Isolation and characterization of Lactobacillus casei A14.2, a strain with immunomodulating activity on Apis mellifera
| Primer Autor |
Garcia-Cancino, Apolinaria
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| Co-autores |
Carvajal, Romina I.
Silva-Mieres, Fabiola
Ilabaca, Alejandra
Rocha, Jorge
Arellano-Arriagada, Luciano
Arbalti, Felipe A. Zuniga
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| Título |
Isolation and characterization of Lactobacillus casei A14.2, a strain with immunomodulating activity on Apis mellifera
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| Editorial |
ELSEVIER
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| Revista |
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
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| Lenguaje |
en
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| Resumen |
Considering the economic and environmental role played by bees and their present threats it is necessary to develop food supplements favoring their health. The aim of this work was to isolate and characterize an immunomodulating probiotic capable to improve the health of honeybee colonies. For this purpose, bacterial strains were isolated from Apis mellifera bees (N = 180) obtained at three apiaries. A total of 44 strains were isolated and 9 of them were identified as Lactobacillus having the capacity to grow under saccharose osmotic stress, at pH 4.0 and possessing a wide susceptibility to antibiotics. Results allowed to select two strains but finally only one of them, strain A14.2 showed a very significant immunomodulating activity. This strain increased the expression of mRNA codifying the antimicrobial peptides 24 h postadministration. We evaluated its growth kinetics under aerobic and microaerobic conditions and its survival in the presence of high concentrations of saccharose. Results demonstrated that Lactobacillus casei A14.2 strain was highly tolerant to oxygen and that it was able to adapt to saccharose enriched environments (50% and 100% w/v). Finally, L. casei A14.2 strain was administered monthly during summer and early fall to 4 honeybee colonies (2 controls and 2 treatments). The results showed a gradual sustained decrease of infestation (p < 0.05) by the pathogenic Nosema spp. but no reduction in the infestation by the mite Varroa destructor. These results suggest that the administration of this potential probiotic, may increase the resistance of honeybee colonies to infectious diseases caused by Nosema spp. (c) 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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| Fecha Publicación |
2023
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| Tipo de Recurso |
artículo original
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| doi |
10.1016/j.sjbs.2023.103612
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| Formato Recurso |
PDF
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| Palabras Claves |
Apis mellifera
Probiotic
Immunomodulation
Antimicrobial peptides
Lactobacillus casei
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| Ubicación del archivo | |
| Categoría OCDE |
Ciencias biológicas y biomedicina: otros temas
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| Materias |
Apis mellifera
Probiótico
Inmunomodulación
Péptidos antimicrobianos
Lactobacillus casei
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| Identificador del recurso (Mandatado-único) |
artículo original
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| Versión del recurso (Recomendado-único) |
versión publicada
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| License |
CC BY-NC-ND 4.0
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| Condición de la licencia (Recomendado-repetible) |
CC BY-NC-ND 4.0
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| Derechos de acceso |
acceso abierto
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| Access Rights |
acceso abierto
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| Id de Web of Science |
WOS:000955131600001
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| ISSN |
1319-562X
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| Tipo de ruta |
Verde# dorado
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| Categoría WOS |
Ciencias biológicas y biomedicina: otros temas
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
FIA PYT-2017-0472
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