Repositorio UFRO · Omeka S

High Presence of NETotic Cells and Neutrophil Extracellular Traps in Vaginal Discharges of Women with Vaginitis: An Exploratory Study

Primer Autor	Sanchez, Raul
Co-autores	Zambrano, Fabiola Melo, Angelica Rivera-Concha, Rodrigo Schulz, Mabel Uribe, Pamela Fonseca-Salamanca, Flery Ossa, Ximena Taubert, Anja Hermosilla, Carlos
Título	High Presence of NETotic Cells and Neutrophil Extracellular Traps in Vaginal Discharges of Women with Vaginitis: An Exploratory Study
Editorial	MDPI
Revista	CELLS
Lenguaje	en
Resumen	Infectious vaginitis is a microbiological syndrome of great importance in public health that affects millions of women worldwide. However, no studies have explored the phenomenon of the production of the neutrophil extracellular traps (NETs) that are released into the female reproductive tract in these pathologies. This study aimed to determine the presence of NETosis in vaginal discharges of women with bacterial vaginosis, candidiasis, and trichomoniasis by characterizing NETs. Extracellular DNA with neutrophil elastase and citrullinated histones was identified to confirm the NET components (n = 10). The concentration, phenotypes of NETs, and number of NETotic cells were determined. The results showed an increase in NETotic cells in women with Candida albicans (CA) and Trichomonas vaginalis (TV) and an increase in NETs in TV-induced vaginitis. Samples of CA- and TV-infected women showed different NET phenotypes (diffNETs, sprNETs, and aggNETs), diffNETs were found in high concentrations in samples with CA and were increased in three types of NETs in TV infections. Samples with intermediate microbiota and bacterial vaginosis showed increased NETotic cells while the intermediate microbiota presented a higher concentration of NETs. Therefore, alterations in the microbiota and the presence of fungal and parasitic infections are important stimuli for the activation and induction of NETosis, and their cytotoxic effects could enhance tissue damage.
Tipo de Recurso	artículo original
Description	This work was supported by the Fondo Nacional de Investigacion Cientifica y Tecnologica (ANID/FONDECYT), Chile (grant number 11200955 to F.Z.) and DIUFRO Nffi DI21-0053, Direccion de Investigacion, Vicerrectoria de Investigacion y Postgrado, Universidad de La Frontera, Temuco, Chile.
doi	10.3390/cells11203185
Formato Recurso	PDF
Palabras Claves	neutrophil extracellular traps Candida albicans Trichomonas vaginalis bacterial vaginosis infectious vaginitis TRICHOMONAS-VAGINALIS BACTERIAL VAGINOSIS CANDIDA-ALBICANS INFECTION EPIDEMIOLOGY INFERTILITY IMMUNITY IMPACT RISK
Ubicación del archivo	http://dx.doi.org/10.3390/cells11203185
Categoría OCDE	Biología Celular
Materias	trampas extracelulares de neutrófilos Candida albicans Trichomonas vaginalis vaginosis bacteriana vaginitis infecciosa TRICHOMONAS-VAGINALIS VAGINOSIS BACTERIANA CANDIDA-ALBICANS INFECCIÓN EPIDEMIOLOGÍA INFERTILIDAD INMUNIDAD IMPACTO RIESGO
Disciplinas de la OCDE	Microbiología Parasitología Micología
Título de la cita (Recomendado-único)	High Presence of NETotic Cells and Neutrophil Extracellular Traps in Vaginal Discharges of Women with Vaginitis: An Exploratory Study
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	version publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID-FONDECYT 11200955 UFRO DIUFRO Nffi DI21-0053 ANID FONDECYT 11200955
Id de Web of Science	WOS:000872453900001