Repositorio UFRO · Omeka S

Histological changes in lingual striated muscle tissue of human cadavers to estimate the postmortem interval

Primer Autor	Vasquez, Belgica
Co-autores	Guerrero-Urbina, Clivia Fors, Martha Fonseca, Gabriel Rodriguez-Guerrero, Marjorie
Título	Histological changes in lingual striated muscle tissue of human cadavers to estimate the postmortem interval
Editorial	HUMANA PRESS INC
Revista	FORENSIC SCIENCE MEDICINE AND PATHOLOGY
Lenguaje	en
Resumen	Although there are physiological methods to determine the postmortem interval (PMI), interval forensic histopathology can be applied to obtain accuracy. The aim was to describe the histological changes in human lingual striated musculature at different PMI. Seven groups were formed according to increasing PMI of 6, 12, 24, 48, 72, 96 and 120 h postmortem (PM). Each group was made up of 16 samples of tongues from each cadaver. The samples were fixed in buffered formaldehyde at 10% and processed for embedding in paraplast. Section 5 mu m thick were cut and dyed with H&E for analysis. The study was approved by the Bioethics Committee of the Universidad San Francisco de Quito, Ecuador. The histological changes in the striated muscle cells of the tongue were associated with the different PMI. From 6 to 24 h PM, there were initial changes in the cellular and nuclear morphology. At 48 h PM, at least 50% of the samples presented poorly conserved and reduced muscle striations. At 72 h PM, 100% of the cases presented myofibers with altered morphology, cytoplasmic vacuoles (93.75%), edema (68.55%) and pyknosis (93.75%). At 96 and 120 h PM, the myofibers presented pyknotic nuclei, and they were absent in the rest. The changes in the histology of the human lingual striated muscle make it possible to estimate the PMI, either in the early phase (0-72 h) or the late phase (92-120 h). However, further research is needed to verify, refine and expand on these results.
Fecha Publicación	2023
Tipo de Recurso	artículo original
Derecho de Acceso	acceso restringido
doi	10.1007/s12024-022-00495-0
Formato Recurso	PDF
Palabras Claves	Postmortem interval Tongue muscles Histology Forensic medicine
Ubicación del archivo	http://dx.doi.org/10.1007/s12024-022-00495-0
Categoría OCDE	Medicina Legal Patología Ciencia Forense
Materias	Intervalo postmortem Músculos de la lengua Histología Medicina Forense
Página de inicio (Recomendado-único)	16.0
Página final (Recomendado-único)	23
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
Derechos de acceso	restringido
Access Rights	restringido
Id de Web of Science	WOS:000817922500002
ISSN	1547-769X
Categoría WOS	Medicina Legal Patología