Repositorio UFRO · Omeka S

beta-Carotene Increases Activity of Cytochrome P450 2E1 during Ethanol Consumption

Primer Autor	Sandoval, Cristian
Co-autores	Mella, Luciana Godoy, Karina Adeli, Khosrow Farias, Jorge
Título	beta-Carotene Increases Activity of Cytochrome P450 2E1 during Ethanol Consumption
Editorial	MDPI
Revista	ANTIOXIDANTS
Lenguaje	en
Resumen	One of the key routes through which ethanol induces oxidative stress appears to be the activation of cytochrome P450 2E1 at different levels of ethanol intake. Our aim was to determine if oral beta-carotene intake had an antioxidant effect on CYP2E1 gene expression in mice that had previously consumed ethanol. C57BL/6 mice were used and distributed into: control (C), low-dose alcohol (LA), moderate-dose alcohol (MA), beta-carotene (B), low-dose alcohol+beta-carotene (LA + B), and moderate-dose alcohol+beta-carotene (MA + B). Animals were euthanized at the end of the experiment, and liver tissue was taken from each one. CYP2E1 was measured using qPCR to detect liver damage. The relative expression level of each RNA was estimated using the comparative threshold cycle (Ct) technique (2(-Delta Delta CT) method) by averaging the Ct values from three replicates. The LA+B (2267 +/- 0.707) and MA+B (2.307 +/- 0.384) groups had the highest CYP2E1 fold change values. On the other hand, the C (1.053 +/- 0.292) and LA (1.240 +/- 0.163) groups had the lowest levels. These results suggest that ethanol feeding produced a fold increase in CYP2E1 protein in mice as compared to the control group. Increased CYP2E1 activity was found to support the hypothesis that beta-carotene might be dangerous during ethanol exposure in animal models. Our findings imply that beta-carotene can increase the hepatic damage caused by low and high doses of alcohol. Therefore, the quantity of alcohol ingested, the exposure period, the regulatory mechanisms of alcoholic liver damage, and the signaling pathways involved in the consumption of both alcohol and antioxidant must all be considered.
Tipo de Recurso	artículo original
doi	10.3390/antiox11051033
Formato Recurso	PDF
Palabras Claves	alcohol intake alcoholic fatty liver disease antioxidant treatment chronic alcohol consumption ALCOHOL-CONSUMPTION OXIDATIVE STRESS QUANTITATIVE PCR OXIDIZING SYSTEM LIVER-DISEASE INDUCTION CYP2E1 RISK SUPPLEMENTATION MITOCHONDRIA
Ubicación del archivo	http://dx.doi.org/10.3390/antiox11051033
Categoría OCDE	Bioquímica y Biología Molecular Química Medicinal Ciencia y tecnología de los alimentos
Materias	ingesta de alcohol enfermedad del hígado graso alcohólico tratamiento antioxidante consumo crónico de alcohol CONSUMO DE ALCOHOL ESTRÉS OXIDATIVO PCR CUANTITATIVA SISTEMA OXIDANTE ENFERMEDAD DEL HÍGADO INDUCCIÓN CYP2E1 RIESGO SUPLEMENTACIÓN MITOCONDRIA
Disciplinas de la OCDE	Toxicología Bioquímica Otras Especialidades de la Biología
Versión del recurso (Recomendado-único)	version publicada
Tipo de ruta	dorada verde
Access Rights	acceso abierto
Derechos de acceso	acceso abierto
Id de Web of Science	WOS:000801427700001
Título de la cita (Recomendado-único)	beta-Carotene Increases Activity of Cytochrome P450 2E1 during Ethanol Consumption
Identificador del recurso (Mandatado-único)	artículo original
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0