Repositorio UFRO · Omeka S

Protist-Lactic Acid Bacteria Co-Culture as a Strategy to Bioaccumulate Polyunsaturated Fatty Acids in the Protist Aurantiochytrium sp. T66

Primer Autor	Viscardi, Sharon
Co-autores	Marileo, Luis Acuna, Jacqueline Rilling, Joaquin Diaz, Paola Langellotti, Antonio Luca Russo, Giovanni Luca Barra, Patricio Javier Dantagnan, Patricio
Título	Protist-Lactic Acid Bacteria Co-Culture as a Strategy to Bioaccumulate Polyunsaturated Fatty Acids in the Protist Aurantiochytrium sp. T66
Editorial	MDPI
Revista	MARINE DRUGS
Lenguaje	en
Resumen	Thraustochytrids are aquatic unicellular protists organisms that represent an important reservoir of a wide range of bioactive compounds, such as essential polyunsaturated fatty acids (PUFAs) such as arachidonic acid (ARA), docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), which are involved in the regulation of the immune system. In this study, we explore the use of co-cultures of Aurantiochytrium sp. and bacteria as a biotechnological tool capable of stimulating PUFA bioaccumulation. In particular, the co-culture of lactic acid bacteria and the protist Aurantiochytrium sp. T66 induce PUFA bioaccumulation, and the lipid profile was evaluated in cultures at different inoculation times, with two different strains of lactic acid bacteria capable of producing the tryptophan dependent auxins, and one strain of Azospirillum sp., as a reference for auxin production. Our results showed that the Lentilactobacillus kefiri K6.10 strain inoculated at 72 h gives the best PUFA content (30.89 mg g(-1) biomass) measured at 144 h of culture, three times higher than the control (8.87 mg g(-1) biomass). Co-culture can lead to the generation of complex biomasses with higher added value for developing aquafeed supplements.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/md21030142
Formato Recurso	PDF
Palabras Claves	Aurantiochytrium PUFA LAB co-culture protist
Ubicación del archivo	http://dx.doi.org/10.3390/md21030142
Categoría OCDE	Farmacología y farmacia
Materias	Aurantioquitrio AGPI LABORATORIO cocultura protista
Versión del recurso (Recomendado-único)	versión publicada
Tipo de ruta	dorada verde
Access Rights	acceso abierto
Derechos de acceso	acceso abierto
Id de Web of Science	WOS:000960049000001
Identificador del recurso (Mandatado-único)	artículo original
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Categoría WOS	Farmacología y farmacia
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID-FONDEF IDEA ID22I10307 UCT VIPUCT2019PROSV05 UCT VIPUCT2021PROWB01 MINEDUC UCT21102 ANID FONDEF IDEA ID22I10307 Universidad Católica de Temuco UCT VIPUniversidad Católica de Temuco UCT2019PROSV05 Universidad Católica de Temuco UCT VIPUniversidad Católica de Temuco UCT2021PROWB01 MINEDUC Universidad Católica de Temuco UCT21102