Comparison between placental and skeletal muscle ECM: in vivo implantation

Primer Autor
Lobo, Sonja E.
Co-autores
Carvalho, Carla Maria F.
Leonel, Luciano C. P. C.
Canada, Rafael R.
Barreto, Rodrigo S. N.
Maria, Durvanei A.
Del Sol, Mariano
Miglino, Maria Angelica
Título
Comparison between placental and skeletal muscle ECM: in vivo implantation
Editorial
TAYLOR & FRANCIS INC
Revista
CONNECTIVE TISSUE RESEARCH
Lenguaje
en
Resumen
Purpose of the study Several tissues have been decellularized and their extracellular matrices used as allogeneic or xenogeneic scaffolds, either in orthotopic or heterotopic implantations, for tissue engineering purposes. Placentas have abundant matrix, extensive microvascular structure, immunomodulatory properties, growth factors and are discarded after birth, representing an interesting source of extracellular matrix. This study aimed at comparing decellularized canine placentas and murine skeletal muscles to regenerate skeletal muscles in a rat model. Materials and Methods Muscle pockets were created at the posterior limbs of male Wistar rats, where the muscle- and placenta-derived extracellular matrices were implanted. Macroscopic, histological, and immunohistochemical analyses were performed after 3, 15, and 45 days of surgeries. Results On the third day, intense inflammatory reaction, with macrophages (CD163(+)) and proliferative cells (PCNA(+)) being observed in control group and adjacent to the decellularized matrices. The percentage of proliferative cells was higher in placenta than in muscle matrices. Macrophages CD163(+ high) were higher in muscles than in placentas, whereas CD163(+ low) were higher in placentas than in muscle ECM, at days 3 and 15. Placental matrices were not completely degraded at day 15, as opposed to the muscular ones. After 45 days, both matrices were resorbed and morphologically normal myofibers, with reduction of cell infiltration, were observed. Conclusions These results demonstrated that xenogeneic placental ECM, implanted heterotopically (representing a biologically critical and challenging microenvironment), induced local inflammatory reactions similar to the allogeneic muscle ECM, implanted orthotopically. Thus, placenta-derived extracellular matrix must be further explored in regenerative medicine.
Tipo de Recurso
artículo original
doi
10.1080/03008207.2020.1834540
Formato Recurso
PDF
Palabras Claves
Decellularization
placenta
skeletal muscle
extracellular matrix
regeneration
EXTRACELLULAR-MATRIX
BIOLOGIC SCAFFOLDS
STEM-CELLS
BIOCOMPATIBILITY
HYDROGEL
EXPRESSION
MODEL
Ubicación del archivo
http://dx.doi.org/10.1080/03008207.2020.1834540
Categoría OCDE
Biología Celular
Ortopedía
Materias
Descelularización
placenta
músculo esquelético
matriz extracelular
regeneración
MATRIZ-EXTRACELULAR
ANDAMIOS BIOLÓGICOS
CÉLULAS MADRE
BIOCOMPATIBILIDAD
HIDROGEL
EXPRESIÓN
MODELO
Disciplinas de la OCDE
Biomateriales (Relacionados con implantes médicos, dispositivos y Sensores)
Biotecnología Relacionada con la Salud
Otras Especialidades de la Biología
Título de la cita (Recomendado-único)
Comparison between placental and skeletal muscle ECM: in vivo implantation
Página de inicio (Recomendado-único)
629
Página final (Recomendado-único)
642
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
Condición de la licencia (Recomendado-repetible)
0
Derechos de acceso
metadata
Access Rights
metadata
Id de Web of Science
WOS:000583912800001
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