Seminal plasma, and not sperm, induces time and concentration-dependent neutrophil extracellular trap release in donkeys

Primer Autor
Miro, Jordi
Co-autores
Mateo-Otero, Yentel
Zambrano, Fabiola
Catalan, Jaime
Sanchez, Raul
Yeste, Marc
Fernandez-Fuertes, Beatriz
Título
Seminal plasma, and not sperm, induces time and concentration-dependent neutrophil extracellular trap release in donkeys
Editorial
WILEY
Revista
EQUINE VETERINARY JOURNAL
Lenguaje
en
Resumen
Background In several mammalian species, acute endometritis driven by the recruitment of polymorphonuclear cells (PMN) occurs in response to semen. These PMNs release DNA to form neutrophil extracellular traps (NETs) in cattle, horse and human, leading to sperm entrapment. While there is no evidence of this phenomenon occurring in donkeys, artificial insemination (AI) with frozen-thawed semen, which results in very poor pregnancy rates, leads to a large PMN recruitment to the uterus. Objectives To investigate whether donkey semen can trigger NET release (NETosis) and if excessive NETosis occurs in response to frozen-thawed semen. Study design In vitro experiments. Methods Jenny PMNs were exposed to jackass fresh or frozen-thawed semen, isolated sperm or seminal plasma (SP), over the course of three experiments. NET formation in response to different treatments was assessed through manual quantification of stained slides. A one-way analysis of variance (ANOVA), followed by a post hoc Sidak test, was carried out to determine statistical significance. Results NET release occurred in a semen concentration- and incubation-time-dependent manner. Surprisingly, frozen-thawed donkey sperm did not increase NETosis rate in comparison with the control (23 +/- 2.5% vs. 31 +/- 3.7%, P > .05), whereas fresh semen exposure did (78 +/- 5.7% vs. 26 +/- 3.2%, P < .01). NETosis increased in the presence of SP, regardless of the presence or absence of sperm, in comparison with the control in both fresh (84 +/- 5.2% and 77 +/- 5.0% vs. 12 +/- 2.7%, respectively, P < .01) and frozen (95 +/- 2.2% and 94 +/- 2.9% vs. 14 +/- 3.8%, respectively, P < .01) samples. Moreover, exposure of PMN to viable and motile sperm, in the absence of SP, did not increase NETosis rates (P > .05). Conclusions Donkey SP, and not sperm-intrinsic factors, is able to trigger NETosis in both time- and semen concentration-dependent manner. The physiological relevance of such response against semen in the donkey remains to be elucidated.
Tipo de Recurso
artículo original
doi
10.1111/evj.13457
Formato Recurso
PDF
Palabras Claves
donkey
horse
NETosis
seminal plasma
PMN
INFLAMMATORY RESPONSE
SEMEN
FERTILITY
RECRUITMENT
LEUKOCYTES
JENNIES
BINDING
Ubicación del archivo
http://dx.doi.org/10.1111/evj.13457
Categoría OCDE
Ciencias Veterinarias
Materias
burro
caballo
NETosis
plasma seminal
PMN
RESPUESTA INFLAMATORIA
SEMEN
FERTILIDAD
RECLUTAMIENTO
LEUCOCITOS
JENNIES
UNIÓN
Disciplinas de la OCDE
Ciencias Veterinarias
Biología Reproductiva
Biología Celular
Título de la cita (Recomendado-único)
Seminal plasma, and not sperm, induces time and concentration-dependent neutrophil extracellular trap release in donkeys
Página de inicio (Recomendado-único)
415
Página final (Recomendado-único)
426
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
Condición de la licencia (Recomendado-repetible)
0
Derechos de acceso
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Access Rights
metadata
Id de Web of Science
WOS:000656702900001
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