Repositorio
Institucional

Hermosilla, Edward

Diaz, Marcela

Vera, Joelis

Contreras, Maria Jose

Leal, Karla

Salazar, Rodrigo

Barrientos, Leticia

Tortella, Gonzalo

Rubilar, Olga

Synthesis of Antimicrobial Chitosan-Silver Nanoparticles Mediated by Reusable Chitosan Fungal Beads

MDPI

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

en

Nanoparticles, especially silver nanoparticles (Ag NPs), have gained significant attention in recent years as potential alternatives to traditional antibiotics for treating infectious diseases due to their ability to inhibit the growth of microorganisms effectively. Ag NPs can be synthesized using fungi extract, but the method is not practical for large-scale production due to time and biomass limitations. In this study, we explore the use of chitosan to encapsulate the mycelia of the white-rot fungus Stereum hirsutum and form chitosan fungal beads for use in multiple extractions and nanoparticle synthesis. The resulting nanoparticles were characterized using various techniques, including UV-vis spectrophotometry, transmission electron microscopy, dynamic light scattering, and X-ray diffraction analysis. The analysis revealed that the synthesized nanoparticles were composed of chitosan-silver nanoparticles (CS-Ag NPs) with a size of 25 nm. The chitosan fungal beads were reused in three extractions and nanoparticle synthesis before they lost their ability to produce CS-Ag NPs. The CS-Ag NPs showed potent antimicrobial activity against phytopathogenic and human pathogenic microorganisms, including Pseudomonas syringae, Escherichia coli, Staphylococcus aureus, and Candida albicans, with minimum inhibitory concentrations of 1.5, 1.6, 3.1, and 4 mu g/mL, respectively. The antimicrobial activity of CS-Ag NPs was from 2- to 40-fold higher than Ag NPs synthesized using an aqueous extract of unencapsulated fungal biomass. The CS-Ag NPs were most effective at a pH of five regarding the antimicrobial activity. These results suggest that the chitosan fungal beads may be a promising alternative for the sustainable and cost-effective synthesis of CS-Ag NPs with improved antimicrobial activity.

2023

artículo original

10.3390/ijms24032318

PDF

nanoparticle synthesis

chitosan-silver nanoparticles

reusable fungal biomass

antimicrobial activity

Bioquímica y biología molecular

Química

síntesis de nanopartículas

nanopartículas de quitosano-plata

biomasa fúngica reutilizable

actividad antimicrobiana

artículo original

versión publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

WOS:000932567400001

verde# dorado

Bioquímica y biología molecular

Química

ANID-FONDECYT 1191089

ANID-FONDECYT 3190922

ANID-FAPESP 2018/08194-2

ANID-FONDAP 15130015

ANID FONDECYT 1191089

ANID FONDECYT 3190922

ANID FAPESP 2018/08194-2

ANID FONDAP 15130015