Repositorio
Institucional

Garcia-Cancino, Apolinaria

Carvajal, Romina I.

Silva-Mieres, Fabiola

Ilabaca, Alejandra

Rocha, Jorge

Arellano-Arriagada, Luciano

Arbalti, Felipe A. Zuniga

Isolation and characterization of Lactobacillus casei A14.2, a strain with immunomodulating activity on Apis mellifera

ELSEVIER

SAUDI JOURNAL OF BIOLOGICAL SCIENCES

en

Considering the economic and environmental role played by bees and their present threats it is necessary to develop food supplements favoring their health. The aim of this work was to isolate and characterize an immunomodulating probiotic capable to improve the health of honeybee colonies. For this purpose, bacterial strains were isolated from Apis mellifera bees (N = 180) obtained at three apiaries. A total of 44 strains were isolated and 9 of them were identified as Lactobacillus having the capacity to grow under saccharose osmotic stress, at pH 4.0 and possessing a wide susceptibility to antibiotics. Results allowed to select two strains but finally only one of them, strain A14.2 showed a very significant immunomodulating activity. This strain increased the expression of mRNA codifying the antimicrobial peptides 24 h postadministration. We evaluated its growth kinetics under aerobic and microaerobic conditions and its survival in the presence of high concentrations of saccharose. Results demonstrated that Lactobacillus casei A14.2 strain was highly tolerant to oxygen and that it was able to adapt to saccharose enriched environments (50% and 100% w/v). Finally, L. casei A14.2 strain was administered monthly during summer and early fall to 4 honeybee colonies (2 controls and 2 treatments). The results showed a gradual sustained decrease of infestation (p < 0.05) by the pathogenic Nosema spp. but no reduction in the infestation by the mite Varroa destructor. These results suggest that the administration of this potential probiotic, may increase the resistance of honeybee colonies to infectious diseases caused by Nosema spp. (c) 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

2023

artículo original

10.1016/j.sjbs.2023.103612

PDF

Apis mellifera

Probiotic

Immunomodulation

Antimicrobial peptides

Lactobacillus casei

Ciencias biológicas y biomedicina: otros temas

Apis mellifera

Probiótico

Inmunomodulación

Péptidos antimicrobianos

Lactobacillus casei

artículo original

versión publicada

CC BY-NC-ND 4.0

CC BY-NC-ND 4.0

acceso abierto

acceso abierto

WOS:000955131600001

1319-562X

Verde# dorado

Ciencias biológicas y biomedicina: otros temas

FIA PYT-2017-0472