Simple and Efficient Chemically Defined In Vitro Maturation and Embryo Culture System for Bovine Embryos
| Primer Autor |
Felmer, Ricardo
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| Co-autores |
Arias, Maria Elena
Vargas, Tamara
Gallardo, Victor
Aguila, Luis
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| Título |
Simple and Efficient Chemically Defined In Vitro Maturation and Embryo Culture System for Bovine Embryos
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| Editorial |
MDPI
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| Revista |
ANIMALS
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| Lenguaje |
en
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| Resumen |
Supplementation of the culture media for in vitro production (IVP) of bovine embryos with fetal bovine serum (FBS) is associated with inconsistent outcomes. The present study sought to replace FBS and BSA by insulin-like growth factor 1 (IGF1), fibroblast growth factor 2 (FGF2) and epidermal growth factor (EGF). In Experiment 1, absence of FBS from maturation medium (MM) did not affect the rate of in vitro maturation, as assessed by the extrusion of the first polar body. However, when gonadotropins and FBS were removed from the MM, the maturation rate was significantly reduced even in the presence of growth factors. Therefore, gonadotropin-supplemented MM medium was established as the base medium for the defined maturation condition. In Experiment 2, the addition of growth factors to gonadotropin-supplemented MM medium supported similar maturation (similar to 90%) compared to the undefined condition (FBS-carrying). In Experiment 3, the addition of growth factors to embryo culture medium showed similar in vitro competence compared to the undefined (FBS) control. In Experiment 4, completely defined conditions (absence of FBS and BSA during in vitro maturation and embryo culture) were tested. A higher cleavage was observed with FGF2 (86%) compared to EGF (77%) and the FBS control (77%), but similar blastocyst rates were observed for FGF2 (24%), EGF (19%) and the FBS control (25%). Embryo quality was similar among groups. Finally, post-thawing survival was higher for FGF2 (94%) compared to the FBS control (77%). Thus, we report a simple defined WP system for bovine species that generates developmental outcomes and embryos of similar quality than those produced under conditions containing FBS.
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| Tipo de Recurso |
artículo original
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| Description |
This research was funded by ANID-Chile, grant FONDECYT 1201166 and Universidad de La Frontera, Programa de Formacion de Investigadores Postdoctorales (PDT21-0001).
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| doi |
10.3390/ani12213057
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| Formato Recurso |
PDF
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| Palabras Claves |
chemically defined
IVF
zygote
bovine oocyte
EGF
embryos
FGF2 and IGF1
GROWTH-FACTOR-I
PROTEIN-FREE MEDIUM
FETAL CALF SERUM
IGF-I
OOCYTE MATURATION
DEVELOPMENTAL COMPETENCE
BLASTOCYST DEVELOPMENT
PREIMPLANTATION DEVELOPMENT
CELL NUMBER
INSULIN
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| Ubicación del archivo | |
| Categoría OCDE |
Agricultura
Lácteos y Ciencia Animal
Ciencias Veterinarias
Zoología
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| Materias |
químicamente definido
FIV
cigoto
ovocito bovino
EGF
embriones
FGF2 e IGF1
FACTOR DE CRECIMIENTO I
MEDIO LIBRE DE PROTEÍNAS
SUERO DE TERNERA FETAL
IGF-I
MADURACIÓN DE OOCITOS
COMPETENCIA DE DESARROLLO
DESARROLLO DE BLASTOQUISTOS
DESARROLLO PREIMPLANTACIÓN
CÉLULA NÚMERO
INSULINA
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| Título de la cita (Recomendado-único) |
Simple and Efficient Chemically Defined In Vitro Maturation and Embryo Culture System for Bovine Embryos
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| Identificador del recurso (Mandatado-único) |
artículo original
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| Versión del recurso (Recomendado-único) |
version publicada
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| License |
CC BY 4.0
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| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
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| Derechos de acceso |
acceso abierto
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| Access Rights |
acceso abierto
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID-FONDECYT 1201166
UFRO PDT21-0001
ANID FONDECYT 1201166
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| Id de Web of Science |
WOS:000880712200001
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cienciaabierta.ufro.cl
ciencia.abierta@ufrontera.cl