Repositorio UFRO · Omeka S

Ex Situ Conservation of Atriplex taltalensis IM Johnst. via In Vitro Culturing of Its Axillary Shoots

Primer Autor	Paneque, Manuel
Co-autores	Munoz-Alcayaga, Carolina Soto, Jorge Roman-Figueroa, Celian
Título	Ex Situ Conservation of Atriplex taltalensis IM Johnst. via In Vitro Culturing of Its Axillary Shoots
Editorial	MDPI
Revista	DIVERSITY-BASEL
Lenguaje	en
Resumen	Atriplex taltalensis is an endangered shrub endemic to northern Chile. Sparse populations of this species can be found in areas with extreme edaphoclimatic conditions in the Atacama desert, and its seeds have a poor germination rate. Consequently, mass-cultivating it can be challenging. In this study, the vegetative propagation mechanisms of A. taltalensis were evaluated using an in vitro culture to aid in their conservation. A. taltalensis shoot explants were treated with two phytoregulators, indole-3-butyric acid (IBA) and 6-benzylaminopurine (BAP), to assess the morphogenic responses and their effects on the propagation of the species, based on shoot multiplication, elongation, and rooting, through subculturing. During multiplication, the treatment with IBA alone efficiently promoted explant elongation, lateral root formation, and axillary shoot proliferation, allowing for the rapid development of shoots into whole plants. Alternatively, treatment with IBA and BAP in combination stimulated the proliferation of basal shoots with little elongation and rooting and promoted shoot hyperhydricity at 0.25-1 mg L-1 BAP concentrations. Thus, we conclude that A. taltalensis propagation is viable through in vitro plant tissue culture using a rapid axillary shoot multiplication system, and this method could aid in the conservation of this species through in vitro propagation and rescue programs.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/d15010013
Formato Recurso	PDF
Palabras Claves	arid and semi-arid environments micropropagation ex situ conservation dry land in vitro culturing endemic plants
Ubicación del archivo	http://dx.doi.org/10.3390/d15010013
Categoría OCDE	Biodiversidad y Conservación Ciencias Ambientales y Ecología
Materias	ambientes áridos y semiáridos micropropagación conservación ex situ tierra seca cultivo in vitro plantas endémicas
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:000915050700001
Tipo de ruta	verde# dorado
Categoría WOS	Biodiversidad y Conservación Ciencias Ambientales y Ecología