Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies

Primer Autor
Sotomayor-Gerding, Daniela
Co-autores
Miguel Troncoso, Jose
Diaz-Riquelme, Katherine
Mariana Torres-Obreque, Karin
Cumilaf, Juan
Yanez, Alejandro J.
Rubilar, Monica
Título
Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies
Editorial
MDPI
Revista
POLYMERS
Lenguaje
en
Resumen
The development of fish oral vaccines is of great interest to the aquaculture industry due to the possibility of rapid vaccination of a large number of animals at reduced cost. In a previous study, we evaluated the effect of alginate-encapsulated Piscirickettsia salmonis antigens (AEPSA) incorporated in feed, effectively enhancing the immune response in Atlantic salmon (Salmo salar). In this study, we seek to characterize AEPSA produced by ionic gelation using an aerodynamically assisted jetting (AAJ) system, to optimize microencapsulation efficiency (EE%), to assess microparticle stability against environmental (pH, salinity and temperature) and gastrointestinal conditions, and to evaluate microparticle incorporation in fish feed pellets through micro-CT-scanning. The AAJ system was effective in obtaining small microparticles (d < 20 mu m) with a high EE% (97.92%). Environmental conditions (pH, salinity and temperature) generated instability in the microparticles, triggering protein release. 62.42% of the protein content was delivered at the intestinal level after in vitro digestion. Finally, micro-CT-scanning images confirmed microparticle incorporation in fish feed pellets. In conclusion, the AAJ system is effective at encapsulating P. salmonis antigens in alginate with a high EE% and a size small enough to be incorporated in fish feed and produce an oral vaccine.
Fecha Publicación
2022
Tipo de Recurso
artículo original
doi
10.3390/polym14235115
Formato Recurso
PDF
Palabras Claves
Piscirickettsia salmonis
oral vaccine
alginate
fish feed
vacuum coating
particle size
encapsulation efficiency
antigen
stability
Micro-CT-scanning
Ubicación del archivo
http://dx.doi.org/10.3390/polym14235115
Categoría OCDE
Ciencia de los polímeros
Polímeros
Biotecnología de Diagnóstico (Chips de ADN y dispositivos biosensores) en manejo ambiental
Materias
Piscirickettsia salmonis
vacuna oral
alginato
alimento para peces
recubrimiento al vacío
tamaño de partícula
eficiencia de encapsulación
antígeno
estabilidad
Escaneo micro-CT
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
versión publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:000896388900001
Tipo de ruta
verde# dorado
Categoría WOS
Ciencia de los polímeros
Colecciones
Colección Publicaciones Científicas
Colección ANID
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