, and the Parque Ecologico y Cultural Rucamanque staff for technical support. H.H. is thankful for the financial support of the Fondo Nacional de Desarrollo Cientifico y Tecnologico, grant number 3200134. A.F.R. thanks ANID BASAL FB210015 (CENAMAD) and is thankful for the support received from grant ANID SCIA-Anillo ACT210052, and R.V.G. thanks the funding received by:ANID BASAL FB210015 (CENAMAD) and DIUFRO DI22-0042.

Los autores agradecen la infraestructura de supercomputación de Soroban (SATREPS MACH-JPM/JSA1705) en el Centro de Modelación y Computación Científica de la Universidad de La Frontera, y al personal del Parque Ecológico y Cultural Rucamanque por el soporte técnico. S.H. agradece el apoyo financiero del Fondo Nacional de Desarrollo Científico y Tecnológico, subvención número 3200134. A.F.R. agradece a ANID BASAL FB210015 (CENAMAD) y agradece el apoyo recibido del subsidio ANID SCIA-Anillo ACT210052, y R.V.G. agradece el financiamiento recibido por:ANID BASAL FB210015 (CENAMAD) y DIUFRO DI22-0042.

10.3390/f13050756

PDF

bacteria

fungi

native forest

pine plantation

rhizosphere

soil microbial communities

MICROBIAL COMMUNITIES

EXOTIC PLANTATIONS

SOIL

IDENTIFICATION

RESPONSES

BACTERIA

DATABASE

STRESS

Silvicultura

bacterias

hongos

bosque nativo

plantación de pinos

rizosfera

comunidades microbianas del suelo

COMUNIDADES MICROBIANAS

PLANTACIONES EXÓTICAS

SUELO

IDENTIFICACIÓN

RESPUESTAS

BACTERIAS

BASE DE DATOS

ESTRÉS

Tree Cover Species Modify the Diversity of Rhizosphere-Associated Microorganisms in Nothofagus obliqua (Mirb.) Oerst Temperate Forests in South-Central Chile

artículo original

version publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

ANID-FONDECYT 3200134

ANID-BASAL FB210015

ANID ACT210052

UFRO DI22-0042

ANID FONDECYT 3200134

ANID BASAL FB210015

WOS:000801835000001