Structure and Functional Properties of Bacterial Communities in Surface Sediments of the Recently Declared Nutrient-Saturated Lake Villarrica in Southern Chile
| Primer Autor |
Jorquera, Milko A.
|
| Co-autores |
Campos, Marco A.
Zhang, Qian
Acuna, Jacquelinne J.
Rilling, Joaquin I.
Ruiz, Tay
Carrazana, Elizabeth
Reyno, Cristobal
Hollenback, Anthony
Gray, Katelyn
Jaisi, Deb P.
Ogram, Andrew
Bai, Junhong
Zhang, Ling
Xiao, Rong
Elias, Mikael
Sadowsky, Michael J.
Hu, Jingming
|
| Título |
Structure and Functional Properties of Bacterial Communities in Surface Sediments of the Recently Declared Nutrient-Saturated Lake Villarrica in Southern Chile
|
| Editorial |
SPRINGER
|
| Revista |
MICROBIAL ECOLOGY
|
| Lenguaje |
en
|
| Resumen |
Lake Villarrica, one of Chile's main freshwater water bodies, was recently declared a nutrient-saturated lake due to increased phosphorus (P) and nitrogen (N) levels. Although a decontamination plan based on environmental parameters is being established, it does not consider microbial parameters. Here, we conducted high-throughput DNA sequencing and quantitative polymerase chain reaction (qPCR) analyses to reveal the structure and functional properties of bacterial communities in surface sediments collected from sites with contrasting anthropogenic pressures in Lake Villarrica. Alpha diversity revealed an elevated bacterial richness and diversity in the more anthropogenized sediments. The phylum Proteobacteria, Bacteroidetes, Acidobacteria, and Actinobacteria dominated the community. The principal coordinate analysis (PCoA) and redundancy analysis (RDA) showed significant differences in bacterial communities of sampling sites. Predicted functional analysis showed that N cycling functions (e.g., nitrification and denitrification) were significant. The microbial co-occurrence networks analysis suggested Chitinophagaceae, Caldilineaceae, Planctomycetaceae, and Phycisphaerae families as keystone taxa. Bacterial functional genes related to P (phoC, phoD, and phoX) and N (nifH and nosZ) cycling were detected in all samples by qPCR. In addition, an RDA related to N and P cycling revealed that physicochemical properties and functional genes were positively correlated with several nitrite-oxidizing, ammonia-oxidizing, and N-fixing bacterial genera. Finally, denitrifying gene (nosZ) was the most significant factor influencing the topological characteristics of co-occurrence networks and bacterial interactions. Our results represent one of a few approaches to elucidate the structure and role of bacterial communities in Chilean lake sediments, which might be helpful in conservation and decontamination plans.
|
| Fecha Publicación |
2023
|
| Tipo de Recurso |
artículo original
|
| doi |
10.1007/s00248-023-02173-2
|
| Formato Recurso |
PDF
|
| Palabras Claves |
Bacterial communities
Bacterial diversity
Functional genes
Lake sediments
Nutrients
Community structure
|
| Ubicación del archivo | |
| Categoría OCDE |
Ciencias ambientales y ecología
Biología marina y de agua dulce
Microbiología
|
| Materias |
Comunidades bacterianas
Diversidad bacteriana
Genes funcionales
Sedimentos lacustres
Nutrientes
Estructura comunitaria
|
| Página de inicio (Recomendado-único) |
1513.0
|
| Página final (Recomendado-único) |
1533
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| Derechos de acceso |
metadata
|
| Access Rights |
metadata
|
| Id de Web of Science |
WOS:000929402900001
|
| ISSN |
0095-3628
|
| Categoría WOS |
Ciencias ambientales y ecología
Biología marina y de agua dulce
Microbiología
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID NSFC190012
NSFC 51961125201
ANID REDES190079
ANID-FONDECYT 1201386
ANID-FONDECYT 1221228
NSFC 42077386
SATREPS JPMJSA1705
ANID FONDECYT 1201386
ANID FONDECYT 1221228
|
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