Repositorio UFRO · Omeka S

Axonal quantification of the white matter association fasciculi in cerebral hemispheres of cow (Bos taurus), pig (Sus scrofa domesticus) and rabbit (Oryctolagus cuniculus)

Primer Autor	Ottone, NE
Co-autores	Guerrero, M. Romero, I Sandoval, C. Gaibor-Pazmino, A. Norona, A. Zurita, V del Sol, M.
Título	Axonal quantification of the white matter association fasciculi in cerebral hemispheres of cow (Bos taurus), pig (Sus scrofa domesticus) and rabbit (Oryctolagus cuniculus)
Editorial	VIA MEDICA
Revista	FOLIA MORPHOLOGICA
Lenguaje	en
Resumen	Background: Cerebral white matter consists mainly of axons surrounded by my-elin sheaths, which are grouped to form association, commissural, and projection fasciculi. The aim of our work was to quantify and compare under the microscope the axons of the white matter association fasciculi in the cerebral hemispheres of cow (Bos taurus), pig (Sus scrofa domesticus) and rabbit (Oryctolagus cuniculus) indirectly by identification of their myelin sheaths.Materials and methods: The samples were taken from 30 cerebral hemispheres: 10 cow, 10 pig and 10 rabbit (15 right and 15 left). They were obtained following a protocol based on the Talairach-Tournoux coordinate system for human and primate brains. The slides were stained with Luxol Fast Blue, observed by optical microscopy, and photographed at 600x. Samples were also prepared for obser-vation in scanning transmission electron microscopy with osmium tetroxide. The myelin sheaths/axons were counted with the ImageJ software.Results: Statistically significant differences in the number of myelin sheaths per 410 mu m2 were found in the inferior and superior longitudinal fasciculi between the left and right hemispheres of cows, with predominance of the right hemisphere, and in the inferior occipitofrontal fasciculus of the rabbit with predominance of the left hemisphere.Conclusions: The use of animal models for experiments in the cerebral fasciculi, especially pig, could give us a greater understanding of the behaviour of demy-elinating and neurodegenerative diseases in humans. (Folia Morphol 2022, 81, 4: 874-883)
Fecha Publicación	2022
Tipo de Recurso	artículo original
Derecho de Acceso	acceso abierto
doi	10.5603/FM.a2021.0116
Formato Recurso	PDF
Palabras Claves	axons cerebral fasciculi histological analysis myelin sheaths white matter
Ubicación del archivo	http://dx.doi.org/10.5603/FM.a2021.0116
Categoría OCDE	Anatomía y Morfología Neurociencias
Materias	axones fascículos cerebrales análisis histológico vainas de mielina materia blanca
Página de inicio (Recomendado-único)	874.0
Página final (Recomendado-único)	883
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY-NC-ND 4.0
Condición de la licencia (Recomendado-repetible)	CC BY-NC-ND 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:000895763700001
ISSN	0015-5659
Tipo de ruta	Verde# dorado
Categoría WOS	Anatomía y Morfología