Repositorio UFRO · Omeka S

Identification and ligand binding of a chemosensory protein from sea louse Caligus rogercresseyi (Crustacea: Copepoda)

Primer Autor	Mutis, Ana
Co-autores	Pino, Jorge Godoy, Ricardo Venthur, Herbert Larama, Giovanni Quiroz, Andres
Título	Identification and ligand binding of a chemosensory protein from sea louse Caligus rogercresseyi (Crustacea: Copepoda)
Editorial	ELSEVIER SCIENCE INC
Revista	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Lenguaje	en
Resumen	Caligus rogercresseyi is an ectoparasitic copepod that negatively affects the salmon farming industry, causing economic losses. To use phytochemicals as feed additives, or other chemicals that could elicit behavioral re-sponses in C. rogercresseyi, the chemosensory recognition process is crucial. Therefore, to establish how C. rogercresseyi recognizes glucosinolates and their derivates isothiocyanates, a chemosensory protein (CSP) described as specific carrier of these chemicals in sea louse (CrogCSP) was identified in this study. The recom-binant CSP and its selectivity against different chemical compounds was tested by fluorescence binding assays. Phylogenetic analysis revealed a close relationship among CrogCSP and other reported CSPs. Our results indicate that phenyl isothiocyanate and isophorone exhibited dissociation constants of 4.17 and 4.28 mu M of Ki, respec-tively, indicating affinity over other chemicals, such as fatty acids and sinigrin. Structural findings suggest a unique binding site capable of accept several types of chemicals, similar to what has been reported for crys-tallized insect CSPs. Finally, this study lays the foundation for a deeper understanding of CSPs in crustaceans and especially in C. rogercresseyi. Likewise, the identification of chemosensory proteins could serve as the first step towards novel semiochemicals discovery to being applied in the sea louse controlling.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.1016/j.cbpb.2023.110830
Formato Recurso	PDF
Palabras Claves	Chemosensory proteins (CSPs) Glucosinolates Olfaction Reverse chemical ecology Sea louse
Ubicación del archivo	http://dx.doi.org/10.1016/j.cbpb.2023.110830
Categoría OCDE	Bioquímica y biología molecular Zoología
Materias	Proteínas quimiosensoriales (CSP) Glucosinolatos Olfato Ecología química inversa piojo de mar
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
Derechos de acceso	metadata
Access Rights	metadata
Id de Web of Science	WOS:000993623000001
ISSN	1096-4959
Categoría WOS	Bioquímica y biología molecular Zoología