Use of Anthracophyllum discolor and Stereum hirsutum as a Suitable Strategy for Delignification and Phenolic Removal of Olive Mill Solid Waste

Primer Autor
Ciudad, Gustavo
Co-autores
Benavides, Viviana
Pinto-Ibieta, Fernanda
Serrano, Antonio
Rubilar, Olga
Título
Use of Anthracophyllum discolor and Stereum hirsutum as a Suitable Strategy for Delignification and Phenolic Removal of Olive Mill Solid Waste
Editorial
MDPI
Revista
FOODS
Lenguaje
en
Resumen
This study evaluated the use of the white-rot fungi (WRF) Anthracophyllum discolor and Stereum hirsutum as a biological pretreatment for olive mill solid mill waste (OMSW). The WRF strains proposed were added directly to OMSW. The assays consisted of determining the need to add supplementary nutrients, an exogenous carbon source or use agitation systems, and evaluating WRF growth, enzyme activity, phenolic compound removal and lignin degradation. The highest ligninolytic enzyme activity was found at day 10, reaching 176.7 U/L of manganese-independent peroxidase (MniP) produced by A. discolor, and the highest phenolic removal (more than 80% with both strains) was reached after 24 days of incubation. The confocal laser scanning microscopy analysis (CLSM) confirmed lignin degradation through the drop in lignin relative fluorescence units (RFU) from 3967 for untreated OMSW to 235 and 221 RFU, showing a lignin relative degradation of 94.1% and 94.4% after 24 days of treatment by A. discolor and S. hirsutum, respectively. The results demonstrate for the first time that A. discolor and S. hirsutum were able to degrade lignin and remove phenolic compounds from OMSW using this as the sole substrate without adding other nutrients or using agitation systems. This work indicates that it could be possible to design an in situ pretreatment of the valorization of OMSW, avoiding complex systems or transportation. In this sense, future research under non-sterile conditions is needed to evaluate the competition of WRF with other microorganisms present in the OMSW. The main drawbacks of this work are associated with both the low reaction time and the water addition. However, OMSW is seasonal waste produced in one season per year, being stored for a long time. In terms of water addition, the necessary optimization will be addressed in future research.
Tipo de Recurso
artículo original
Description
Fondecyt project No. 1191230, Agencia Nacional de Investigacion y Desarrollo de Chile, ANID.
doi
10.3390/foods11111587
Formato Recurso
PDF
Palabras Claves
white-rot fungi
OMSW
ligninolytic enzyme
pretreatment
agroindustrial waste valorization
WHITE-ROT FUNGI
PHANEROCHAETE-CHRYSOSPORIUM
MANGANESE PEROXIDASE
LIGNINOLYTIC ENZYMES
WHEAT-STRAW
STATE FERMENTATION
CULTURE-CONDITIONS
PRETREATMENT
DETOXIFICATION
DEGRADATION
Ubicación del archivo
http://dx.doi.org/10.3390/foods11111587
Categoría OCDE
Ciencia y tecnología de los alimentos
Materias
hongos de pudrición blanca
OMSW
enzima ligninolítica
pretratamiento
valorización de residuos agroindustriales
HONGOS DE PUDRE BLANCA
PHANEROCHAETE-CHRYSOSPORIUM
PEROXIDASA DE MANGANESO
ENZIMAS LIGNINOLÍTICAS
PAJA DE TRIGO
ESTADO DE FERMENTACIÓN
CONDICIONES DE CULTIVO
PRETRATAMIENTO
DESTOXIFICACIÓN
DEGRADACIÓN
Disciplinas de la OCDE
Biorremediación
Biotecnología Ambiental
Micología
Título de la cita (Recomendado-único)
Use of Anthracophyllum discolor and Stereum hirsutum as a Suitable Strategy for Delignification and Phenolic Removal of Olive Mill Solid Waste
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID-FONDECYT 1191230
ANID FONDECYT 1191230
Id de Web of Science
WOS:000808817800001
Colecciones
Colección Publicaciones Científicas
Colección ANID
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