Repositorio
Institucional

Salazar, Luis A.

Herrera-Bravo, Jesus

Beltran, Jorge F.

Huard, Nolberto

Saavedra, Kathleen

Saavedra, Nicolas

Alvear, Marysol

Lanas, Fernando

Anthocyanins Found in Pinot Noir Waste Induce Target Genes Related to the Nrf2 Signalling in Endothelial Cells

MDPI

ANTIOXIDANTS

en

Grape pomace is a source of anthocyanins, which can prevent cardiovascular diseases due to their antioxidant properties. Anthocyanin activity is associated with the ability to regulate oxidative stress through the transcription factor Nrf2. Thus, the present study aimed to evaluate if the anthocyanins found in Pinot noir pomace extract can affect the target genes related to the Nrf2 signalling pathway in endothelial cells. Our results highlight that the predominant anthocyanin in the Pinot noir pomace extract was malvidin-3-glucoside (3.7 +/- 2.7 Eq. Malv-3-glu/kg). Molecular docking indicated that cyanidin-3-glucoside (-6.9 kcal/mol), malvidin-3-glucoside (-6.6 kcal/mol) and peonidin-3-glucoside (-6.6 kcal/mol) showed the highest affinities for the binding sites of the BTB domains in Keap1, suggesting that these components may modify the interaction of this protein with Nrf2. In addition, when HUVEC cells were exposed to different concentrations of Pinot noir pomace extract (100 mu g/mL, 200 mu g/mL, and 400 mu g/mL), no changes in Nrf2 gene expression were observed. However, the gene expression of HO-1 and NQO1, which are in the signalling pathway of this transcription factor, increased according the concentrations of the extract (p = 0.0004 and p = 0.0084, respectively). In summary, our results show that anthocyanins play a very important role in Nrf2 activation and release, while at the same time not promoting its transcription. These preliminary results strongly suggest that the Pinot noir pomace extract can serve as a potent bioactive component source that protects cells against oxidative stress.

artículo original

This research was funded by FONDECYT-Chile (Grant numbers 1171765 and 1171618) and Direccion de Investigacion of the Universidad de La Frontera (Grant number DI19-2018).

10.3390/antiox11071239

PDF

grape pomace

oxidative stress

bioactive compounds

protective effect

agro-industrial waste

wine industry

anthocyanins

HO-1

NQO1

ANTIOXIDANT ACTIVITY

KEAP1-NRF2 SYSTEM

OXIDATIVE STRESS

HEME OXYGENASE-1

CARBON-MONOXIDE

EXPRESSION

LEAVES

DPPH

OPTIMIZATION

POLYPHENOL

Bioquímica y Biología Molecular

Química Medicinal

Ciencia y tecnología de los alimentos

orujo de uva

estrés oxidativo

compuestos bioactivos

efecto protector

residuos agroindustriales

industria vitivinícola

antocianinas

HO-1

NQO1

ACTIVIDAD ANTIOXIDANTE

SISTEMA KEAP1-NRF2

ESTRÉS OXIDATIVO

HEME OXIGENASA-1

MONÓXIDO DE CARBONO

EXPRESIÓN

HOJAS

DPPH

OPTIMIZACIÓN

POLIFENOL

Viticultura

Bioquímica

Biología Molecular

Anthocyanins Found in Pinot Noir Waste Induce Target Genes Related to the Nrf2 Signalling in Endothelial Cells

artículo original

version publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

ANID-FONDECYT 1171765

ANID-FONDECYT 1171618

ANID FONDECYT 1171765

ANID FONDECYT 1171618

WOS:000831882200001