beta-Carotene Increases Activity of Cytochrome P450 2E1 during Ethanol Consumption

Primer Autor
Sandoval, Cristian
Co-autores
Mella, Luciana
Godoy, Karina
Adeli, Khosrow
Farias, Jorge
Título
beta-Carotene Increases Activity of Cytochrome P450 2E1 during Ethanol Consumption
Editorial
MDPI
Revista
ANTIOXIDANTS
Lenguaje
en
Resumen
One of the key routes through which ethanol induces oxidative stress appears to be the activation of cytochrome P450 2E1 at different levels of ethanol intake. Our aim was to determine if oral beta-carotene intake had an antioxidant effect on CYP2E1 gene expression in mice that had previously consumed ethanol. C57BL/6 mice were used and distributed into: control (C), low-dose alcohol (LA), moderate-dose alcohol (MA), beta-carotene (B), low-dose alcohol+beta-carotene (LA + B), and moderate-dose alcohol+beta-carotene (MA + B). Animals were euthanized at the end of the experiment, and liver tissue was taken from each one. CYP2E1 was measured using qPCR to detect liver damage. The relative expression level of each RNA was estimated using the comparative threshold cycle (Ct) technique (2(-Delta Delta CT) method) by averaging the Ct values from three replicates. The LA+B (2267 +/- 0.707) and MA+B (2.307 +/- 0.384) groups had the highest CYP2E1 fold change values. On the other hand, the C (1.053 +/- 0.292) and LA (1.240 +/- 0.163) groups had the lowest levels. These results suggest that ethanol feeding produced a fold increase in CYP2E1 protein in mice as compared to the control group. Increased CYP2E1 activity was found to support the hypothesis that beta-carotene might be dangerous during ethanol exposure in animal models. Our findings imply that beta-carotene can increase the hepatic damage caused by low and high doses of alcohol. Therefore, the quantity of alcohol ingested, the exposure period, the regulatory mechanisms of alcoholic liver damage, and the signaling pathways involved in the consumption of both alcohol and antioxidant must all be considered.
Tipo de Recurso
artículo original
doi
10.3390/antiox11051033
Formato Recurso
PDF
Palabras Claves
alcohol intake
alcoholic fatty liver disease
antioxidant treatment
chronic alcohol consumption
ALCOHOL-CONSUMPTION
OXIDATIVE STRESS
QUANTITATIVE PCR
OXIDIZING SYSTEM
LIVER-DISEASE
INDUCTION
CYP2E1
RISK
SUPPLEMENTATION
MITOCHONDRIA
Ubicación del archivo
http://dx.doi.org/10.3390/antiox11051033
Categoría OCDE
Bioquímica y Biología Molecular
Química Medicinal
Ciencia y tecnología de los alimentos
Materias
ingesta de alcohol
enfermedad del hígado graso alcohólico
tratamiento antioxidante
consumo crónico de alcohol
CONSUMO DE ALCOHOL
ESTRÉS OXIDATIVO
PCR CUANTITATIVA
SISTEMA OXIDANTE
ENFERMEDAD DEL HÍGADO
INDUCCIÓN
CYP2E1
RIESGO
SUPLEMENTACIÓN
MITOCONDRIA
Disciplinas de la OCDE
Toxicología
Bioquímica
Otras Especialidades de la Biología
Título de la cita (Recomendado-único)
beta-Carotene Increases Activity of Cytochrome P450 2E1 during Ethanol Consumption
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:000801427700001
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Colección Publicaciones Científicas
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