Repositorio UFRO · Omeka S

Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies

Primer Autor	Sotomayor-Gerding, Daniela
Co-autores	Miguel Troncoso, Jose Diaz-Riquelme, Katherine Mariana Torres-Obreque, Karin Cumilaf, Juan Yanez, Alejandro J. Rubilar, Monica
Título	Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies
Editorial	MDPI
Revista	POLYMERS
Lenguaje	en
Resumen	The development of fish oral vaccines is of great interest to the aquaculture industry due to the possibility of rapid vaccination of a large number of animals at reduced cost. In a previous study, we evaluated the effect of alginate-encapsulated Piscirickettsia salmonis antigens (AEPSA) incorporated in feed, effectively enhancing the immune response in Atlantic salmon (Salmo salar). In this study, we seek to characterize AEPSA produced by ionic gelation using an aerodynamically assisted jetting (AAJ) system, to optimize microencapsulation efficiency (EE%), to assess microparticle stability against environmental (pH, salinity and temperature) and gastrointestinal conditions, and to evaluate microparticle incorporation in fish feed pellets through micro-CT-scanning. The AAJ system was effective in obtaining small microparticles (d < 20 mu m) with a high EE% (97.92%). Environmental conditions (pH, salinity and temperature) generated instability in the microparticles, triggering protein release. 62.42% of the protein content was delivered at the intestinal level after in vitro digestion. Finally, micro-CT-scanning images confirmed microparticle incorporation in fish feed pellets. In conclusion, the AAJ system is effective at encapsulating P. salmonis antigens in alginate with a high EE% and a size small enough to be incorporated in fish feed and produce an oral vaccine.
Fecha Publicación	2022
Tipo de Recurso	artículo original
doi	10.3390/polym14235115
Formato Recurso	PDF
Palabras Claves	Piscirickettsia salmonis oral vaccine alginate fish feed vacuum coating particle size encapsulation efficiency antigen stability Micro-CT-scanning
Ubicación del archivo	http://dx.doi.org/10.3390/polym14235115
Categoría OCDE	Ciencia de los polímeros Polímeros Biotecnología de Diagnóstico (Chips de ADN y dispositivos biosensores) en manejo ambiental
Materias	Piscirickettsia salmonis vacuna oral alginato alimento para peces recubrimiento al vacío tamaño de partícula eficiencia de encapsulación antígeno estabilidad Escaneo micro-CT
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:000896388900001
Tipo de ruta	verde# dorado
Categoría WOS	Ciencia de los polímeros