Cholesterol-Related lncRNAs as Response Predictors of Atorvastatin Treatment in Chilean Hypercholesterolemic Patients: A Pilot Study
| Primer Autor |
Salazar, Luis A.
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| Co-autores |
Paez, Isis
Prado, Yalena
Loren, Pia
Ubilla, Carmen G.
Rodriguez, Nelia
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| Título |
Cholesterol-Related lncRNAs as Response Predictors of Atorvastatin Treatment in Chilean Hypercholesterolemic Patients: A Pilot Study
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| Editorial |
MDPI
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| Revista |
BIOMEDICINES
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| Lenguaje |
en
|
| Resumen |
Statins are currently the treatment of choice for hypercholesterolemia. However, wide interindividual variability has been observed in the response to treatment. Recent studies have reported the role of lncRNAs in the metabolism of lipids, nevertheless, there are few studies to date that show their role in the response to treatment with statins. Thus, the aim of this study was to assess the levels of expression of three lncRNAs (RP1-13D10.2, MANTIS, lncHR1) associated with genes involved in cholesterol homeostasis in leukocyte cells of hypercholesterolemic patients after treatment with atorvastatin and compare them with levels in subjects with normal cholesterol levels. A secondary aim was to assess the levels of expression in monocytic THP-1 cells differentiated to macrophages. The study included 20 subjects with normal cholesterol (NC) levels and 20 individuals with hypercholesterolemia (HC). The HC patients were treated with atorvastatin (20 mg/day/4 weeks). THP-1 cells were differentiated to macrophages with PMA and treated with different doses of atorvastatin for 24 h. Expression of lncRNAs was determined by RT-qPCR. The lncRNAs RP1-13D10.2 (p < 0.0001), MANTIS (p = 0.0013) and lncHR1 (p < 0.0001) presented increased expression in HC subjects compared with NC subjects. Furthermore, atorvastatin had a negative regulatory effect on the expression of lncHR1 (p < 0.0001) in HC subjects after treatment. In vitro, all the lncRNAs showed significant differences in expression after atorvastatin treatment. Our findings show that the lncRNAs tested present differential expression in HC patients and play a role in the variability reported in the response to atorvastatin treatment. Further research is needed to clarify the biological impact of these lncRNAs on cholesterol homeostasis and treatment with statins.
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| Fecha Publicación |
2023
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| Tipo de Recurso |
artículo original
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| doi |
10.3390/biomedicines11030742
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| Formato Recurso |
PDF
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| Palabras Claves |
lncRNAs
atorvastatin
normal cholesterol
hypercholesterolemia
THP-1
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| Ubicación del archivo | |
| Categoría OCDE |
Bioquímica y biología molecular
Investigación y medicina experimental
Farmacología y farmacia
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| Materias |
ARNnc
atorvastatina
colesterol normal
hipercolesterolemia
THP-1
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| Identificador del recurso (Mandatado-único) |
artículo original
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| Versión del recurso (Recomendado-único) |
versión publicada
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| License |
CC BY 4.0
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| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
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| Access Rights |
acceso abierto
|
| Id de Web of Science |
WOS:000954670200001
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| Tipo de ruta |
verde# dorado
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| Categoría WOS |
Bioquímica y biología molecular
Investigación y medicina experimental
Farmacología y farmacia
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID-FONDECYT 1171765
ANID FONDECYT 1171765
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cienciaabierta.ufro.cl
ciencia.abierta@ufrontera.cl