Repositorio UFRO · Omeka S

Potent In Vitro Phosphodiesterase 1 Inhibition of Flavone Isolated from Pistacia integerrima Galls

Primer Autor	Rauf, Abdur
Co-autores	Bawazeer, Sami Herrera-Bravo, Jesus Raza, Muslim Naz, Humaira Gul, Somia Muhammad, Naveed Almarhoon, Zainab M. Mabkhot, Yahia N. Ramadan, Mohamed Fawzy Setzer, William N. Dastan, Sevgi Durna Mahmud, Shafi Sharifi-Rad, Javad
Título	Potent In Vitro Phosphodiesterase 1 Inhibition of Flavone Isolated from Pistacia integerrima Galls
Editorial	HINDAWI LTD
Revista	BIOMED RESEARCH INTERNATIONAL
Lenguaje	en
Resumen	To prospect an isozyme-specific, effective inhibitor against the physiologically-crucial enzyme phosphodiesterase 1 (PDE1), phytochemicals from Pistacia integerrima galls were screened. The chloroform fraction of gall extract was subjected to column chromatographic which led to the isolation of compound 1, elucidated to be 5-hydroxy-7-methoxy-2-(4-methoxyphenyl)-4H-chromen-4-one (a flavone). In vitro and in silico PDE1 inhibitory activity of the compound 1 was investigated. EDTA, a known PDE1 inhibitor, was used as the reference. The flavone exhibited in vitro attenuation towards snake venom PDE1. IC50 response was superior to the standard chelator. An in silico molecular docking study was carried out using 3D structure of PDE1 to study the binding interactions of compound 1. The docking study predicted that flavone had a lower binding affinity (-7.6 kcal/mol) and total energy (-95 kcal/mol) score compared to EDTA. The minimal energy associated with the ligand-protein complex implied that isolated compound 1 can serve as a therapeutic agent against PDE1 enzyme-provoked ailments like asthma, hypertension, schizophrenia, and erectile dysfunction.
Tipo de Recurso	artículo original
doi	10.1155/2022/6116003
Formato Recurso	PDF
Palabras Claves	PISTAGREMIC ACID ANTIOXIDANT EXTRACT DOCKING UPDATE LEAVES VENOM
Ubicación del archivo	http://dx.doi.org/10.1155/2022/6116003
Categoría OCDE	Biotecnología y Microbiología Aplicada Medicina Investigación y Experimental
Materias	ÁCIDO PISTAGRÁMICO ANTIOXIDANTE EXTRACTO DOCKING ACTUALIZACIÓN HOJAS VENENO
Disciplinas de la OCDE	Bioquímica Farmacología y Farmacia Otros Temas de Medicina Básica
Título de la cita (Recomendado-único)	Potent In Vitro Phosphodiesterase 1 Inhibition of Flavone Isolated from Pistacia integerrima Galls
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	version publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:000810504800004