Repositorio
Institucional

Felmer, Ricardo

Arias, Maria Elena

Vargas, Tamara

Gallardo, Victor

Aguila, Luis

Simple and Efficient Chemically Defined In Vitro Maturation and Embryo Culture System for Bovine Embryos

MDPI

ANIMALS

en

Supplementation of the culture media for in vitro production (IVP) of bovine embryos with fetal bovine serum (FBS) is associated with inconsistent outcomes. The present study sought to replace FBS and BSA by insulin-like growth factor 1 (IGF1), fibroblast growth factor 2 (FGF2) and epidermal growth factor (EGF). In Experiment 1, absence of FBS from maturation medium (MM) did not affect the rate of in vitro maturation, as assessed by the extrusion of the first polar body. However, when gonadotropins and FBS were removed from the MM, the maturation rate was significantly reduced even in the presence of growth factors. Therefore, gonadotropin-supplemented MM medium was established as the base medium for the defined maturation condition. In Experiment 2, the addition of growth factors to gonadotropin-supplemented MM medium supported similar maturation (similar to 90%) compared to the undefined condition (FBS-carrying). In Experiment 3, the addition of growth factors to embryo culture medium showed similar in vitro competence compared to the undefined (FBS) control. In Experiment 4, completely defined conditions (absence of FBS and BSA during in vitro maturation and embryo culture) were tested. A higher cleavage was observed with FGF2 (86%) compared to EGF (77%) and the FBS control (77%), but similar blastocyst rates were observed for FGF2 (24%), EGF (19%) and the FBS control (25%). Embryo quality was similar among groups. Finally, post-thawing survival was higher for FGF2 (94%) compared to the FBS control (77%). Thus, we report a simple defined WP system for bovine species that generates developmental outcomes and embryos of similar quality than those produced under conditions containing FBS.

artículo original

This research was funded by ANID-Chile, grant FONDECYT 1201166 and Universidad de La Frontera, Programa de Formacion de Investigadores Postdoctorales (PDT21-0001).

10.3390/ani12213057

PDF

chemically defined

IVF

zygote

bovine oocyte

EGF

embryos

FGF2 and IGF1

GROWTH-FACTOR-I

PROTEIN-FREE MEDIUM

FETAL CALF SERUM

IGF-I

OOCYTE MATURATION

DEVELOPMENTAL COMPETENCE

BLASTOCYST DEVELOPMENT

PREIMPLANTATION DEVELOPMENT

CELL NUMBER

INSULIN

Agricultura

Lácteos y Ciencia Animal

Ciencias Veterinarias

Zoología

químicamente definido

FIV

cigoto

ovocito bovino

EGF

embriones

FGF2 e IGF1

FACTOR DE CRECIMIENTO I

MEDIO LIBRE DE PROTEÍNAS

SUERO DE TERNERA FETAL

IGF-I

MADURACIÓN DE OOCITOS

COMPETENCIA DE DESARROLLO

DESARROLLO DE BLASTOQUISTOS

DESARROLLO PREIMPLANTACIÓN

CÉLULA NÚMERO

INSULINA

Ciencia Animal y lechería

Biología Reproductiva

Biotecnología agrícola

Simple and Efficient Chemically Defined In Vitro Maturation and Embryo Culture System for Bovine Embryos

artículo original

version publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

ANID-FONDECYT 1201166

UFRO PDT21-0001

ANID FONDECYT 1201166

WOS:000880712200001