Repositorio UFRO · Omeka S

Melanin Induction Restores the Pathogenicity of Gaeumannomyces graminis var. tritici in Wheat Plants

Primer Autor	Duran, Paola
Co-autores	Aranda, Camila Mendez, Isabel Barra, Patricio Javier Hernandez-Montiel, Luis Fallard, Ana Tortella, Gonzalo Briones, Evelyn
Título	Melanin Induction Restores the Pathogenicity of Gaeumannomyces graminis var. tritici in Wheat Plants
Editorial	MDPI
Revista	JOURNAL OF FUNGI
Lenguaje	en
Resumen	One of the most challenging aspects of long-term research based on microorganisms is the maintenance of isolates under ex situ conditions, particularly the conservation of phytopathological characteristics. Our research group has worked for more than 10 years with Gaumannomyces graminis var. tritici (Ggt), the main biotic factor affecting wheat. In this sense we preserved the microorganisms in oil overlaid. However, several strains preserved for a long time lost their pathogenicity. These strains show white and non-infective mycelia. In this sense, we hypothesized that this is attributable to low melanin content. Melanin is a natural pigment mainly involved in UV protection, desiccation, salinity, oxidation, and fungal pathogenicity. Therefore, understanding the melanin role on Ggt pathogenicity is fundamental to developing melanin activation strategies under laboratory studies. In this study, we induce melanin activation by UV-A light chamber, 320 to 400 nm (T1) and temperature changes of 30 degrees C, 15 degrees C, and 20 degrees C (T2). Fungal pathogenicity was evaluated by determination of blackening roots and Ggt was quantified by real-time PCR in inoculated wheat plants. Results revealed that Ggt grown under UV-A (T1) conditions showed around 40% higher melanin level with a concomitant effect on root infection (98% of blackened roots) and 4-fold more Ggt genome copy number compared with the control (non-infective mycelia) being T1, a more inductor factor compared with T2. These findings would support the role of melanin in pathogenicity in darkly pigmented fungi such as Ggt and could serve as a basis for activating pathogenicity under laboratory conditions.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/jof9030350
Formato Recurso	PDF
Palabras Claves	take-all disease melanin UV vis spectrum
Ubicación del archivo	http://dx.doi.org/10.3390/jof9030350
Categoría OCDE	Microbiología Micología
Materias	enfermedad take.all melanina rayos ultravioleta frente al espectro
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:000956172000001
Tipo de ruta	verde# dorado
Categoría WOS	Microbiología Micología