Repositorio UFRO · Omeka S

Flow cytometry: a tool for understanding the behaviour of polyhydroxyalkanoate accumulators

Primer Autor	Cea, Mara
Co-autores	Gonzalez, Karina Salinas, Alejandro Pinto, Fernanda Navia, Rodrigo Liu, Shijie
Título	Flow cytometry: a tool for understanding the behaviour of polyhydroxyalkanoate accumulators
Editorial	SPRINGER
Revista	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Lenguaje	en
Resumen	The use of mixed microbial cultures (MMCs) is seen as an attractive strategy for polyhydroxyalkanoate (PHA) production. In order to optimize the MMC-PHA production process, tools are required to improve our understanding of the physiological state of the PHA-storing microorganisms within the MMC. In the present study, we explored the use of flow cytometry to analyse the metabolic state and polyhydroxybutyrate (PHB) content of the microorganisms from an MMC-PHA production process. A sequencing batch reactor under a feast and famine regime was used to enrich an MMC with PHB-storing microorganisms. Interestingly, once the PHB-storing microorganisms are selected, the level of PHB accumulation depends largely on the metabolic state of these microorganisms and not exclusively on the consortium composition. These results demonstrate that flow cytometry is a powerful tool to help to understand the PHA storage response of an MMC-PHA production process.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.1007/s00253-022-12318-x
Formato Recurso	PDF
Palabras Claves	Polyhydroxyalkanoates Mixed microbial cultures Bioreactors Flow cytometry
Ubicación del archivo	http://dx.doi.org/10.1007/s00253-022-12318-x
Categoría OCDE	Biotecnología y Microbiología Aplicada Biotecnología Ambiental Microbiología
Materias	polihidroxialcanoatos Cultivos microbianos mixtos Biorreactores Citometría de flujo
Página de inicio (Recomendado-único)	581.0
Página final (Recomendado-único)	590
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
Derechos de acceso	metadata
Access Rights	metadata
Id de Web of Science	WOS:000899766600001
ISSN	0175-7598
Categoría WOS	Biotecnología y Microbiología Aplicada
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID 21191476 ANID ACT 172128 ANID FONDECYT 3200748