Identification and ligand binding of a chemosensory protein from sea louse Caligus rogercresseyi (Crustacea: Copepoda)

Primer Autor
Mutis, Ana
Co-autores
Pino, Jorge
Godoy, Ricardo
Venthur, Herbert
Larama, Giovanni
Quiroz, Andres
Título
Identification and ligand binding of a chemosensory protein from sea louse Caligus rogercresseyi (Crustacea: Copepoda)
Editorial
ELSEVIER SCIENCE INC
Revista
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Lenguaje
en
Resumen
Caligus rogercresseyi is an ectoparasitic copepod that negatively affects the salmon farming industry, causing economic losses. To use phytochemicals as feed additives, or other chemicals that could elicit behavioral re-sponses in C. rogercresseyi, the chemosensory recognition process is crucial. Therefore, to establish how C. rogercresseyi recognizes glucosinolates and their derivates isothiocyanates, a chemosensory protein (CSP) described as specific carrier of these chemicals in sea louse (CrogCSP) was identified in this study. The recom-binant CSP and its selectivity against different chemical compounds was tested by fluorescence binding assays. Phylogenetic analysis revealed a close relationship among CrogCSP and other reported CSPs. Our results indicate that phenyl isothiocyanate and isophorone exhibited dissociation constants of 4.17 and 4.28 mu M of Ki, respec-tively, indicating affinity over other chemicals, such as fatty acids and sinigrin. Structural findings suggest a unique binding site capable of accept several types of chemicals, similar to what has been reported for crys-tallized insect CSPs. Finally, this study lays the foundation for a deeper understanding of CSPs in crustaceans and especially in C. rogercresseyi. Likewise, the identification of chemosensory proteins could serve as the first step towards novel semiochemicals discovery to being applied in the sea louse controlling.
Fecha Publicación
2023
Tipo de Recurso
artículo original
doi
10.1016/j.cbpb.2023.110830
Formato Recurso
PDF
Palabras Claves
Chemosensory proteins (CSPs)
Glucosinolates
Olfaction
Reverse chemical ecology
Sea louse
Ubicación del archivo
http://dx.doi.org/10.1016/j.cbpb.2023.110830
Categoría OCDE
Bioquímica y biología molecular
Zoología
Materias
Proteínas quimiosensoriales (CSP)
Glucosinolatos
Olfato
Ecología química inversa
piojo de mar
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
versión publicada
Derechos de acceso
metadata
Access Rights
metadata
Id de Web of Science
WOS:000993623000001
ISSN
1096-4959
Categoría WOS
Bioquímica y biología molecular
Zoología
Colecciones
Colección Publicaciones Científicas
Colección ANID
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