Spermatological characteristics and effects of cryopreservation in Lebranche mullet spermatozoa (Mugil liza Valenciennes, 1836): First report of ultra-rapid freezing

Primer Autor
Figueroa, Elías
Co-autores
Magnotti, C.
Cerqueira, V.
Villasante, A.
Romero, J.
Watanabe, I.
Oliveira, R. P. S.
Farias, J.
Merino, O.
Valdebenito
Título
Spermatological characteristics and effects of cryopreservation in Lebranche mullet spermatozoa (Mugil liza Valenciennes, 1836): First report of ultra-rapid freezing
Editorial
ELSEVIER
Revista
ANIMAL REPRODUCTION SCIENCE
Lenguaje
en
Resumen
The present study investigated the spermatological characteristics of raw semen of Lebranche mullet (Mugil liza), namely pH, and sperm density, and motility, and subsequently evaluated the effects of different times of exposure to cryoprotectants, and the application of an ultra-rapid freezing protocol, on sperm motility and plasma membrane integrity. Semen samples were analyzed undiluted (control) and diluted 1:50 v/v in CF-HBSS + 10% Dimethyl sulfoxide + 30% Ethylene glycol + 94.58 gL-1 Trehalose dehydrate (n = 15). Two treatments - diluted semen samples in cryoprotective medium without ultra-rapid freezing (T1), and diluted semen in cryoprotective medium with ultra-rapid freezing (T2) - were evaluated at 0, 2, 4, 6 and 8 min. The frozen samples were thawed at 37oC for 60 s. The spermatological characteristics recorded for the semen were: pH: 7.57 +/- 0.21, sperm density: 30.4 +/- 2.9 x 109 sperm mL-1, motility: 82 +/- 4.9%. Sperm motility presented differences after 2 min exposure to cryoprotectants (70.0 +/- 2.7%) and ultra-rapid freezing (66.5 +/- 5.8%) compared to the control group (98.5 +/- 1.9% and 98.5 +/- 2.1%, respectively, p < 0.05). On the other hand, the plasma membrane integrity of the spermatozoa after 2 min exposure to cryoprotectants (64.0 +/- 8.6%) and ultra-rapid freezing (62.5 +/- 5.2%) presented no differences compared to the control group (69.5 +/- 3.9% and 70.0 +/- 3.5%, respectively p > 0.05), however, differences were observed in the parameters evaluated after longer exposure and cryopreservation times. This is the first report evaluating the effects of different times of exposure to cryoprotectants and direct ultra-rapid freezing in liquid nitrogen on Mugil liza sperm. Our results demonstrated the protocol of sperm ultra-freezing is safe within a time ' s window of 2 min of exposure to cryoprotectants, after which a toxicity effect on sperm can be observed.
Tipo de Recurso
artículo original
Description
The authors are grateful to the team of LAPMAR - UFSC, and for the financial support of CAPES (No 23038.004323/2014-54) and CNPq (No 422460/2016-8, No 430770/2018-9) in Brazil, and FONDECYT/ POSTDOCTORAL (No 3180765 and No 3210593), FONDECYT/REGULAR (No 1211246) and CLIMAT-AmSub (No CLI2020005) projects in Chile. Also, we thank Leonel Herrera Martinez for his support in revising the English.
Los autores agradecen al equipo de LAPMAR - UFSC, y por el apoyo financiero de CAPES (No 23038.004323/2014-54) y CNPq (No 422460/2016-8, No 430770/2018-9) en Brasil, y FONDECYT/ Proyectos POSTDOCTORAL (No 3180765 y No 3210593), FONDECYT/REGULAR (No 1211246) y CLIMAT-AmSub (No CLI2020005) en Chile. Además, agradecemos a Leonel Herrera Martínez por su apoyo en la revisión del inglés.
The authors are grateful to the team of LAPMAR - UFSC, and for the financial support of CAPES (No 23038.004323/2014-54) and CNPq (No 422460/2016-8; No 430770/2018-9) in Brazil, and FONDECYT/ POSTDOCTORAL (No 3180765 and No 3210593), FONDECYT/REGULAR (No 1211246) and CLIMAT-AmSub (No CLI2020005) projects in Chile. Also, we thank Leonel Herrera Martinez for his support in revising the English.
Los autores agradecen al equipo de LAPMAR - UFSC, y por el apoyo financiero de CAPES (No 23038.004323/2014-54) y CNPq (No 422460/2016-8; No 430770/2018-9) en Brasil, y FONDECYT/ Proyectos POSTDOCTORAL (No 3180765 y No 3210593), FONDECYT/REGULAR (No 1211246) y CLIMAT-AmSub (No CLI2020005) en Chile. Además, agradecemos a Leonel Herrera Martínez por su apoyo en la revisión del inglés.
doi
10.1016/j.anireprosci.2022.106986
Formato Recurso
PDF
Palabras Claves
Spermatology
Ultra-rapid freezing
Motility
Sperm plasma membrane
Mugilidae
CRYOPROTECTANT-FREE VITRIFICATION
SPERM CRYOPRESERVATION
REFRIGERATED STORAGE
ETHYLENE-GLYCOL
SEMINAL PLASMA
TOXICITY
MOTILITY
MATURATION
MIGRATION
SURVIVAL
Ubicación del archivo
http://dx.doi.org/10.1016/j.anireprosci.2022.106986
Categoría OCDE
Agricultura
Lácteos y Ciencia Animal
Biología Reproductiva
Ciencias Veterinarias
Materias
Espermatología
Congelación ultrarrápida
Motilidad
Membrana plasmática de esperma
Mugilidae
VITRIFICACIÓN SIN CRIOPROTECTANTE
CRIOCONSERVACIÓN DE ESPERMA
ALMACENAMIENTO REFRIGERADO
ETILENGLICOL
PLASMA SEMINAL
TOXICIDAD
MOTILIDAD
MADURACIÓN
MIGRACIÓN
SUPERVIVENCIA
Disciplinas de la OCDE
Pesca
Biología Marina y Limnología
Biología Reproductiva
Título de la cita (Recomendado-único)
Spermatological characteristics and effects of cryopreservation in Lebranche mullet spermatozoa (Mugil liza Valenciennes, 1836): First report of ultra-rapid freezing
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
version publicada
Condición de la licencia (Recomendado-repetible)
0
Derechos de acceso
metadata
Access Rights
metadata
Referencia del Financiador (Mandatado si es aplicable-repetible)
CAPES 23038.004323/2014-54
CNPQ 422460/2016-8
CNPQ 430770/2018-9
ANID-FONDECYT 3180765
ANID-FONDECYT 3210593
ANID-FONDECYT 1211246
ANID-CLIMAT CLI2020005
ANID FONDECYT 3180765
ANID FONDECYT 3210593
ANID FONDECYT 1211246
ANID CLIMAT CLI2020005
Id de Web of Science
WOS:000806560300004
Colecciones
Colección Publicaciones Científicas
Colección ANID
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