Repositorio
Institucional

Hermosilla, Edward

Diaz, Marcela

Vera, Joelis

Seabra, Amedea B.

Tortella, Gonzalo

Parada, Javiera

Rubilar, Olga

Molecular Weight Identification of Compounds Involved in the Fungal Synthesis of AgNPs: Effect on Antimicrobial and Photocatalytic Activity

MDPI

ANTIBIOTICS-BASEL

en

The biological synthesis of silver nanoparticles (AgNPs) for medical, environmental, and industrial applications is considered an alternative to chemical synthesis methods. Additionally, the reducing, capping, and stabilizing molecules produced by the organisms can play a key role in the further activity of AgNPs. In this work, we evaluated the synthesis of AgNPs by four molecular weight fractions (S1: <10 kDa, S2: 10 to 30 kDa, S3: 30 to 50 kDa, and S4: >50 kDa) of mycelia-free aqueous extract produced by the white-rot fungus Stereum hirsutum and their effect on the antimicrobial activity against Pseudomonas syringae and photocatalytic decolorization of nine synthetic dyes exposed to sunlight radiation. All synthesis assay fractions showed the characteristic surface plasmon resonance (SPR) with 403 to 421 nm peaks. TEM analysis of synthesized AgNPs showed different sizes: the whole mycelia-free extracts S0 (13.8 nm), S1 (9.06 nm), S2 (10.47 nm), S3 (22.48 nm), and S4 (16.92 nm) fractions. The results of disk diffusion assays showed an inverse relation between antimicrobial activity and the molecular weight of compounds present in the mycelia-free aqueous extract used to synthesize AgNPs. The AgNPs synthesized by S0 (14.3 mm) and S1(14.2 mm) generated the highest inhibition diameter of P. syringae growth. By contrast, in the photocatalytic assays, the AgNPs synthesized by the S2 fraction showed the highest discoloration in all the dyes tested, reaching 100% of the discoloration of basic dyes after 2 h of sunlight exposure. The maximum discoloration observed in reactive and acid dyes was 53.2% and 65.3%, respectively. This differentiation in the antimicrobial and photocatalytic activity of AgNPs could be attributed to the capping effect of the molecules present in the extract fractions. Therefore, the molecular separation of synthesis extract enables the specific activities of the AgNPs to be enhanced.

artículo original

This research was funded by ANID/FONDECYT Regular 1191089, ANID/FONDECYT Post-doctoral project 3190922, Processo Fapesp 2018/08194-2, and ANID/FONDAP/15130015. The APC was funded by ANID/FONDECYT Regular 1191089.

Esta investigación fue financiada por ANID/FONDECYT Regular 1191089, Proyecto Postdoctoral ANID/FONDECYT 3190922, Proceso Fapesp 2018/08194-2 y ANID/FONDAP/15130015. El APC fue financiado por ANID/FONDECYT Regular 1191089.

10.3390/antibiotics11050622

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antimicrobial

photocatalysis

fungal synthesis

synthesis mechanism

silver nanoparticles

SILVER NANOPARTICLES

GREEN SYNTHESIS

LEAF EXTRACT

Enfermedades infecciosas

Farmacología y farmacia

antimicrobiano

fotocatálisis

síntesis de hongos

mecanismo de síntesis

nanopartículas de plata

NANOPARTÍCULAS DE PLATA

SÍNTESIS VERDE

EXTRACTO DE HOJA

Biotecnología Ambiental

Microbiología

Físico-Química

Molecular Weight Identification of Compounds Involved in the Fungal Synthesis of AgNPs: Effect on Antimicrobial and Photocatalytic Activity

artículo original

version publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

ANID-FONDECYT 1191089

ANID-FONDECYT 3190922

FAPESP 2018/08194-2

ANID-FONDAP 15130015

ANID FONDECYT 1191089

ANID FONDECYT 3190922

ANID FONDAP 15130015

WOS:000802563200001