Repositorio
Institucional

Reyes-Diaz, Marjorie M.

Gonzalez-Villagra, Jorge

Omena-Garcia, Rebeca Patricia

Rodrigues-Salvador, Acacio

Nunes-Nesi, Adriano

Cohen, Jerry D.

Differential physiological and metabolic responses in young and fully expanded leaves of Aristotelia chilensis plants subjected to drought stress

PERGAMON-ELSEVIER SCIENCE LTD

ENVIRONMENTAL AND EXPERIMENTAL BOTANY

en

Drought stress reduces plant growth and crop yields. Plants activate defense mechanisms, such as stomatal closure, phenolic compounds biosynthesis, compatible solutes, protein, amino acids, and abscisic acid to cope with drought stress. Most studies on drought stress effects have been focused only on fully expanded leaves, while information on young leaves is scarce. In this study, we investigated the differential photosynthetic performance as well as the primary and secondary metabolic responses in young and fully expanded leaves of Aristotelia chilensis plants subjected to drought stress. We found that drought stress negatively affected net CO2 assimilation (A(N)), stomatal conductance (g(s)), and transpiration (E) more drastically in young leaves than fully expanded leaves of A. chilensis plants. On the other hand, young leaves accumulated higher total protein than fully expanded leaves of plants at day 10 of drought stress. Likewise, the total amino acid was increased in young leaves of stressed plants on days 5, 10, and 20, showing higher values at the end of the experiment. Starch was reduced in both young and fully expanded leaves of drought-stressed plants, while sucrose and fructose levels increased around 2-fold in young leaves at days 10 and 20 of drought stress. We observed that leucine and valine increased in young leaves at days 10 and 20 of drought stress, while in contrast, no changes were observed in the fully expanded leaves. PCA analysis clearly separated the young from fully expanded leaves at 10 and 20 days under drought stress, where anthocyanins and related genes (UFGT and NCED) were stimulated in fully expanded leaves, while in young leaves was amino acids, sucrose, and fructose. Our results demonstrate that young leaves stimulate branched-chain amino acid and phenylalanine accumulation, while fully expanded leaves stimulate anthocyanins and related genes, showing different physiological, biochemical, and molecular mechanisms to respond to drought stress.

artículo original

We would like to thank the Agencia Nacional de Investigacion y Desarrollo (ANID, ex CONICYT) for the PhD. grant to JG-V (CONICYT at N degrees 21130602), FONDECYT POSTDOCTORAL N<SUP>o</SUP>. 3200594, FONDECYT N<SUP>o</SUP> 1171286, and BestPlant Co., Curico, Chile, for providing the maqui plants. The author JG-V thanks Dr. Victor Polanco from Universidad Mayor for kindly providing AcUFGT primer sequences, and Mariela Mora Garrido, biologist, for its collaboration in HPLC-DAD analysis. Research fellowships granted by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil) to RPO-G and AN-N are also gratefully acknowledged.

Agradecemos a la Agencia Nacional de Investigación y Desarrollo (ANID, ex CONICYT) por el doctorado. beca a JG-V (CONICYT en N grados 21130602), FONDECYT POSTDOCTORAL N<SUP>o</SUP>. 3200594, FONDECYT N<SUP>o</SUP> 1171286, y BestPlant Co., Curico, Chile, por proporcionar las plantas de maqui. El autor JG-V agradece al Dr. Víctor Polanco de la Universidad Mayor por proporcionarnos amablemente las secuencias de cebadores AcUFGT, y a Mariela Mora Garrido, bióloga, por su colaboración en el análisis HPLC-DAD. También se agradecen las becas de investigación otorgadas por el Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brasil) a RPO-G y AN-N.

10.1016/j.envexpbot.2022.104814

PDF

Fully-expanded leaves

Water stress

Maqui

Phytohormone

UFGT expression

CHAIN AMINO-ACIDS

ABSCISIC-ACID

WATER-DEFICIT

GENE-EXPRESSION

9-CIS-EPOXYCAROTENOID DIOXYGENASE

CHLOROPHYLL FLUORESCENCE

FLAVONOID BIOSYNTHESIS

PROTEIN-DEGRADATION

STARCH DEGRADATION

GOSSYPIUM-HIRSUTUM

Ciencias Vegetales

Ciencias Ambientales

Hojas completamente expandidas

Estrés hídrico

Maqui

Fitohormona

Expresión UFGT

CADENA DE AMINOÁCIDOS

ÁCIDO-ABSCISICO

DÉFICIT DE AGUA

EXPRESIÓN-GENÉTICA

9-CIS-EPOXICAROTENOIDE DIOXIGENASA

FLUORESCENCIA DE CLOROFILA

BIOSÍNTESIS DE FLAVONOIDES

DEGRADACIÓN DE PROTEÍNAS

ALmidón DEGRADACIÓN

GOSSYPIUM-HIRSUTUM

Differential physiological and metabolic responses in young and fully expanded leaves of Aristotelia chilensis plants subjected to drought stress

artículo original

version publicada

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metadata

metadata

ANID 21130602

ANID-FONDECYT 1171286

ANID-FONDECYT 3200594

ANID FONDECYT 1171286

ANID FONDECYT 3200594

WOS:000760340500003