Repositorio
Institucional

Uquiche, Edgar

Marillan, Claudia

Extraction of bioactive compounds from Leptocarpha rivularis stems by three-stage sequential supercritical extraction in fixed bed extractor using CO2 and ethanol-modified CO2

ELSEVIER

JOURNAL OF SUPERCRITICAL FLUIDS

en

Sequential supercritical extraction experiments were carried out in three stages each using CO2 and ethanol-modified-CO2, to improve the extraction yield of bioactive compounds from L. rivularis stems. Four experiments were carried out, each in three stages, combining different levels of temperature (40 or 60 degrees C) and pressure (20-50 MPa). The increase in pressure and use of a cosolvent increased the total yield of extract and target compounds compared to one-stage extraction. The highest extraction yields of extract (6.29 g/kg d.s.), terpenes (699.9 mg/kg d.s.), flavonoids (741.9 mg/kg d.s.), and alkaloids (6.22 mg/kg d.s.) were obtained in Experiment 4. Sequential extraction of compounds was superior to one-stage extraction using CO2, but inferior when using ethanol-modified CO2. Both sequential and one-stage cumulative extraction curves were modeled, and the effective diffusivity coefficients ranged between 1.51 and 63.3 x 10-13 m2/s, being highest in Stage 3 where the extraction yield was greatest.

2023

artículo original

10.1016/j.supflu.2023.105903

PDF

Sequential supercritical extraction

Fixed bed extractor

Diffusion model

Bioactive compounds

Ethanol cosolvent

Ingeniería química

Extracción supercrítica secuencial

Extractor de cama fija

Modelo de difusión

Compuestos bioactivos

Cosolvente de etanol

artículo original

versión publicada

metadata

metadata

WOS:000955304200001

0896-8446

Ingeniería química

ANID-FONDECYT 21201441

ANID-FONDECYT 1170841

UFRO DI21-0107

Universidad de La Frontera (UFRO) DI21-0107