Repositorio UFRO · Omeka S

Nitrogen Sources Affect the Long-Chain Polyunsaturated Fatty Acids Content in Thraustochytrium sp. RT2316-16

Primer Autor	Shene, Carolina
Co-autores	Valdebenito, Diego Urrutia, Sebastian Leyton, Allison Chisti, Yusuf Asenjo, Juan A.
Título	Nitrogen Sources Affect the Long-Chain Polyunsaturated Fatty Acids Content in Thraustochytrium sp. RT2316-16
Editorial	MDPI
Revista	MARINE DRUGS
Lenguaje	en
Resumen	The psychrophilic marine microorganism Thraustochytrium sp. RT2316-16 can produce carotenoids as well as lipids containing the omega-3 polyunsaturated fatty acids (PUFA) eicosapentaenoic acid and docosahexaenoic acid. This work reports on the effects of the composition of the culture medium, including certain amino acids, on growth and lipid synthesis by RT2316-16. Compared with the culture on glutamate, the use of lysine, alanine, or serine, increased the content of the omega-3 PUFA in total lipids. In the media that contained yeast extract, glutamate, and glucose, lipid accumulation occurred when organic ammonium was exhausted earlier than glucose. In contrast, lipid mobilization was promoted if glucose was exhausted while organic ammonium (supplied by yeast extract and glutamate) remained in the medium. The total content of carotenoids in the lipid-free biomass decreased during the first 12 to 24 h of culture, simultaneously with a decrease in the total lipid content of the biomass. The experimental data suggested a possible interrelationship between the metabolism of carotenoids and lipids. A high content of omega-3 PUFA in the total lipids could be obtained by growing the thraustochytrid in a medium with a low glucose concentration (6 g L-1) and a high concentration of organic nitrogen (yeast extract 12 g L-1, glutamate 1.06 g L-1), after glucose was exhausted. These observations may guide the development of a strategy to enhance omega-3 PUFA in the biomass.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/md21010015
Formato Recurso	PDF
Palabras Claves	Thraustochytrium sp carotenoids omega-3 polyunsaturated fatty acids microbial lipids amino acids
Ubicación del archivo	http://dx.doi.org/10.3390/md21010015
Categoría OCDE	Farmacología y farmacia
Materias	Thraustochytrium sp carotenoides ácidos grasos poliinsaturados omega-3 lípidos microbianos aminoácidos
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:000917610300001
Tipo de ruta	verde# dorado
Categoría WOS	Farmacología y farmacia
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID FONDECYT 1200642 CeBiB FB-0001 ANID FONDECYT 21181288