Nitrogen Sources Affect the Long-Chain Polyunsaturated Fatty Acids Content in Thraustochytrium sp. RT2316-16
| Primer Autor |
Shene, Carolina
|
| Co-autores |
Valdebenito, Diego
Urrutia, Sebastian
Leyton, Allison
Chisti, Yusuf
Asenjo, Juan A.
|
| Título |
Nitrogen Sources Affect the Long-Chain Polyunsaturated Fatty Acids Content in Thraustochytrium sp. RT2316-16
|
| Editorial |
MDPI
|
| Revista |
MARINE DRUGS
|
| Lenguaje |
en
|
| Resumen |
The psychrophilic marine microorganism Thraustochytrium sp. RT2316-16 can produce carotenoids as well as lipids containing the omega-3 polyunsaturated fatty acids (PUFA) eicosapentaenoic acid and docosahexaenoic acid. This work reports on the effects of the composition of the culture medium, including certain amino acids, on growth and lipid synthesis by RT2316-16. Compared with the culture on glutamate, the use of lysine, alanine, or serine, increased the content of the omega-3 PUFA in total lipids. In the media that contained yeast extract, glutamate, and glucose, lipid accumulation occurred when organic ammonium was exhausted earlier than glucose. In contrast, lipid mobilization was promoted if glucose was exhausted while organic ammonium (supplied by yeast extract and glutamate) remained in the medium. The total content of carotenoids in the lipid-free biomass decreased during the first 12 to 24 h of culture, simultaneously with a decrease in the total lipid content of the biomass. The experimental data suggested a possible interrelationship between the metabolism of carotenoids and lipids. A high content of omega-3 PUFA in the total lipids could be obtained by growing the thraustochytrid in a medium with a low glucose concentration (6 g L-1) and a high concentration of organic nitrogen (yeast extract 12 g L-1, glutamate 1.06 g L-1), after glucose was exhausted. These observations may guide the development of a strategy to enhance omega-3 PUFA in the biomass.
|
| Fecha Publicación |
2023
|
| Tipo de Recurso |
artículo original
|
| doi |
10.3390/md21010015
|
| Formato Recurso |
PDF
|
| Palabras Claves |
Thraustochytrium sp
carotenoids
omega-3 polyunsaturated fatty acids
microbial lipids
amino acids
|
| Ubicación del archivo | |
| Categoría OCDE |
Farmacología y farmacia
|
| Materias |
Thraustochytrium sp
carotenoides
ácidos grasos poliinsaturados omega-3
lípidos microbianos
aminoácidos
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| License |
CC BY 4.0
|
| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
|
| Access Rights |
acceso abierto
|
| Id de Web of Science |
WOS:000917610300001
|
| Tipo de ruta |
verde# dorado
|
| Categoría WOS |
Farmacología y farmacia
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID FONDECYT 1200642
CeBiB FB-0001
ANID FONDECYT 21181288
|
cienciaabierta.ufro.cl
ciencia.abierta@ufrontera.cl