Ex Situ Conservation of Atriplex taltalensis IM Johnst. via In Vitro Culturing of Its Axillary Shoots
| Primer Autor |
Paneque, Manuel
|
| Co-autores |
Munoz-Alcayaga, Carolina
Soto, Jorge
Roman-Figueroa, Celian
|
| Título |
Ex Situ Conservation of Atriplex taltalensis IM Johnst. via In Vitro Culturing of Its Axillary Shoots
|
| Editorial |
MDPI
|
| Revista |
DIVERSITY-BASEL
|
| Lenguaje |
en
|
| Resumen |
Atriplex taltalensis is an endangered shrub endemic to northern Chile. Sparse populations of this species can be found in areas with extreme edaphoclimatic conditions in the Atacama desert, and its seeds have a poor germination rate. Consequently, mass-cultivating it can be challenging. In this study, the vegetative propagation mechanisms of A. taltalensis were evaluated using an in vitro culture to aid in their conservation. A. taltalensis shoot explants were treated with two phytoregulators, indole-3-butyric acid (IBA) and 6-benzylaminopurine (BAP), to assess the morphogenic responses and their effects on the propagation of the species, based on shoot multiplication, elongation, and rooting, through subculturing. During multiplication, the treatment with IBA alone efficiently promoted explant elongation, lateral root formation, and axillary shoot proliferation, allowing for the rapid development of shoots into whole plants. Alternatively, treatment with IBA and BAP in combination stimulated the proliferation of basal shoots with little elongation and rooting and promoted shoot hyperhydricity at 0.25-1 mg L-1 BAP concentrations. Thus, we conclude that A. taltalensis propagation is viable through in vitro plant tissue culture using a rapid axillary shoot multiplication system, and this method could aid in the conservation of this species through in vitro propagation and rescue programs.
|
| Fecha Publicación |
2023
|
| Tipo de Recurso |
artículo original
|
| doi |
10.3390/d15010013
|
| Formato Recurso |
PDF
|
| Palabras Claves |
arid and semi-arid environments
micropropagation
ex situ conservation
dry land
in vitro culturing
endemic plants
|
| Ubicación del archivo | |
| Categoría OCDE |
Biodiversidad y Conservación
Ciencias Ambientales y Ecología
|
| Materias |
ambientes áridos y semiáridos
micropropagación
conservación ex situ
tierra seca
cultivo in vitro
plantas endémicas
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| License |
CC BY 4.0
|
| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
|
| Access Rights |
acceso abierto
|
| Id de Web of Science |
WOS:000915050700001
|
| Tipo de ruta |
verde# dorado
|
| Categoría WOS |
Biodiversidad y Conservación
Ciencias Ambientales y Ecología
|
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