Anthocyanins Found in Pinot Noir Waste Induce Target Genes Related to the Nrf2 Signalling in Endothelial Cells
| Primer Autor |
Salazar, Luis A.
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| Co-autores |
Herrera-Bravo, Jesus
Beltran, Jorge F.
Huard, Nolberto
Saavedra, Kathleen
Saavedra, Nicolas
Alvear, Marysol
Lanas, Fernando
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| Título |
Anthocyanins Found in Pinot Noir Waste Induce Target Genes Related to the Nrf2 Signalling in Endothelial Cells
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| Editorial |
MDPI
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| Revista |
ANTIOXIDANTS
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| Lenguaje |
en
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| Resumen |
Grape pomace is a source of anthocyanins, which can prevent cardiovascular diseases due to their antioxidant properties. Anthocyanin activity is associated with the ability to regulate oxidative stress through the transcription factor Nrf2. Thus, the present study aimed to evaluate if the anthocyanins found in Pinot noir pomace extract can affect the target genes related to the Nrf2 signalling pathway in endothelial cells. Our results highlight that the predominant anthocyanin in the Pinot noir pomace extract was malvidin-3-glucoside (3.7 +/- 2.7 Eq. Malv-3-glu/kg). Molecular docking indicated that cyanidin-3-glucoside (-6.9 kcal/mol), malvidin-3-glucoside (-6.6 kcal/mol) and peonidin-3-glucoside (-6.6 kcal/mol) showed the highest affinities for the binding sites of the BTB domains in Keap1, suggesting that these components may modify the interaction of this protein with Nrf2. In addition, when HUVEC cells were exposed to different concentrations of Pinot noir pomace extract (100 mu g/mL, 200 mu g/mL, and 400 mu g/mL), no changes in Nrf2 gene expression were observed. However, the gene expression of HO-1 and NQO1, which are in the signalling pathway of this transcription factor, increased according the concentrations of the extract (p = 0.0004 and p = 0.0084, respectively). In summary, our results show that anthocyanins play a very important role in Nrf2 activation and release, while at the same time not promoting its transcription. These preliminary results strongly suggest that the Pinot noir pomace extract can serve as a potent bioactive component source that protects cells against oxidative stress.
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| Tipo de Recurso |
artículo original
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| Description |
This research was funded by FONDECYT-Chile (Grant numbers 1171765 and 1171618) and Direccion de Investigacion of the Universidad de La Frontera (Grant number DI19-2018).
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| doi |
10.3390/antiox11071239
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| Formato Recurso |
PDF
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| Palabras Claves |
grape pomace
oxidative stress
bioactive compounds
protective effect
agro-industrial waste
wine industry
anthocyanins
HO-1
NQO1
ANTIOXIDANT ACTIVITY
KEAP1-NRF2 SYSTEM
OXIDATIVE STRESS
HEME OXYGENASE-1
CARBON-MONOXIDE
EXPRESSION
LEAVES
DPPH
OPTIMIZATION
POLYPHENOL
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| Ubicación del archivo | |
| Categoría OCDE |
Bioquímica y Biología Molecular
Química Medicinal
Ciencia y tecnología de los alimentos
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| Materias |
orujo de uva
estrés oxidativo
compuestos bioactivos
efecto protector
residuos agroindustriales
industria vitivinícola
antocianinas
HO-1
NQO1
ACTIVIDAD ANTIOXIDANTE
SISTEMA KEAP1-NRF2
ESTRÉS OXIDATIVO
HEME OXIGENASA-1
MONÓXIDO DE CARBONO
EXPRESIÓN
HOJAS
DPPH
OPTIMIZACIÓN
POLIFENOL
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| Título de la cita (Recomendado-único) |
Anthocyanins Found in Pinot Noir Waste Induce Target Genes Related to the Nrf2 Signalling in Endothelial Cells
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| Identificador del recurso (Mandatado-único) |
artículo original
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| Versión del recurso (Recomendado-único) |
version publicada
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| License |
CC BY 4.0
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| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
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| Derechos de acceso |
acceso abierto
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| Access Rights |
acceso abierto
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID-FONDECYT 1171765
ANID-FONDECYT 1171618
ANID FONDECYT 1171765
ANID FONDECYT 1171618
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| Id de Web of Science |
WOS:000831882200001
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cienciaabierta.ufro.cl
ciencia.abierta@ufrontera.cl