Protist-Lactic Acid Bacteria Co-Culture as a Strategy to Bioaccumulate Polyunsaturated Fatty Acids in the Protist Aurantiochytrium sp. T66
| Primer Autor |
Viscardi, Sharon
|
| Co-autores |
Marileo, Luis
Acuna, Jacqueline
Rilling, Joaquin
Diaz, Paola
Langellotti, Antonio Luca
Russo, Giovanni Luca
Barra, Patricio Javier
Dantagnan, Patricio
|
| Título |
Protist-Lactic Acid Bacteria Co-Culture as a Strategy to Bioaccumulate Polyunsaturated Fatty Acids in the Protist Aurantiochytrium sp. T66
|
| Editorial |
MDPI
|
| Revista |
MARINE DRUGS
|
| Lenguaje |
en
|
| Resumen |
Thraustochytrids are aquatic unicellular protists organisms that represent an important reservoir of a wide range of bioactive compounds, such as essential polyunsaturated fatty acids (PUFAs) such as arachidonic acid (ARA), docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), which are involved in the regulation of the immune system. In this study, we explore the use of co-cultures of Aurantiochytrium sp. and bacteria as a biotechnological tool capable of stimulating PUFA bioaccumulation. In particular, the co-culture of lactic acid bacteria and the protist Aurantiochytrium sp. T66 induce PUFA bioaccumulation, and the lipid profile was evaluated in cultures at different inoculation times, with two different strains of lactic acid bacteria capable of producing the tryptophan dependent auxins, and one strain of Azospirillum sp., as a reference for auxin production. Our results showed that the Lentilactobacillus kefiri K6.10 strain inoculated at 72 h gives the best PUFA content (30.89 mg g(-1) biomass) measured at 144 h of culture, three times higher than the control (8.87 mg g(-1) biomass). Co-culture can lead to the generation of complex biomasses with higher added value for developing aquafeed supplements.
|
| Fecha Publicación |
2023
|
| Tipo de Recurso |
artículo original
|
| doi |
10.3390/md21030142
|
| Formato Recurso |
PDF
|
| Palabras Claves |
Aurantiochytrium
PUFA
LAB
co-culture
protist
|
| Ubicación del archivo | |
| Categoría OCDE |
Farmacología y farmacia
|
| Materias |
Aurantioquitrio
AGPI
LABORATORIO
cocultura
protista
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| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| License |
CC BY 4.0
|
| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
|
| Access Rights |
acceso abierto
|
| Id de Web of Science |
WOS:000960049000001
|
| Tipo de ruta |
verde# dorado
|
| Categoría WOS |
Farmacología y farmacia
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID-FONDEF IDEA ID22I10307
UCT VIPUCT2019PROSV05
UCT VIPUCT2021PROWB01
MINEDUC UCT21102
ANID FONDEF IDEA ID22I10307
Universidad Católica de Temuco UCT VIPUniversidad Católica de Temuco UCT2019PROSV05
Universidad Católica de Temuco UCT VIPUniversidad Católica de Temuco UCT2021PROWB01
MINEDUC Universidad Católica de Temuco UCT21102
|
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