Flow cytometry: a tool for understanding the behaviour of polyhydroxyalkanoate accumulators
| Primer Autor |
Cea, Mara
|
| Co-autores |
Gonzalez, Karina
Salinas, Alejandro
Pinto, Fernanda
Navia, Rodrigo
Liu, Shijie
|
| Título |
Flow cytometry: a tool for understanding the behaviour of polyhydroxyalkanoate accumulators
|
| Editorial |
SPRINGER
|
| Revista |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
|
| Lenguaje |
en
|
| Resumen |
The use of mixed microbial cultures (MMCs) is seen as an attractive strategy for polyhydroxyalkanoate (PHA) production. In order to optimize the MMC-PHA production process, tools are required to improve our understanding of the physiological state of the PHA-storing microorganisms within the MMC. In the present study, we explored the use of flow cytometry to analyse the metabolic state and polyhydroxybutyrate (PHB) content of the microorganisms from an MMC-PHA production process. A sequencing batch reactor under a feast and famine regime was used to enrich an MMC with PHB-storing microorganisms. Interestingly, once the PHB-storing microorganisms are selected, the level of PHB accumulation depends largely on the metabolic state of these microorganisms and not exclusively on the consortium composition. These results demonstrate that flow cytometry is a powerful tool to help to understand the PHA storage response of an MMC-PHA production process.
|
| Fecha Publicación |
2023
|
| Tipo de Recurso |
artículo original
|
| doi |
10.1007/s00253-022-12318-x
|
| Formato Recurso |
PDF
|
| Palabras Claves |
Polyhydroxyalkanoates
Mixed microbial cultures
Bioreactors
Flow cytometry
|
| Ubicación del archivo | |
| Categoría OCDE |
Biotecnología y Microbiología Aplicada
Biotecnología Ambiental
Microbiología
|
| Materias |
polihidroxialcanoatos
Cultivos microbianos mixtos
Biorreactores
Citometría de flujo
|
| Página de inicio (Recomendado-único) |
581.0
|
| Página final (Recomendado-único) |
590
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| Derechos de acceso |
metadata
|
| Access Rights |
metadata
|
| Id de Web of Science |
WOS:000899766600001
|
| ISSN |
0175-7598
|
| Categoría WOS |
Biotecnología y Microbiología Aplicada
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID 21191476
ANID ACT 172128
ANID FONDECYT 3200748
|
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