Repositorio
Institucional

Ciudad, Gustavo

Benavides, Viviana

Pinto-Ibieta, Fernanda

Serrano, Antonio

Rubilar, Olga

Use of Anthracophyllum discolor and Stereum hirsutum as a Suitable Strategy for Delignification and Phenolic Removal of Olive Mill Solid Waste

MDPI

FOODS

en

This study evaluated the use of the white-rot fungi (WRF) Anthracophyllum discolor and Stereum hirsutum as a biological pretreatment for olive mill solid mill waste (OMSW). The WRF strains proposed were added directly to OMSW. The assays consisted of determining the need to add supplementary nutrients, an exogenous carbon source or use agitation systems, and evaluating WRF growth, enzyme activity, phenolic compound removal and lignin degradation. The highest ligninolytic enzyme activity was found at day 10, reaching 176.7 U/L of manganese-independent peroxidase (MniP) produced by A. discolor, and the highest phenolic removal (more than 80% with both strains) was reached after 24 days of incubation. The confocal laser scanning microscopy analysis (CLSM) confirmed lignin degradation through the drop in lignin relative fluorescence units (RFU) from 3967 for untreated OMSW to 235 and 221 RFU, showing a lignin relative degradation of 94.1% and 94.4% after 24 days of treatment by A. discolor and S. hirsutum, respectively. The results demonstrate for the first time that A. discolor and S. hirsutum were able to degrade lignin and remove phenolic compounds from OMSW using this as the sole substrate without adding other nutrients or using agitation systems. This work indicates that it could be possible to design an in situ pretreatment of the valorization of OMSW, avoiding complex systems or transportation. In this sense, future research under non-sterile conditions is needed to evaluate the competition of WRF with other microorganisms present in the OMSW. The main drawbacks of this work are associated with both the low reaction time and the water addition. However, OMSW is seasonal waste produced in one season per year, being stored for a long time. In terms of water addition, the necessary optimization will be addressed in future research.

artículo original

Fondecyt project No. 1191230, Agencia Nacional de Investigacion y Desarrollo de Chile, ANID.

10.3390/foods11111587

PDF

white-rot fungi

OMSW

ligninolytic enzyme

pretreatment

agroindustrial waste valorization

WHITE-ROT FUNGI

PHANEROCHAETE-CHRYSOSPORIUM

MANGANESE PEROXIDASE

LIGNINOLYTIC ENZYMES

WHEAT-STRAW

STATE FERMENTATION

CULTURE-CONDITIONS

PRETREATMENT

DETOXIFICATION

DEGRADATION

Ciencia y tecnología de los alimentos

hongos de pudrición blanca

OMSW

enzima ligninolítica

pretratamiento

valorización de residuos agroindustriales

HONGOS DE PUDRE BLANCA

PHANEROCHAETE-CHRYSOSPORIUM

PEROXIDASA DE MANGANESO

ENZIMAS LIGNINOLÍTICAS

PAJA DE TRIGO

ESTADO DE FERMENTACIÓN

CONDICIONES DE CULTIVO

PRETRATAMIENTO

DESTOXIFICACIÓN

DEGRADACIÓN

Biorremediación

Biotecnología Ambiental

Micología

Use of Anthracophyllum discolor and Stereum hirsutum as a Suitable Strategy for Delignification and Phenolic Removal of Olive Mill Solid Waste

artículo original

version publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

ANID-FONDECYT 1191230

ANID FONDECYT 1191230

WOS:000808817800001