Repositorio
Institucional

dos Santos, Vania A. F. F. M.

Coppede, Juliana da Silva

Dias, Nathalia Batista

Soares Pereira, Ana Maria

Palma, Mario Sergio

Furlan, Maysa

Proteome profiling of methyl jasmonate elicitation of Maytenus ilicifolia in vitro roots reveals insights into sesquiterpene pyridine alkaloids

SPRINGER

PLANT CELL TISSUE AND ORGAN CULTURE

en

Key message The study reports the accumulation of two sesquiterpene pyridine alkaloids and the proteins annotated by proteomics on in vitro root of M. ilicifolia as a product of elicitation with MeJA. Maytenus ilicifolia (Celastraceae) is an important medicinal plant widely used in Brazil and a rich source of sesquiterpene pyridine alkaloids (SPAs) with relevant biological activities. They exhibit great chemical diversity and high structural complexity, and show accumulation in very low concentrations. Thus, to determine whether in vitro cultured adventitious roots of M. ilicifolia could provide alternative sources for the production of these compounds, the accumulation of SPAs ilicifoliunine A and aquifoliunine E-I, as well as the time of production and the content of these alkaloids were evaluated using methyl jasmonate (MeJA) as an elicitor. The results showed the accumulation ilicifoliunine A and aquifoliunine E-I and an increase in the contents of these alkaloids when elicited with MeJA at 7 and 28 days, respectively. To characterize and identify the proteins present in the enzymatic system and contribute to the understanding of the metabolic pathways involved in the biosynthesis of SPAs, was carried out in vitro proteomic profiling of adventitious roots elicited and non-elicited with MeJA. The shotgun proteomic analyses led to the identification of important proteins involved in the biosynthetic pathway of SPAs, including germacrene A synthase, beta-eudesmol synthase, and L-aspartate oxidase. Additionally, many proteins that participate in the biosynthesis of other alkaloids (e.g., tropane, pyrrolidine, terpenoid indole, and isoquinoline) and phenylpropanoids have been identified. In conclusion, the study report for the first time, proteins involved in the biosynthetic pathway of SPAs by using the adventitious roots in vitro system of M. ilicifolia and a shotgun proteomic approach.

2022

artículo original

10.1007/s11240-022-02371-9

PDF

Maytenus ilicifolia

Celastraceae

In vitro adventitious roots

Methyl jasmonate

Shotgun proteomic

Sesquiterpene pyridine alkaloids

Biotecnología y microbiología aplicada

Ciencias vegetales

Fitomejoramiento y protección vegetal

Biotecnología agrícola

Maytenus ilicifolia

Celastráceas

Raíces adventicias in vitro

Jasmonato de metilo

Proteómica de escopeta

Alcaloides sesquiterpénicos piridina

versión publicada

hibrida

verde

restringido

restringido

WOS:000879697900001

551.0

563

artículo original

0167-6857

Biotecnología y microbiología aplicada

Ciencias vegetales