Helicobacter pyloril-asparaginase: a study of immunogenicity from an in silico approach
| Primer Autor |
Farias, Jorge G.
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| Co-autores |
Herrera Belen, Lisandra
Beltran, Jorge F.
Pessoa, Adalberto
Castillo, Rodrigo L.
de Oliveira Rangel-Yagui, Carlota
|
| Título |
Helicobacter pyloril-asparaginase: a study of immunogenicity from an in silico approach
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| Editorial |
SPRINGER HEIDELBERG
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| Revista |
3 BIOTECH
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| Lenguaje |
en
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| Resumen |
Helicobacter pylori has become the causal agent of multiple forms of gastric disease worldwide, including gastric cancer. The enzyme l-asparaginase (ASNase) has been studied as a virulence factor. In this work, we performed an in silico investigation to characterize the immunological profile of H. pylori ASNase (HpASNase) to ascertain the possible implication of HpASNase immunogenicity in the H. pylori virulence mechanism. We applied a workflow based on bioinformatics tools, which, by calculating the relative frequency of immunogenic T-cell and B-cell epitopes, allowed us to predict the immunogenicity and allergenicity of HpASNase in silico. We also visualized the epitopes by mapping them into the native structure of the enzyme. We report for the first time the T-cell and B-cell epitope composition that contributes to the immunogenicity of this HpASNase, as well as the regions that could generate a hypersensitivity response in humans. ASNase from H. pylori resulted in highly immunogenic and allergenic. The high immunogenicity of HpASNase could imply the pathogenic mechanisms of H. pylori. This knowledge could be important for the development of new drugs against H. pylori infections.
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| Tipo de Recurso |
artículo original
|
| Description |
We acknowledge the Agencia Nacional de Investigacion y Desarrollo (ANID) Fellowship No. 21170061 and the National Council for Scientific and Technological Development (CNPq/Brazil, Fellowship 301832/2017-0) for supporting this work.
Agradecemos a la Beca de la Agencia Nacional de Investigación y Desarrollo (ANID) No. 21170061 y al Consejo Nacional de Desarrollo Científico y Tecnológico (CNPq/Brasil, Beca 301832/2017-0) por apoyar este trabajo.
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| doi |
10.1007/s13205-022-03359-0
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| Formato Recurso |
PDF
|
| Palabras Claves |
l-Asparaginase
Helicobacter pylori
Bioinformatics
Immunogenicity
Virulence factor
INFECTION
VACCINES
GENE
|
| Ubicación del archivo | |
| Categoría OCDE |
Biotecnología y Microbiología Aplicada
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| Materias |
l-asparaginasa
Helicobacter pylori
Bioinformática
Inmunogenicidad
Factor de virulencia
INFECCIÓN
VACUNAS
GEN
|
| Título de la cita (Recomendado-único) |
Helicobacter pyloril-asparaginase: a study of immunogenicity from an in silico approach
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
version publicada
|
| Condición de la licencia (Recomendado-repetible) |
0
|
| Derechos de acceso |
restringido
|
| Access Rights |
restringido
|
| Identificador relacionado |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489821/
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID 21170061
CNPq 301832/2017-0
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| Id de Web of Science |
WOS:000855782400001
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