High Presence of NETotic Cells and Neutrophil Extracellular Traps in Vaginal Discharges of Women with Vaginitis: An Exploratory Study
| Primer Autor |
Sanchez, Raul
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| Co-autores |
Zambrano, Fabiola
Melo, Angelica
Rivera-Concha, Rodrigo
Schulz, Mabel
Uribe, Pamela
Fonseca-Salamanca, Flery
Ossa, Ximena
Taubert, Anja
Hermosilla, Carlos
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| Título |
High Presence of NETotic Cells and Neutrophil Extracellular Traps in Vaginal Discharges of Women with Vaginitis: An Exploratory Study
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| Editorial |
MDPI
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| Revista |
CELLS
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| Lenguaje |
en
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| Resumen |
Infectious vaginitis is a microbiological syndrome of great importance in public health that affects millions of women worldwide. However, no studies have explored the phenomenon of the production of the neutrophil extracellular traps (NETs) that are released into the female reproductive tract in these pathologies. This study aimed to determine the presence of NETosis in vaginal discharges of women with bacterial vaginosis, candidiasis, and trichomoniasis by characterizing NETs. Extracellular DNA with neutrophil elastase and citrullinated histones was identified to confirm the NET components (n = 10). The concentration, phenotypes of NETs, and number of NETotic cells were determined. The results showed an increase in NETotic cells in women with Candida albicans (CA) and Trichomonas vaginalis (TV) and an increase in NETs in TV-induced vaginitis. Samples of CA- and TV-infected women showed different NET phenotypes (diffNETs, sprNETs, and aggNETs), diffNETs were found in high concentrations in samples with CA and were increased in three types of NETs in TV infections. Samples with intermediate microbiota and bacterial vaginosis showed increased NETotic cells while the intermediate microbiota presented a higher concentration of NETs. Therefore, alterations in the microbiota and the presence of fungal and parasitic infections are important stimuli for the activation and induction of NETosis, and their cytotoxic effects could enhance tissue damage.
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| Tipo de Recurso |
artículo original
|
| Description |
This work was supported by the Fondo Nacional de Investigacion Cientifica y Tecnologica (ANID/FONDECYT), Chile (grant number 11200955 to F.Z.) and DIUFRO Nffi DI21-0053, Direccion de Investigacion, Vicerrectoria de Investigacion y Postgrado, Universidad de La Frontera, Temuco, Chile.
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| doi |
10.3390/cells11203185
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| Formato Recurso |
PDF
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| Palabras Claves |
neutrophil extracellular traps
Candida albicans
Trichomonas vaginalis
bacterial vaginosis
infectious vaginitis
TRICHOMONAS-VAGINALIS
BACTERIAL VAGINOSIS
CANDIDA-ALBICANS
INFECTION
EPIDEMIOLOGY
INFERTILITY
IMMUNITY
IMPACT
RISK
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| Ubicación del archivo | |
| Categoría OCDE |
Biología Celular
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| Materias |
trampas extracelulares de neutrófilos
Candida albicans
Trichomonas vaginalis
vaginosis bacteriana
vaginitis infecciosa
TRICHOMONAS-VAGINALIS
VAGINOSIS BACTERIANA
CANDIDA-ALBICANS
INFECCIÓN
EPIDEMIOLOGÍA
INFERTILIDAD
INMUNIDAD
IMPACTO
RIESGO
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| Título de la cita (Recomendado-único) |
High Presence of NETotic Cells and Neutrophil Extracellular Traps in Vaginal Discharges of Women with Vaginitis: An Exploratory Study
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
version publicada
|
| License |
CC BY 4.0
|
| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
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| Access Rights |
acceso abierto
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID-FONDECYT 11200955
UFRO DIUFRO Nffi DI21-0053
ANID FONDECYT 11200955
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| Id de Web of Science |
WOS:000872453900001
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cienciaabierta.ufro.cl
ciencia.abierta@ufrontera.cl