Repositorio
Institucional

Jorquera, Milko A.

Campos, Marco A.

Zhang, Qian

Acuna, Jacquelinne J.

Rilling, Joaquin I.

Ruiz, Tay

Carrazana, Elizabeth

Reyno, Cristobal

Hollenback, Anthony

Gray, Katelyn

Jaisi, Deb P.

Ogram, Andrew

Bai, Junhong

Zhang, Ling

Xiao, Rong

Elias, Mikael

Sadowsky, Michael J.

Hu, Jingming

Structure and Functional Properties of Bacterial Communities in Surface Sediments of the Recently Declared Nutrient-Saturated Lake Villarrica in Southern Chile

SPRINGER

MICROBIAL ECOLOGY

en

Lake Villarrica, one of Chile's main freshwater water bodies, was recently declared a nutrient-saturated lake due to increased phosphorus (P) and nitrogen (N) levels. Although a decontamination plan based on environmental parameters is being established, it does not consider microbial parameters. Here, we conducted high-throughput DNA sequencing and quantitative polymerase chain reaction (qPCR) analyses to reveal the structure and functional properties of bacterial communities in surface sediments collected from sites with contrasting anthropogenic pressures in Lake Villarrica. Alpha diversity revealed an elevated bacterial richness and diversity in the more anthropogenized sediments. The phylum Proteobacteria, Bacteroidetes, Acidobacteria, and Actinobacteria dominated the community. The principal coordinate analysis (PCoA) and redundancy analysis (RDA) showed significant differences in bacterial communities of sampling sites. Predicted functional analysis showed that N cycling functions (e.g., nitrification and denitrification) were significant. The microbial co-occurrence networks analysis suggested Chitinophagaceae, Caldilineaceae, Planctomycetaceae, and Phycisphaerae families as keystone taxa. Bacterial functional genes related to P (phoC, phoD, and phoX) and N (nifH and nosZ) cycling were detected in all samples by qPCR. In addition, an RDA related to N and P cycling revealed that physicochemical properties and functional genes were positively correlated with several nitrite-oxidizing, ammonia-oxidizing, and N-fixing bacterial genera. Finally, denitrifying gene (nosZ) was the most significant factor influencing the topological characteristics of co-occurrence networks and bacterial interactions. Our results represent one of a few approaches to elucidate the structure and role of bacterial communities in Chilean lake sediments, which might be helpful in conservation and decontamination plans.

2023

artículo original

10.1007/s00248-023-02173-2

PDF

Bacterial communities

Bacterial diversity

Functional genes

Lake sediments

Nutrients

Community structure

Ciencias ambientales y ecología

Biología marina y de agua dulce

Microbiología

Comunidades bacterianas

Diversidad bacteriana

Genes funcionales

Sedimentos lacustres

Nutrientes

Estructura comunitaria

1513.0

1533

artículo original

versión publicada

metadata

metadata

WOS:000929402900001

0095-3628

Ciencias ambientales y ecología

Biología marina y de agua dulce

Microbiología

ANID NSFC190012

NSFC 51961125201

ANID REDES190079

ANID-FONDECYT 1201386

ANID-FONDECYT 1221228

NSFC 42077386

SATREPS JPMJSA1705

ANID FONDECYT 1201386

ANID FONDECYT 1221228